Comparative in vitro evaluation between platelet concentrates from buffy-coat pooling storage in 100% plasma and 80% platelet additive solution SSP+

Abstract

Aims: To compare the quality of platelet concentrates obtained from buffy-coat stored in pooled plasma during days 1–5, those stored in additive solution during days 1–5 and 7 processed in UMAE HE Puebla Blood Bank, to evaluate the efficacy of platelet additive solutions. Methods: Leukoreduced platelet concentrates (LDPCs) were prepared from pools of four buffy-coats (BCs) suspended in pooled plasma (PP) (March–June 2018); and platelet additive solution (SSP+, Macopharma) (August–October 2019) as a validation process in UMAE HE Blood Bank, Puebla, Mexico. On days 1, 5 (PP), and the 7th day of storage (SSP+), samples were tested for CD45, CD42b, CD62P, and Annexin V (CyAn ADP-Beckman Coulter); pCO2, pO2, and pH (OPTI CCA-TS2); glucose, lactic dehydrogenase (LDH), and Total Proteins (Unicel DXC800-Beckman Coulter); and platelet count and media platelet volume (MPV) (Swilab Alfa—Boule). Results: Platelet recoveries from BC pooling did not present significant differences between PP and SSP+ methods (p >0.05). pH, MPV, and pO2 results were not affected by the storage medium during the storage period. Leukocyte concentration (CD45) complied in both groups with European standards. Glucose consumption and apoptosis markers were of lower values with PP platelets, and activation markers (CD62P) were higher with LDPC in PP (p > 0.05). Conclusion: In vitro analysis of the seven-day storage of LDPC in SSP+ revealed stability in pH with a lower expression of CD62p, among other biochemical markers. Therefore, LDPC in SSP+ is a sustainable product for transfusion with the equivalent quality compared with five-day storage platelets in PP.