Quantitative Bioimaging of Platinum via Online Isotope Dilution-Laser Ablation-Inductively Coupled Plasma Mass Spectrometry

Abstract

A new calibration strategy for elemental bioimaging based on online isotope dilution analysis (IDA) and laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) was developed and applied for the quantification of platinum in rat kidney tissues. A dry Pt spike aerosol was added in a post-cell setup, and the naturalPt/Pt isotope ratio of the sample aerosol from laser ablation was changed accordingly. Spike mass flow determination was carried out based on reversed IDA using a reference standard. Quantitative data obtained by the new approach correlated well with those obtained by external calibration when analyzing parallel tissue slices of rat kidney from cisplatin perfusion studies. The novel quantification approach is traceable to SI units, as IDA is an definitive method. Signal drifts are compensated as the second isotope acts as an internal standard.

Laser Ablation–Inductively Coupled Plasma–Mass Spectrometry Imaging in Biology

“…Fernandez et al determined the mass fraction by parallel analysis of a CRM with a known concentration 161 and applied the method to the quantification of powdered samples. Using a customized reference material, Bauer et al 162 followed a similar strategy for the spatially resolved quantification of Pt in biological tissue. Pickhardt et al 163 mounted a microflow nebulizer in the ablation cell which mixed the dry sample aerosol with a wet isotopically enriched standard.…”

Combining standard addition and isotope dilution in order to improve SI traceable LA-ICP-MS measurements

“…[2][3][4] Different research and industrial 5 fields are interested in this technique and the areas of application are wide spread. For example, the LA-ICP-MS is used in geology 6 , medicine 7,8 , but also for the provenance analysis 9 or forensic science. 10 Although numerous applications of LA-ICP-MS have been carried out during the last years, it is still challenging to make them quantitative and traceable to the SI (Système international d'unités).…”

Quantitative elemental bioimaging: an antibody-based double-labelling method to quantify the cell-specific distribution of silver nanoparticles in lung tissue sections

“…LA-ICP-MS can provide quantitative results as shown by previous studies in which either matrix-matched external calibration, internal calibration with suitable solutions, or isotope dilution analysis was used. [18][19][20][21] Further prove of this concept came from complementary ICP-MS analyses of standard concentrations aer microwave digestion. 22,23 Since LA-ICP-MS may also be used to detect metal-labelled biological targets [24][25][26] we combined the quantitative mass spectrometric detection of silver with a gold and uorescence-based immunohistochemical detection technique for biological target structures.…”