Quantitative Determination of Conjugated Bile Acids in Serum in Acute Hepatitis

Frosch, B.; Wagener, H.

doi:10.1038/213404a0

Cited by 23 publications

(8 citation statements)

References 3 publications

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“…Elevation of BA concentrations is a sensitive index of hepatobiliary dysfunction and may be present when other variables, including bromsulfophthalein excretion are within normal limits [8,18].…”

Section: Discussionmentioning

confidence: 99%

Bile Acid Concentrations in Serum during Infancy and Childhood

Sandberg

1970

Pediatr Res

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ExtractConcentrations of free and conjugated bile acids in cord blood (30 samples) were within the adult range and were significantly higher than in serum from newborns (15 infants). Deoxycholic acid (D) was absent from sera of newborns and present in 7 of 23 cord samples. Only two of nine infants in year 1 of life showed detectable amounts of D. In 25 children, ages 1-13 years, bile acid concentrations in serum fell within the normal adult range. Small amounts of unconjugated bile acids were present in some specimens.Thirty-three children with cystic fibrosis of the pancreas (ages 2-14 years) had serum concentrations within the normal adult range. No correlation with age, sex, or severity of disease was noted.Thirty-two children with a variety of hepatobiliary disorders had significantly elevated concentrations of bile acids with the exception of a few infants with terminal cirrhosis. Determination of patterns and concentrations of bile acids in serum are of limited usefulness in differential diagnosis of these disorders. Speculation

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Section: Discussionmentioning

confidence: 99%

Bile Acid Concentrations in Serum during Infancy and Childhood

Sandberg

1970

Pediatr Res

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“…Indeed, it has been suggested that raised serum bile acids are among the most sensitive markers of liver disease (Frosch and Wagener, 1967;Barnes et al, 1975;Isaacs et al, 1976). This conclusion is based mainly on measurements of serum total bile acids but in a few studies the diagnostic value of serum individual bile acids has also been stressed Javitt and Lavy, 1975;Demers and Hepner, 1976;Isaacs et al, 1976).…”

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confidence: 99%

Physiological factors influencing serum bile acid levels

Leòn¹,

Murphy²,

Dowling³

1978

Gut

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SUMMARY This study defines the effects of fasting (prolongation of an overnight fast for a further four hours), feeding (the response to eating the three main 'solid' meals of the day), and cholecystokinin-induced gallbladder contraction (75-100 units of CCK given as a bolus intravenous injection) on serum individual bile acids in five to eight healthy control subjects. The serum conjugates of the two primary bile acids, cholic and chenodeoxycholic, were measured using sensitive specific radioimmunoassays. During fasting, there was no significant change in the levels of the serum individual bile acids (conjugates of cholate, 1 28 + 0 19; conjugates of chenodeoxycholate, 1-17 ± 0-17 ,mol/l).After breakfast, the serum conjugates of cholate and chenodeoxycholate increased significantly but thereafter the mean values remained high with less consistent responses to lunch and dinner, some subjects showing a peak and trough response to all three meals, while others showed a plateau response throughout the day. After breakfast, the serum chenodeoxycholate conjugates increased more rapidly (peak at 60 minutes when the concentration reached 2-07 + 0 30 umol/l) and to a greater extent than the conjugates of cholate (peak at 90 minutes; 1-50 0-24 ,mol/l). A similar pattern of results was seen after intravenous CCK, suggesting either preferential jejunal absorption of chenodeoxycholate conjugates and/or preferential hepatic clearance of cholate conjugates. These results provide essential background data for future studies of serum individual bile acids in intestinal and hepatic disease.

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“…In the method to be described the procedure of Frosch and Wagener (1967) is used: in this, after extraction of serum with hot ethanol, neutral lipids are almost completely removed by distribution in alkaline aqueous alcohol and ether-heptane. By combining the enzymatic conversion of bile acids with the fluorimetric determination of NADH it is possible to measure concentrations of bile acids as low as those found in normal serum.…”

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confidence: 99%

A fluorimetric and enzymatic method for the estimation of serum total bile acids

Murphy¹,

Billing²,

Baron³

1970

J Clin Pathol

233

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The enzymatic technique using 3α-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4·7 μmol/litre for males and 1·0-8·2 μmol/litre for females.

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Quantitative Determination of Conjugated Bile Acids in Serum in Acute Hepatitis

Cited by 23 publications

References 3 publications

Bile Acid Concentrations in Serum during Infancy and Childhood

Bile Acid Concentrations in Serum during Infancy and Childhood

Physiological factors influencing serum bile acid levels

A fluorimetric and enzymatic method for the estimation of serum total bile acids

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