Quantitative detection and diversity of the pyrrolnitrin biosynthetic locus in soil under different treatments

Garbeva, Paolina; Voesenek, Krysta; Elsas, Jan Dirk van

doi:10.1016/j.soilbio.2004.03.009

Cited by 45 publications

(43 citation statements)

References 31 publications

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“…However, on the basis of in silico analysis of fully sequenced B. cepacia complex genomes included in the 16S rRNA gene and recA phylogeny in this study ( Fig. 1 and 2 (49,66) and has been postulated to play a role in the natural suppressiveness of some soils against root rot caused by Rhizoctonia solani (32). To further clarify the relative importance of pyrrolnitrin production by the endophytic Burkholderia isolates in growth inhibition of F. moniliforme, site-directed mutagenesis of the prnD gene was performed with Burkholderia isolate ESS4.…”

Section: Resultsmentioning

confidence: 89%

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Mendes

Pizzirani-Kleiner

Araújo

et al. 2007

Appl Environ Microbiol

211

114

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Bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in Brazil. Endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. Many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16S rRNA gene sequence analysis revealed that the selected isolates of the endophytic bacterial community of sugarcane belong to the genera of Burkholderia, Pantoea, Pseudomonas, and Microbacterium. Bacterial isolates belonging to the genus Burkholderia were the most predominant among the endophytic bacteria. Many of the Burkholderia isolates produced the antifungal metabolite pyrrolnitrin, and all were able to grow at 37°C. Phylogenetic analyses of the 16S rRNA gene and recA gene sequences indicated that the endophytic Burkholderia isolates from sugarcane are closely related to clinical isolates of the Burkholderia cepacia complex and clustered with B. cenocepacia (gv. III) isolates from cystic fibrosis patients. These results suggest that isolates of the B. cepacia complex are an integral part of the endophytic bacterial community of sugarcane in Brazil and reinforce the hypothesis that plant-associated environments may act as a niche for putative opportunistic human pathogenic bacteria.Brazil is one of the world's largest sugarcane producers and has considerable influence over the international sugar market. Brazilian sugarcane is primarily used to produce sugar and alcohol. Production has increased over time to approximately 26 million tons of sugar and 16 million m 3 of alcohol in 2006. Although ethanol has been used as an alternative source of fuel in Brazil since 1980, it is currently receiving worldwide interest as a biofuel to replace, at least in part, gasoline, thereby contributing to a reduction in carbon emissions (33). Consequently, sustaining and enhancing the growth and yield of sugarcane have become a major focus of research. The growth and performance of sugarcane in the field are adversely affected by a number of abiotic and biotic factors, including a wide range of fungal and bacterial diseases. Pokkah boeng, caused by the fungus Fusarium moniliforme, is one of the most widespread diseases and may cause serious yield losses in commercial sugarcane plantings (68). F. moniliforme can be disseminated horizontally by airborne spores or crop debris and vertically through seed pieces. Current control strategies involve the use of resistant varieties and fungicide applications. The efficacy of both control measures, however, is limited, and there is an increasing need for novel and environmentally sound strategies to control this and other diseases of sugarcane.The overall goal of this study was to isolate and characterize beneficial bacteria that are intimately associated with sugarcane and have the potential to control pathogens and to promote the growth and yie...

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Section: Resultsmentioning

confidence: 89%

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Mendes

Pizzirani-Kleiner

Araújo

et al. 2007

Appl Environ Microbiol

211

114

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“…Many workers have used genomic molecular markers to track the biocontrol strain (Raaijmakers and Weller 2001;Garbeva et al 2004;Broggini et al 2005). This technique has drawbacks, as the native strain may also have similar molecular markers.…”

Section: Molecular Monitoring Methodsmentioning

confidence: 99%

Rhizosphere and Root Colonization by Bacterial Inoculants and Their Monitoring Methods: A Critical Area in PGPR Research

Ahmad

Husain²,

Ahmad³

2011

Microbes and Microbial Technology

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Roots serve a multitude of functions in plants including anchorage, acquisition of nutrients and water, and production of exudates with growth regulatory properties. The root-soil interface, or rhizosphere, is the site of greatest biological and chemical activity within the soil matrix. Plant growth-promoting rhizobacteria (PGPR) are known to influence plant health by controlling plant pathogens or via direct enhancement of plant development in the laboratory and in greenhouse experiments. Unfortunately, however, results in the field have been less consistent. The colonization of roots by inoculated bacteria is an important step in the interaction between beneficial bacteria and the host plant. However, colonization is a complex phenomenon influenced by many biotic and abiotic parameters, some of which are only now apparent. Monitoring fate and metabolic activity of microbial inoculants as well as their impact on rhizosphere and soil microbial communities are needed to guarantee safe and reliable application, independent of whether they are genetically modified or not. The first and most crucial prerequisite for effective use of PGPRs is that strain identity and activity are continuously confirmed. A combination of both classical and molecular techniques must be perfected for more effective monitoring of inoculants strain (both genetically modified and unmodified) after release into the soil. Recent developments in techniques for studying rhizobacterial communities and detection and tracking systems of inoculated bacteria are important in future application and assessment of effectiveness and consistent performance of microbial inoculants in crop production and protection.

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“…Other aspects of plant litter chemistry, such as glucosinolate content, can also have a dramatic and long-term effect on the soil microbial community (Mazzola et al 2015). Stimulating soil saprotrophic communities with a diverse composition of plant litter may reduce soil-borne pathogens, either through increased competition for resources (Fontaine et al 2003) or by increasing the abundance of beneficial, pathogen antagonistic microbes (Garbeva et al 2004b). …”

Section: Plant Litter Affects Decomposer Communitymentioning

confidence: 99%

Cover crops to increase soil microbial diversity and mitigate decline in perennial agriculture. A review

Vukicevich

Lowery

Bowen

et al. 2016

Agron. Sustain. Dev.

267

157

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Quantitative detection and diversity of the pyrrolnitrin biosynthetic locus in soil under different treatments

Cited by 45 publications

References 31 publications

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Diversity of Cultivated Endophytic Bacteria from Sugarcane: Genetic and Biochemical Characterization of Burkholderia cepacia Complex Isolates

Rhizosphere and Root Colonization by Bacterial Inoculants and Their Monitoring Methods: A Critical Area in PGPR Research

Cover crops to increase soil microbial diversity and mitigate decline in perennial agriculture. A review

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