Quantitative Coherent Anti-Stokes Raman Scattering (CARS) Microscopy

Day, James P. R.; Domke, Katrin F.; Rago, Gianluca; Kano, Hideaki; Hamaguchi, Hiro O.; Vartiainen, Erik M.; Bonn, Mischa

doi:10.1021/jp200606e

Cited by 126 publications

(100 citation statements)

References 65 publications

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“…Additionally, the CARS signal generally presents slightly dissipative spectral lineshape, which is ascribed to the weak nonresonant backgrounds and well-understood in the CARS community. Because the dual-comb CARS is a spectroscopic technique, vibrational phase, which excludes the nonresonant contribution, can be extracted mathematically from the CARS signal intensity [5,[37][38]. Fig.…”

Section: Resultsmentioning

confidence: 99%

Spectral focusing dual-comb coherent anti-Stokes Raman spectroscopic imaging

Chen

et al. 2017

Opt. Lett.

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Coherent Raman microscopy provide label-free imaging by interrogating the intrinsic vibration of biomolecules.Nevertheless, trade-off between high chemical-specificity and high imaging-speed currently exists in transition from spectroscopy to spectroscopic imaging when capturing dynamics in complex living systems. Here, we present a novel concept in dual-comb scheme to substantially beat this trade-off and facilitate high-resolution broadband coherent antiStokes Raman spectroscopic imaging based on down-converted, automatically varying delay-time in spectral focusing excitation. A rapid measurement of vibrational microspectroscopy on sub-microsecond scale over a spectral span ~700 cm -1 with solid signal-to-noise ratio provides access to well-resolved molecular signatures within the fingerprint region. We demonstrate this high-performance spectroscopic imaging of spatially inhomogeneous distributions of chemical substances as well as the carotenoids accumulation in plant cells. This technique offers an unprecedented route for broadband CARS imaging with hundreds of kHz frame rate using available 1-GHz oscillators.

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Section: Resultsmentioning

confidence: 99%

Spectral focusing dual-comb coherent anti-Stokes Raman spectroscopic imaging

Chen

et al. 2017

Opt. Lett.

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“…Principal component analysis (PCA) known from linear Raman spectroscopy has been applied to spectral CARS data to visualize differences in the lipid distribution in meibomian glands [46], and to identify cholesterol crystals in both SRS and CARS spectral data [37,47]. Even more sophisticated methods are required to extract the Raman spectrum from multiplex CARS data, e.g., the maximum entropy method or the Kramers Kronig relations [48][49][50].…”

Section: Methodsmentioning

confidence: 99%

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Meyer

Schmitt

Dietzek

et al. 2013

Journal of Biophotonics

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Journal of BIOPHOTONICSMultimodal nonlinear microscopy has matured during the past decades to one of the key imaging modalities in life science and biomedicine due to its unique capabilities of label-free visualization of tissue structure and chemical composition, high depth penetration, intrinsic 3D sectioning, diffraction limited resolution and low phototoxicity. This review briefly summarizes first recent advances in the field regarding the methodology, e.g., contrast mechanisms and signal characteristics used for contrast generation as well as novel image processing approaches. The second part deals with technologic developments emphasizing improvements in penetration depth, imaging speed, spatial resolution and nonlinear labeling strategies. The third part focuses on recent applications in life science fundamental research and biomedical diagnostics as well as future clinical applications.Multimodal nonlinear microscopy of tissue.

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“…The forward CARS signal from samples between two coverslips was collected by another objective (LUMPlanFl/IR 40xW, N.A. = 0.8, Olympus) and delivered into a spectrometer equipped with an EMCCD camera (ProEM 1600 (2) , Princeton Instruments). The exposure time was 1 ms, and 50 acquisitions were averaged.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…Compared to spontaneous Raman scattering, coherent anti-Stokes Raman scattering (CARS) provides coherent signal enhancement, realizing real-time vibrational imaging [1,2]. In CARS, pump and Stokes fields generate molecular vibration, which is scattered off by a probe field to create a blue-shifted anti-Stokes field.…”

Section: Introductionmentioning

confidence: 99%

Broadband nonlinear vibrational spectroscopy by shaping a coherent fiber supercontinuum

Liu¹,

King²,

Tu³

et al. 2013

Opt. Express

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Vibrational spectroscopy has been widely applied in different fields due to its label-free chemical-sensing capability. Coherent anti-Stokes Raman scattering (CARS) provides stronger signal and faster acquisition than spontaneous Raman scattering, making it especially suitable for molecular imaging. Coherently-controlled single-beam CARS simplifies the conventional multi-beam setup, but the vibrational bandwidth and nontrivial spectrum retrieval have been limiting factors. In this work, a coherent supercontinuum generated in an all-normal-dispersion nonlinear fiber is phase-shaped within a narrow bandwidth for broadband vibrational spectroscopy. The Raman spectra can be directly retrieved from the CARS measurements, covering the fingerprint regime up to 1750 cm −1 . The retrieved spectra of several chemical species agree with their spontaneous Raman data. The compact fiber supercontinuum source offers broad vibrational bandwidth with high stability and sufficient power, showing the potential for spectroscopic imaging in a wide range of applications. 1929-1960 (2000). 11. Y. Silberberg, "Quantum coherent control for nonlinear spectroscopy and microscopy," Annu. Rev. Phys. Chem. 60(1), 277-292 (2009 Midorikawa, "Single-pulse coherent anti-Stokes Raman scattering microscopy employing an octave spanning pulse," Opt. Express 17 ( "Cross-validation of theoretically quantified fiber continuum generation and absolute pulse measurement by MIIPS for a broadband coherently controlled optical source," Appl.

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Quantitative Coherent Anti-Stokes Raman Scattering (CARS) Microscopy

Cited by 126 publications

References 65 publications

Spectral focusing dual-comb coherent anti-Stokes Raman spectroscopic imaging

Spectral focusing dual-comb coherent anti-Stokes Raman spectroscopic imaging

Accumulating advantages, reducing limitations: Multimodal nonlinear imaging in biomedical sciences – The synergy of multiple contrast mechanisms

Broadband nonlinear vibrational spectroscopy by shaping a coherent fiber supercontinuum

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