Quantitative Bestimmung von ?-Aminos�uren in Papierchromatogrammen und Ionopherogrammen mit radioaktivem Kupfer

Ionography: A new frontier in electrophoresis

“…Wieland and Fischer used filter paper strips saturated with an acetate buffer as a medium for electrophoresis, while studying the separation of wimple amino acids and of the complexes formed when various amino acids react with cupric ion (14)-By incorporating radioactive copper into the complexes, cutting the strips into narrow bands and determining their radioactivity, they explored the possibility of using this method as a quantitative technique (15). To maintain the strips at constant temperature and lessen the effects of heating, Cremer and Tiselius used filter paper strips between glass plates and immersed in a bath of chlorobenzene (16).…”

Analytical Nuclear Chemistry

“…Amino acids.-The property of amino acids to form complex compounds with copper was used by Wieland et al (188) for a quantitative determination of a-amino acids. By using CU64 (12.8-hr.)…”

Principles of Isotope Dilution Assays

“…Typical of the application of derivative dilution is the determination of simple amino acids in protein hydrolyzates by the use of p-I131phenylsulfonj'l chloride (32). Other reagents for amino acids are Cu364(P04)2 (6) and Cu64(OAc)2 (65).…”