2013
DOI: 10.1179/1607845412y.0000000039
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Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

Abstract: In this study, we investigate the validity and laboratory utility of flow cytometry when analyzing platelet activation by studying CD41, CD42b, CD62P and CD63. We compare flow cytometry results from citrated whole blood and finger-prick samples directly after collection and also after storing both a finger-prick and whole blood sample for 24 hours. Citrated whole blood and finger-prick samples were taken from three healthy individuals on two occasions, and a total of 60 000 cells were analyzed for each of the … Show more

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Cited by 3 publications
(2 citation statements)
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“…1 b–e). Whereas CD41 is expressed on all platelets, CD62P is present only on the activated ones 31 , 32 . In blood plasma CD41 + pEVs dominated over other EVs in the order all pEVs (CD41 + ) > mEVs > eEVs > nEVs > activated platelets CD62P + EVs (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…1 b–e). Whereas CD41 is expressed on all platelets, CD62P is present only on the activated ones 31 , 32 . In blood plasma CD41 + pEVs dominated over other EVs in the order all pEVs (CD41 + ) > mEVs > eEVs > nEVs > activated platelets CD62P + EVs (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…CD41 is used as a platelet identifier, as it is only present on platelets and no other circulating blood cells [ 5 ]. P-selectin (CD62P) is a special index of platelet activation that is usually stored in platelet granules and Weibel-Palade bodies of endothelial cells.…”
Section: Introductionmentioning
confidence: 99%