Quantitative Bestimmung von Aminen und von Aminosäuren als 1-Dimethylamino-naphthalin-5-sulfonsÄureamide auf Dünnschichtchromatogrammen

Seiler, Nikolaus; Wiechmann, Manfred

doi:10.1007/bf00516473

Cited by 125 publications

(10 citation statements)

References 11 publications

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“…They also observed, as have we, that there was a progressive loss of elutable fluorescence after the plates had been removed from the chromatographic solvent. For example, in one experiment with known DNS-glycine and -alanine chromatographed in benzenepyridine-acetic acid (40:10:1, v/v), we obtained the following per cent recovery of fluorescence eluted in methanol-ammonium hydroxide: Since the fluorescence (as measured by thin-layer chromatography scanning) may be regenerated by spraying the plates with triethanolamine-isopropyl alcohol (20:80) (Seiler and Wiechmann, 1966), we suspect that the time-dependent loss of elutable material is related to binding of DNS-amino acid to silica gel as the solvent evaporates. °Values obtained from preparation of five lots of I-8D5 by means of the procedure outlined in Figure 2.…”

Section: Resultsmentioning

confidence: 98%

Isolation and characterization of the clottable low-molecular-weight fibrinogen derived by limited plasmin hydrolysis of human fraction I-4

Sherman¹,

Mosesson²,

Sherry³

1969

Biochemistry

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Section: Resultsmentioning

confidence: 98%

Isolation and characterization of the clottable low-molecular-weight fibrinogen derived by limited plasmin hydrolysis of human fraction I-4

Sherman¹,

Mosesson²,

Sherry³

1969

Biochemistry

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“…Figure 6 represents overlays of the chromatograms, showing separation of various dansylated derivatives of chlorpromazine metabolites in the optimum solvent systems employed in these studies. Estimation of Fluore~cence-The influence of eluting solvents on the quantum yields of fluorescing substances was reported (9,10). From the data in Fig.…”

Section: Resultsmentioning

confidence: 99%

Chlorpromazine Metabolism II: Determination of Nonconjugated Metabolites in Blood of Schizophrenie Patients

Kaul¹,

Conway

Ticku

et al. 1972

Journal of Pharmaceutical Sciences

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“…tern (pH 3) to produce S-mercuric ^-dansylcysteine. Both the $ purity of the starting substance and the products of Hg2+ bind-< ing were evaluated by thin-layer chromatography on Eastman 6060 silica gel plates using a solvent system of chloroformmethanol-acetic acid (75:25:5) (Seiler and Wiechmann, 1967).…”

Section: Methodsmentioning

confidence: 99%

Sulfhydryl-specific fluorescent label, S-mercuric N-dansylcysteine. Titrations of glutathione and muscle proteins

Leavis¹,

Lehrer²

1974

Biochemistry

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A new fluorescent reagent, N-mercuric jV-dansylcysteine, has been developed which can be used both to estimate sulfhydryl groups and to label proteins. The reagent reacts readily with small molecules and proteins to form mercury-bridged mercaptides with extremely high association constants. For small molecules, the fluorescence of the attached label is enhanced 2X without spectral shift, while larger enhancements (2.8, 7, and 20X) and spectral shifts (5, 13, and 20 nm) are observed when the label is bound to the muscle pro-The labeling of proteins with fluorescent molecules has been a useful approach for the study of protein structure and interactions. In order to obtain maximum information from such studies, however, it is desirable to have labels that can be conveniently attached to specific sites on the surface of the protein under conditions which will not alter the protein's native properties.A large number of SH-directed reagents have been developed in recent years which satisfy these requirements to varying degrees. A partial listing of these includes didansylcystine (Wu and Stryer, 1972; Cheung et al., 1971), the iodoacetamide naphthylaminesulfonates (I-AEDANS)1 (Hudson and Weber, 1973), dansylaziridine (Scouten et al., 1974), the fluorescein derivatives: difluorescein cystine (Wu and Stryer, 1972; Bunting and , fluorescein mercuric acetate (Karush et al., 1964; Heitz and Anderson, 1968), and mercurichrome (Weltman et al., 1972); 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-chloride) (Birkett et Allen and Lowe. 1973), N-( 1 -anilinonaphthyl-4)-maleimide (ANM) (Kanaoka et al., 1973), A'-(p-(2-benzimidazolyl)phenyl)maleimide (BIPM) (Kanaoka et al., 1970), and pyrenemaleimide (Weltman et al., 1973).While most of these compounds have proved useful in the study of some protein systems, disadvantages such as limited solubility in aqueous solutions (dansylaziridine, ANM, pyrene-

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Quantitative Bestimmung von Aminen und von Aminosäuren als 1-Dimethylamino-naphthalin-5-sulfonsÄureamide auf Dünnschichtchromatogrammen

Cited by 125 publications

References 11 publications

Isolation and characterization of the clottable low-molecular-weight fibrinogen derived by limited plasmin hydrolysis of human fraction I-4

Isolation and characterization of the clottable low-molecular-weight fibrinogen derived by limited plasmin hydrolysis of human fraction I-4

Chlorpromazine Metabolism II: Determination of Nonconjugated Metabolites in Blood of Schizophrenie Patients

Sulfhydryl-specific fluorescent label, S-mercuric N-dansylcysteine. Titrations of glutathione and muscle proteins

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