Protein-Lipid Relationships in Human Plasma. III. In Pregnancy and the Newborn1

Russ, Ella M.; Eder, Howard A.; Barr, David P.

doi:10.1172/jci103047

Cited by 49 publications

(10 citation statements)

References 15 publications

(17 reference statements)

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“…In confirmation of earlier reports (13,33), SPI's in maternal and cord blood, although not identical, were not, in the present study, significantly different. A free passage of all proteins between maternal and fetal circulations is unlikely, since with the exception of gamma globulin, the electrophoretic components of serum obtained from cord blood resemble those of the non-pregnant adult more nearly than those of the mother (34). Nevertheless, it has been demonstrated that various gamma globulins pass from the maternal to the fetal circulation (35).…”

Section: Discussionmentioning

confidence: 99%

Thyroxine-Binding by Sera of Pregnant Women, New-Born Infants, and Women With Spontaneous Abortion 1

Dowling¹,

Freinkel²,

Ingbar³

1956

J. Clin. Invest.

147

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Section: Discussionmentioning

confidence: 99%

Thyroxine-Binding by Sera of Pregnant Women, New-Born Infants, and Women With Spontaneous Abortion 1

Dowling¹,

Freinkel²,

Ingbar³

1956

J. Clin. Invest.

147

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“…Paper electrophoresis was carried out by the method described in detail in a previous publication (14).…”

Section: Methodsmentioning

confidence: 99%

“…Pierce and Gofman (6) examined the sera of thirty-two patients with cirrhosis and found levels of the Sf 10-20 class slightly higher than normal. They also studied forty-eight cases of acute hepatitis (7) and found elevations of the Sf 0-100 components with the largest increase in the Sf [12][13][14][15][16][17][18][19][20] class. The increased concentrations correlated most closely with the presence of jaundice and tended to decrease when the jaundice subsided.…”

mentioning

confidence: 99%

Protein-Lipid Relationships in Human Plasma: In Biliary Cirrhosis, Obstructive Jaundice, and Acute Hepatitis 1

Eder¹,

Russ²,

Pritchett³

et al. 1955

J. Clin. Invest.

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In 1862, Austin Flint (1) found that cholesterol accumulated in the blood in obstructive jaundice.Fifty years later Widal, Weill, and Laudat (2) pointed out that the hypercholesterolemia of obstructive jaundice is due in large measure to increase in serum free cholesterol in contradistinction to that of nephrosis, where both free and esterified cholesterol are greater than normal.More recently (3-5) it has been shown that plasma phospholipid increases even more than cholesterol, so that the ratio of total cholesterol to phospholipid is appreciably less than normal.The serum lipoproteins have also been measured in patients with liver disease. Pierce and Gofman (6) examined the sera of thirty-two patients with cirrhosis and found levels of the Sf 10-20 class slightly higher than normal. They also studied forty-eight cases of acute hepatitis (7) and found elevations of the Sf 0-100 components with the largest increase in the Sf [12][13][14][15][16][17][18][19][20] class. The increased concentrations correlated most closely with the presence of jaundice and tended to decrease when the jaundice subsided. McGinley, Jones, and Gofman (8) found a huge increase in the Sf 6 and Sf 8 groups, with varying increase in Sf 10-17 in five cases of biliary cirrhosis. By starch zone electrophoresis, Kunkel and Slater (9) observed that in biliary cirrhosis there was a high peak consisting predominantly of phospholipid which had the same relative mobility as normal beta lipoproteins. The concentration of alpha lipoproteins was extremely low.

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“…Unfractionated plasma and all Cohn fractions were analyzed for concentrations of cholesterol, phospholipids, and protein and for distribution of electrophoretic components (moving boundary and filter paper methods) as previously described (8,11,17).…”

Section: Analytical Proceduresmentioning

confidence: 99%

Lipoproteins in Primary Biliary Cirrhosis 1

Russ¹,

Raymunt²,

Barr³

1956

J. Clin. Invest.

Self Cite

122

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Evidence gradually accumulated by moving boundary electrophoresis (1-4), chemical fractionation, (5-7) using method of Cohn and his associates (8) biliary cirrhotic ACD 2 plasma and 9 ml. of normal ACD2 plasma were dialyzed against several changes of citrate-sodium chloride reagent8 for 48 hours at 0°C. Densities of the dialyzed solutions were adjusted to approximately 1.065 with 4.0 M NaCl and distilled water until a final volume of 11.0 ml. was obtained for the biliary cirrhotic and 13.0 ml. for the normal plasma. Nineml. aliquots of each were placed in 13 X 85 mm. plastic centrifuge tubes and ultracentrifuged at 30,000 rpm (80,000 G) for 18 to 20 hours at 50 C. The top 3.0 cm. of solution were separated with a tube-slicing device. From the upper and lower cuts containing low and high density lipoproteins, respectively, 100 X samples were removed for determination of solution density. The upper cut of the biliary cirrhotic plasma was diluted to 6.0 ml. with 2.3 M NaCl and from the normal plasma with 2.0 M NaCl. An aliquot of 4.5 ml. was removed from each into 13 X 85 mm. plastic tubes for density gradient studies (described presently); the remainder was dialyzed together with both lower cuts (high density lipoproteins) against 0.15 M NaCl for 24 hours at 0°C. The dialyzed solutions were then analyzed for lipids, nitrogen, and dry weight.A linear density gradient as described by LinderstromLang and Lanz (13,14) and Anfinsen (15) was made of the diluted upper cuts as follows: 4.5 ml. 0.1 M NaCl were layered over the 4.5-ml. aliquot of low density lipoproteins that had been previously placed in a 13 X 85 mm. plastic tube. A simply-constructed wire stirrer with a spiral button tip was gently inserted to the interface of the two solutions, from which point gentle vertical strokes of increasing 0.75 cm. distances were made, keeping the plane of the spiral tip as level as possible. The manipulation usually required four strokes each down and up from the interface. The tubes were then ultracentrifuged at 30,000 rpm for 18 to 20 hours at 5°C. Lipoproteins concentrate in such gradients at layers where the lipoprotein density is equal to the density of the sodium chloride solution. The resulting gradients were partitioned into 8 or 9 cuts, 0.75 cm. each, either by slicing with a tube-slicing device or by aspirating with a finetipped pipette. From the volume of each cut (approximately 1.0 ml.) a 100 X sample was removed for determination of solution density. To the remainder of each cut was added an equal volume of 0.15 M NaCl; each 2ACD (acid citrate-dextrose) is the special anticoagulant solution used when fractionating plasma proteins by the Cohn method (8).8 Citrate-sodium chloride reagent consisted of 0.02 M trisodium citrate and 0.07 M sodium chloride.133

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Protein-Lipid Relationships in Human Plasma. III. In Pregnancy and the Newborn1

Cited by 49 publications

References 15 publications

Thyroxine-Binding by Sera of Pregnant Women, New-Born Infants, and Women With Spontaneous Abortion 1

Thyroxine-Binding by Sera of Pregnant Women, New-Born Infants, and Women With Spontaneous Abortion 1

Protein-Lipid Relationships in Human Plasma: In Biliary Cirrhosis, Obstructive Jaundice, and Acute Hepatitis 1

Lipoproteins in Primary Biliary Cirrhosis 1

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