Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

Swanepoel, Albe C.; Stander, André; Pretorius, Etheresia

doi:10.1179/1607845412y.0000000039

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…1 b–e). Whereas CD41 is expressed on all platelets, CD62P is present only on the activated ones 31 , 32 . In blood plasma CD41 + pEVs dominated over other EVs in the order all pEVs (CD41 + ) > mEVs > eEVs > nEVs > activated platelets CD62P + EVs (Fig.…”

Section: Resultsmentioning

confidence: 99%

Large extracellular vesicle (EV) and neutrophil extracellular trap (NET) interaction captured in vivo during systemic inflammation

Ortmann,

Such,

Cichon

et al. 2024

Sci Rep

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Extracellular vesicles (EVs) and neutrophil extracellular traps (NETs) are pivotal bioactive structures involved in various processes including inflammation. Herein we report the interactions between EVs and NETs during murine endotoxemia studied in situ directly in the vasculature (cremaster muscle, liver sinusoids) using intravital microscopy (IVM). We captured NETs and EV release in real time by both non- and polarized neutrophils in liver but not in cremaster vasculature. When comparing numbers of circulating EVs of various origin (nanoparticle tracking analysis—NTA, flow cytometry) with those interacting with endothelium and NETs (IVM) we observed that whereas platelet and monocyte/macrophage-derived EVs dominate in blood and peritoneal lavage, respectively, mostly neutrophil-derived EVs interact with the vascular lining, NETs and leukocytes. Despite the interaction, NETs do not affect EV formation as NET release inhibition did not alter EV release. However, EVs inhibit NETs formation and in particular, erythrocyte-derived EVs downregulate NET release and this effect is mediated via Siglec-E-dependent interactions with neutrophils. Overall, we report that EVs are present in NETs in vivo and they do modulate their release but the process in not bidirectional. Moreover, EVs isolated from body fluids might not reflect their importance in direct endothelial- and leukocyte-related interactions.

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Section: Resultsmentioning

confidence: 99%

Large extracellular vesicle (EV) and neutrophil extracellular trap (NET) interaction captured in vivo during systemic inflammation

Ortmann,

Such,

Cichon

et al. 2024

Sci Rep

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show abstract

“…CD41 is used as a platelet identifier, as it is only present on platelets and no other circulating blood cells [ 5 ]. P-selectin (CD62P) is a special index of platelet activation that is usually stored in platelet granules and Weibel-Palade bodies of endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

CD62P (P-selectin) expression as a platelet activation marker in patients with liver cirrhosis with and without cholestasis

Hegazy¹,

Elsabaawy²,

Eltabakh³

et al. 2021

ceh

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Comparative in vitro evaluation between platelet concentrates from buffy-coat pooling storage in 100% plasma and 80% platelet additive solution SSP+

Dalia¹,

Ludwig

Alvarez

et al. 2021

Int J Blood Transfus Immunohematol

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Aims: To compare the quality of platelet concentrates obtained from buffy-coat stored in pooled plasma during days 1–5, those stored in additive solution during days 1–5 and 7 processed in UMAE HE Puebla Blood Bank, to evaluate the efficacy of platelet additive solutions. Methods: Leukoreduced platelet concentrates (LDPCs) were prepared from pools of four buffy-coats (BCs) suspended in pooled plasma (PP) (March–June 2018); and platelet additive solution (SSP+, Macopharma) (August–October 2019) as a validation process in UMAE HE Blood Bank, Puebla, Mexico. On days 1, 5 (PP), and the 7th day of storage (SSP+), samples were tested for CD45, CD42b, CD62P, and Annexin V (CyAn ADP-Beckman Coulter); pCO2, pO2, and pH (OPTI CCA-TS2); glucose, lactic dehydrogenase (LDH), and Total Proteins (Unicel DXC800-Beckman Coulter); and platelet count and media platelet volume (MPV) (Swilab Alfa—Boule). Results: Platelet recoveries from BC pooling did not present significant differences between PP and SSP+ methods (p >0.05). pH, MPV, and pO2 results were not affected by the storage medium during the storage period. Leukocyte concentration (CD45) complied in both groups with European standards. Glucose consumption and apoptosis markers were of lower values with PP platelets, and activation markers (CD62P) were higher with LDPC in PP (p > 0.05). Conclusion: In vitro analysis of the seven-day storage of LDPC in SSP+ revealed stability in pH with a lower expression of CD62p, among other biochemical markers. Therefore, LDPC in SSP+ is a sustainable product for transfusion with the equivalent quality compared with five-day storage platelets in PP.

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Flow cytometric comparison of platelets from a whole blood and finger-prick sample: impact of 24 hours storage

Cited by 3 publications

References 43 publications

Large extracellular vesicle (EV) and neutrophil extracellular trap (NET) interaction captured in vivo during systemic inflammation

Large extracellular vesicle (EV) and neutrophil extracellular trap (NET) interaction captured in vivo during systemic inflammation

CD62P (P-selectin) expression as a platelet activation marker in patients with liver cirrhosis with and without cholestasis

Comparative in vitro evaluation between platelet concentrates from buffy-coat pooling storage in 100% plasma and 80% platelet additive solution SSP+

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