Quantitative determination of amikacin Sulfate using vanillin from pure and commercial brands available in Pakistan

An aminoglycoside antibiotic, amikacin, is used to treat severe and recurring bacterial infections. Due to the absence of a chromophore, however, amikacin must be extensively derivatized before being quantified, both in analytical and bioanalytical samples. In this study, for the first time, we developed a simple and sensitive method for measuring amikacin sulfate using spectrofluorimetry with a 96-well plate reader, based on the design of the experiment's approach. To develop a robust and reproducible spectrofluorimetric method, the influence of essential attributes, namely pH of the buffer, heating temperature, and concentration of reagents, were evaluated using univariate analysis followed by multivariate analysis (central composite design).International Conference of Harmonization guidelines were used to validate the optimized method. The developed technique is linear from 1.9 to 10 μg/ml with a regression coefficient of 0.9991. The detection and quantification limits were 0.649 μg/ml and 1.9 μg/ml, respectively. For the developed method, both intraday and interday precision (%RSD) were less than 5%. Using the method, amikacin concentrations were quantified in prepared amikacin liposomes and commercial formulations of Amicin ® . The developed method greatly reduces sample volume and is a rapid, high throughput microplate-based fluorescence approach for the convenient and costeffective measurement of amikacin in pharmaceutical formulations. In comparison with previously published approaches, the suggested method allowed for quick analysis of a high number of samples in a short amount of time (96 samples in 125 sec), resulting in an average duration of analysis of 1.3 sec per sample.

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A novel high throughput 96‐well‐based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology

Maxwell

Mary

Ghate

et al. 2022

Luminescence

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Green Simple Spectrophotometric Methods for Determination of Kanamycin Sulfate using Eosin and Vanillin Reagents

Elnaggar

Gawad

Belal

2019

Analytical Chemistry Letters

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Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms

Kalyani¹,

Rao²

2018

Braz. J. Pharm. Sci.

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A simple, accurate, isocratic stability indicating RP-HPLC method was developed for the determination of cefepime and amikacin in Pure and its pharmaceutical formulations. The method consists of methanol: acetonitrile:acetate buffer 75:20:05 (v/v) mobile phase at pH 5.1 with C18 column as stationary phase. The flow rate and detection wave length were 1.0 mL/min and 212 nm respectively. The linearity range for the method was found to be 2.5-25 µg/mL for amikacin and 10-100 µg/mL cefepime respectively. The developed method was validated as per ICH guidelines and the results of all the validation parameters were well within their acceptance values. Also the forced degradation studies were conducted with standard drugs. Degradation products formed during the different stress conditions were separated from both drugs. This validated method was applied for the simultaneous estimation of cefepime and amikacin in commercially available formulation sample.

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Quantitative determination of amikacin Sulfate using vanillin from pure and commercial brands available in Pakistan

Cited by 5 publications

References 12 publications

A novel high throughput 96‐well‐based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology

A novel high throughput 96‐well‐based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology

Green Simple Spectrophotometric Methods for Determination of Kanamycin Sulfate using Eosin and Vanillin Reagents

Stability indicating RP-HPLC method development and validation of cefepime and amikacin in pure and pharmaceutical dosage forms

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