Ionography: A new frontier in electrophoresis

McDonald, Hugh J.

doi:10.1021/ed029p428

Cited by 48 publications

(8 citation statements)

References 13 publications

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“…Paper electrophoresis was carried out by a modification and combination of several methods (15)(16)(17). A vapor tight plastic chamber was employed and the experiments were conducted at 20 to 250 C. using veronal-citrate buffer pH 8.6, r/2 0.05; a rectangular sheet of Whatman 3 1662…”

Section: Methodsmentioning

confidence: 99%

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Protein-Lipid Relationships in Human Plasma. III. In Pregnancy and the Newborn1

Russ¹,

Eder²,

Barr³

1954

J. Clin. Invest.

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This study of pregnant women and their newborn infants was undertaken as a part of a more extensive investigation of factors that modify the interrelations of proteins and lipids in human plasma (1-3).The composition of maternal and fetal blood has been examined by many observers who, however, have usually focussed attention either on proteins or lipids. Electrophoretic studies of proteins have been made by Longsworth, Curtis, and Pembroke (4) who analyzed both serum and plasma; by Lagercrantz (5) and Moore, DuPan, and Buxton (6) who examined only serum; and by Macy and Mack (7) whose monograph was devoted to an inquiry concerning plasma proteins in human reproduction. There is consensus that maternal plasma has less than normal concentration of albumin and a greater than normal concentration of alpha and beta globulins while gamma globulins are unchanged or only slightly decreased. These electrophoretic analyses of the cord plasma have shown lower than normal concentration of total protein which is chiefly attributable to a marked reduction in the concentration of alpha and beta globulins. Numerous investigators (8-10) have shown that in maternal blood the concentration of cholesterol and phospholipids is greater than normal while in blood from the umbilical cord at the time of birth it is notably reduced (11-13).In the present study, utilization of the microfractionation method No. 10 of Cohn and his coworkers (14) METHODSThe microfractionation method of Cohn and his coworkers (14) was used for separation of proteins. In this study the procedure was carried out in only three stages with isolation of Fraction IV + V + VI, Fraction II, and Fraction I + III. Analyses for protein, cholesterol, and phospholipids were made on the unfractionated plasma and each of its fractions. Because of the extremely small amounts of phospholipids in Fraction II, the determination was omitted.Details of the procedure involving fractionation and the analytical methods for proteins, cholesterol, and phospholipids have been presented in a previous publication (1). They have not been significantly modified for the present work.The distribution of protein components in the various fractions was studied electrophoretically by the moving boundary method and also by paper ionophoresis which was adapted to the purpose of identifying lipoproteins. The moving boundary method was performed on ACD plasma and the three fractions using an Aminco Stern apparatus with a standard analytical cell. Details of this procedure and the preparation of samples prior to electrophoresis have been described in a previous publication. (1).Paper electrophoresis was carried out by a modification and combination of several methods (15)(16)(17). A vapor tight plastic chamber was employed and the experiments were conducted at 20 to 250 C. using veronal-citrate buffer pH 8.6, r/2 0.05; a rectangular sheet of Whatman 3 1662

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Section: Methodsmentioning

confidence: 99%

“…Paper electrophoresis was carried out by a modification and combination of several methods (15)(16)(17) Goa (18); the duplicate section was stained with Sudan black B for lipoproteins according to Swahn (19).…”

Section: Methodsmentioning

confidence: 99%

Protein-Lipid Relationships in Human Plasma. III. In Pregnancy and the Newborn1

Russ¹,

Eder²,

Barr³

1954

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Evaporation is also a function of the type of vessel surrounding the paper strip. Sandwiching the paper between glass plates has been most common, but many investigators now7 suspend it on glass rods (101,271,315,362,501) or hang it free in the air in an enclosed vessel (284).…”

Section: Zone Electrophoresismentioning

confidence: 99%

“…Convection and backelectrophoresis of the products are avoided largely by proper design of the end vessels. Designs range from simple liquid containers with no precautions against these effects to the other limit of elaborate multicompartment cells (315). The matter is clearly of secondary importance except in continuous operation for long periods, because it is largely subject to control by frequent renewal of the solutions in the end vessels.…”

Section: Zone Electrophoresismentioning

confidence: 99%