Quantitative assessment of platelets, platelet microparticles, and platelet aggregates with flow cytometry

Matzdorff, Axel; ̈hnel, G. Ku; Kemkes‐Matthes, B.; Pralle, H.

doi:10.1016/s0022-2143(98)90059-2

Cited by 41 publications

(41 citation statements)

References 20 publications

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“…Table 4 shows a comparison of whole blood and platelet rich plasma versus platelet poor/free plasma, and single versus double centrifugation. Whole blood and platelet rich plasma have been reported to contain similar numbers of microvesicles (19). Platelet rich plasma (PRP) centrifugation ( 100g 3 10 min) results in 1,000g-min of sedimentation.…”

Section: Centrifugationmentioning

confidence: 99%

Measurement of microvesicle levels in human blood using flow cytometry

Chandler

2016

Cytometry Part B Clinical

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Microvesicles are fragments of cells released when the cells are activated, injured, or apoptotic. Analysis of microvesicle levels in blood has the potential to shed new light on the pathophysiology of many diseases. Flow cytometry is currently the only method that can simultaneously separate true lipid microvesicles from other microparticles in blood, determine the cell of origin and other microvesicle characteristics, and handle large numbers of clinical samples with a reasonable effort, but expanded use of flow cytometric measurement of microvesicle levels as a clinical and research tool requires improved, standardized assays. The goal of this review is to aid investigators in applying current best practices to microvesicle measurements. First pre-analytical factors are evaluated and data summarized for anticoagulant effects, sample transport and centrifugation. Next flow cytometer optimization is reviewed including interference from background in buffers and reagents, accurate microvesicle counting, swarm interference, and other types of coincidence errors, size calibration, and detection limits using light scattering, impedance and fluorescence. Finally current progress on method standardization is discussed and a summary of current best practices provided. V C 2016 Clinical Cytometry Society

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Section: Centrifugationmentioning

confidence: 99%

Measurement of microvesicle levels in human blood using flow cytometry

Chandler

2016

Cytometry Part B Clinical

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“…During this process platelets secrete thin walled vesicles called microparticles or microvesicles (PMP) and smaller exosomes. Under physiological conditions PMP are released after activation with agonists such as epinephrine, adenosine diphosphate, thrombin or collagen [Matzdorff et al, 1998], and also by exposure to complement protein C5b-9 or high shear stress (higher than physiological), [Holme et al, 1997]. In vitro, PMP may be generated by stimulation of platelets with physiological factors like thrombin [George, 1982], thrombin and collagen [Baj-Krzyworzeka et al, 2002], or nonphysiological agonist like calcium ionophore [Forlow et al, 2000].…”

Section: Methods Of Pmp Generation and Their Phenotype Analysis By Flmentioning

confidence: 99%

Application of Flow Cytometry in the Studies of Microparticles

Baj‐Krzyworzeka¹,

Baran²,

Szatanek³

et al. 2012

Flow Cytometry - Recent Perspectives

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“…Current literature showed that flow cytometry has been employed to study platelet function testing in apheresis products, where all the above-mentioned probes were used in conjunction with secondary antibodies. [8] Other studies include flow cytometric analysis of platelet count [13], platelet reactivity [14], platelet function in children [15] and the importance of the sampling site in patient about to undergo surgery [16]; these studies used only one or two of the above-mentioned probes. .…”

Section: Blood Preparation For Flow Cytometrymentioning

confidence: 99%

Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

2013

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In this study, we investigate the validity and laboratory utility of flow cytometry when analyzing platelet activation by studying CD41, CD42b, CD62P and CD63. We compare flow cytometry results from citrated whole blood and finger-prick samples directly after collection and also after storing both a finger-prick and whole blood sample for 24 hours. Citrated whole blood and finger-prick samples were taken from three healthy individuals on two occasions, and a total of 60 000 cells were analyzed for each of the 4 phycoerythrin-labelled monoclonal antibodies. Half of each sample was analyzed immediately after sampling while the other half was kept in the fridge at 6°C for 24 hours before analysis. No significant difference was found between the sampling methods or the period of time before analysis. Results therefore suggest that an appropriately prepared finger-prick sample can be used for platelet function analysis, and samples can be stored for 24 hours in the fridge at 6°C before analysis.

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Quantitative assessment of platelets, platelet microparticles, and platelet aggregates with flow cytometry

Cited by 41 publications

References 20 publications

Measurement of microvesicle levels in human blood using flow cytometry

Measurement of microvesicle levels in human blood using flow cytometry

Application of Flow Cytometry in the Studies of Microparticles

Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

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