Quantitative determination of capsaicin in liniments by microemulsion liquid chromatography

Pashkova, E. B.; Пирогов, А. В.; Yunovidov, D. V.; Шпигун, О. А.

doi:10.3103/s002713141101010x

Cited by 9 publications

(2 citation statements)

References 3 publications

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“…The surfactants in the microemulsion solubilize/bind proteins, which reduces sample pretreatment needs for analysis of biological fluids such as plasma. Finally, the potential of microemulsion applications as mobile phases in LC analysis was proven .…”

Section: Introductionmentioning

confidence: 99%

“…sample pretreatment needs for analysis of biological fluids such as plasma. Finally, the potential of microemulsion applications as mobile phases in LC analysis was proven [24,25]. The problem of microemulsion liquid chromatography (MELC) is that many variables need to be optimized such as surfactant type/concentration, oil type/concentration and so on.…”

Section: Introductionmentioning

confidence: 99%

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Multiobjective optimization strategy based on desirability functions used for the microemulsion liquid chromatographic separation and quantification of norfloxacin and tinidazole in plasma and formulations

Abou-Taleb

El‐Wasseef

El-Sherbiny

et al. 2015

J of Separation Science

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The aim of the present study was to optimize a microemulsion liquid chromatography method for the simultaneous determination of norfloxacin and tinidazole binary mixture using a chemometric protocol. Optimization experiments were conducted through a process of screening and optimization. A 2(7-4) fractional factorial design was used as screening design. While the location of optimum conditions was established by applying Derringer's desirability function. The optimal mobile phase composition was predicted to be: 3.5% w/v SDS, 10.03% v/v 1-propanol, 0.5% v/v 1-octanol, and 0.3% triethylamine in 0.02 M phosphoric acid at pH 6.5. The mobile phase was delivered isocratically at a flow rate of 1 mL/min with UV detection at 290 nm. Tinidazole and norfloxacin were eluted with retention times of 1.8 and 5.8 min, respectively. The calibration plots displayed good linear relationships in the concentration ranges of 0.5-50 and 0.75-75 μg/mL for norfloxacin and tinidazole, respectively. The method was successfully applied for determination of both drugs in pharmaceutical dosage forms and real human plasma. Where the accuracy was proved by the low values of % error and high values of recovery, also the relative standard deviation for the results did not exceed 1.5%, proving the precision of the method.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%