Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients

Bretagne, Stéphane; Costa, Jean Marc; Fleury-Feith, J; Poron, Françoise; Dubreuil‐Lemaire, Marie‐Laure; Vidaud, Michel

doi:10.1128/jcm.33.6.1662-1664.1995

Cited by 46 publications

(11 citation statements)

References 9 publications

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“…22 In humans, diagnosis is further facilitated by PCR and tissue culture of BAL fluid. 23 Serology may be used as an adjunct to diagnosing toxoplasmosis though interpretation is complicated. A positive IgM titre or a 4fold or greater increase in IgG in paired serum samples taken 2 to 4 weeks apart is generally considered diagnostic of active toxoplasmosis.…”

Section: Discussionmentioning

confidence: 99%

Antemortem diagnosis and treatment of toxoplasmosis in two cats on cyclosporin therapy

2006

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Clinical toxoplasmosis was diagnosed antemortem in two cats being treated with therapeutic doses of cyclosporin. The diagnosis was made by detecting tachyzoites on cytological examination of bronchoalveolar lavage fluid from one case and pleural effusion from the other. Despite early diagnosis and aggressive treatment in both cases, only one cat survived. Reactivation of latent Toxoplasma gondii infection secondary to cyclosporin-induced immunosuppression was considered likely in both cases. The presence of respiratory signs in cats treated with cyclosporin should alert clinicians to the possibility of clinical toxoplasmosis. Consideration should be given to determining the serostatus of cats to T gondii prior to use of drugs which are potent inhibitors of cell mediated immunity, such as cyclosporin. Two cases of feline toxoplasmosis are presented.

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Section: Discussionmentioning

confidence: 99%

Antemortem diagnosis and treatment of toxoplasmosis in two cats on cyclosporin therapy

2006

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“…PCR has been used to detect T. gondii in various biological samples of HIV-infected patients (1,3,5,6,9,13,14,15,16,18,20). Most of these studies were done by examining cerebrospinal fluid.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies used cerebrospinal fluid; the sensitivities were between 40 and 50% for this target (6,8,(21)(22)(23)25). In other studies brain or liver tissue, bronchoalveolar lavage, lymph nodes, and blood samples were used but, especially for the latter, only from limited number of patients (1,6,7,12,14,17,20,24,26,28). Most of these samples can be obtained only by invasive procedures, and only blood can be easily obtained from the patients.…”

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confidence: 99%

Limited value of PCR for detection of Toxoplasma gondii in blood from human immunodeficiency virus-infected patients

et al. 1997

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Cerebral toxoplasmosis is a common, opportunistic, and often life-threatening disease in HIV-infected patients. Diagnosis is supported mainly by clinical evidence and computerized tomography or magnetic resonance imaging scans, but brain images may share features with other brain diseases occurring in HIVinfected patients. To determine the diagnostic value of PCR for the detection of Toxoplasma gondii in blood from HIV-infected patients, we examined 89 blood samples from 59 HIV-infected patients. PCR and Southern blot hybridization were done with DNA extracted from blood samples from 20 patients with confirmed cerebral toxoplasmosis and from 10 patients with suspected but not confirmed cerebral toxoplasmosis. The samples were taken before and 7 to 10 days after the beginning of antiparasitic therapy. For 9 patients who suffered from cerebral toxoplasmosis more than 6 months prior to the study and for 20 patients without any evidence for toxoplasmosis only one blood sample per patient was examined. PCR gave positive results with 5 of the 20 blood samples from patients who suffered from cerebral toxoplasmosis. After 7 to 10 days of therapy PCR results became negative in all these five cases. No amplification was seen with DNA from blood samples from the other 54 patients as the target. The results presented here show that PCR testing of blood samples from HIV-infected patients is of limited value for the diagnosis of cerebral toxoplasmosis. The sensitivity was only 25%, but the specificity was very high (100%), so this technique may be useful for discriminating between cerebral toxoplasmosis and other brain diseases which may be mistaken for toxoplasmosis.

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“…Além disso, esta reação tem também aplicabilidade na detecção de T.gondii em nível ocular, no sangue de imunodeficientes (Filice et al 37 ; Dupouy-Camet et al 38 . ; Dupon et al 39 ), no lavado bronco-pulmonar (Bretagne et al 40 ), no líquido amniótico (Grover et al 41 ) e na urina. (Fachado et al 42 ).…”

Section: Unitermosunclassified

Toxoplasmose: ocorrência de anticorpos antitoxoplasma gondii e diagnóstico

Cantos¹,

Prando²,

Siqueira³

et al. 2000

Rev. Assoc. Med. Bras.

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The results showed that 41.91% of the patients had the antibodies anti-Toxoplasma gondii. From this total we concluded that 0.87% of these patients had the IgM in different titles which can be or not classified in the acute phase of the disease. This study demonstrates that the incidence of toxoplasmosis in our orment is relatively high. It shows too that in the identification of the acute process of the disease, the serologic tests can be added to a more sensible and specific techniques that allows the evidentiation of the microorganism thus contributing to a better diagnosis.

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Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients

Cited by 46 publications

References 9 publications

Antemortem diagnosis and treatment of toxoplasmosis in two cats on cyclosporin therapy

Antemortem diagnosis and treatment of toxoplasmosis in two cats on cyclosporin therapy

Limited value of PCR for detection of Toxoplasma gondii in blood from human immunodeficiency virus-infected patients

Toxoplasmose: ocorrência de anticorpos antitoxoplasma gondii e diagnóstico

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