Quantitative Assessment of Cervical Neoplasia by Hydrolysed Dna Assay

Sincock, A. M.; Partington, C. K.; Evans-Jones, J.; Steele, S. J.

doi:10.1016/s0140-6736(87)91423-1

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Looking at the hydrolysis curves in our cases, it becomes clear that the increase of hydrolyzed DNA in nuclei from benign urothelium is delayed compared with that of malignant tumor cell nuclei. This has been reported to be a constant finding in various tissues investigated 13–19. In our cases, this early increase was even more pronounced in the more aggressive tumors ( P = 0.001; data not shown).…”

Section: Discussionsupporting

confidence: 76%

“…In contrast to determining DNA ploidy, other studies have investigated the dynamics of the Feulgen hydrolysis reaction, which differentiates a rapidly hydrolyzed component of nuclear DNA, the increase of which is linked to malignancy 13–15. Semiautomated, quantitative determination of acid‐labile DNA has been applied successfully applied to cervical smears to detect cervical intraepithelial neoplasia 16–19. We measured the rapidly hydrolyzable fraction of DNA from patients with normal urothelium and with urothelial tumors to determine whether sufficient discrimination can be achieved between these groups for the purpose of monitoring patients with carcinoma of the bladder and bladder carcinoma screening.…”

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confidence: 99%

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Quantitative assessment of bladder carcinoma by acid labile DNA assay

Gschwendtner

Mairinger

1999

Cancer

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BACKGROUND Effective noninvasive methods for monitoring patients with bladder carcinoma and screening for bladder carcinoma that show better performance than the methods currently in use would be desirable for detecting urothelial carcinoma at an early, easily treatable stage. A rapidly hydrolyzed component of nuclear DNA has been described, the increase of which has been linked to malignancy. Quantitative determination of acid labile DNA has been applied successfully to detect other neoplasms. This study investigates the potential of this method to detect transitional cell carcinomas. METHODS Touch imprints of transurethral resection material from 62 cases of nonmalignant urothelium (control group including reactive changes) and 94 cases of bladder carcinoma were analyzed. The full Feulgen hydrolysis profiles of the nonmalignant and malignant urothelial cells were compared by measuring the staining density of the nuclei using digital image analysis after various hydrolysis times. Twenty cells were sampled randomly from among the cells measured for calculation of the mean optical density (MOD). The MOD of each time was used to build the hydrolysis profile of a case. RESULTS A hydrolysis time of 10 minutes was found to be the most discriminative between control and carcinoma cases. Applying a single threshold MOD value of 101 resulted in a test sensitivity of 95.7%, a specificity of 94.4%, a positive predictive value of 98.3%, a negative predictive value of 95.9%, and an area under the receiver operating characteristic curve of 0.97. CONCLUSIONS The results of this pilot study suggest that measurement of the rapidly hydrolyzed component of DNA present in the nuclei of bladder urothelium offers a highly sensitive and reliable supplement to the qualitative and subjective cytologic procedures currently in use. Cancer 1999;86:105–13. © 1999 American Cancer Society.

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Section: Discussionsupporting

confidence: 76%

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confidence: 99%

Quantitative assessment of bladder carcinoma by acid labile DNA assay

Gschwendtner

Mairinger

1999

Cancer

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“…13 It is important to stress the difference between this study and the numerous ploidy investigations previously CANCER June 15 I99 1 Vol. 67 s t~d i e s .~' Furthermore, in another DNA distribution study, aneuploidy was encountered commonly in severe dysplasias with HPV 16/18.…”

Section: Discussionmentioning

confidence: 73%

“…A pilot study investigating the rapidly hydrolyzable fraction of DNA showed encouraging results in the discrimination of normal from dysplastic lesions of the cervix. 13 It is important to stress the difference between this study and the numerous ploidy investigations previously CANCER June 15 I99 1 Vol. 67 s t~d i e s .~' Furthermore, in another DNA distribution study, aneuploidy was encountered commonly in severe dysplasias with HPV 16/18.…”

Section: Discussionmentioning

confidence: 73%

Quantitative determination of acid-labile dna in cervical intraepithelial neoplasia

Partington

Sincock

Steele

1991

Cancer

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Using a modified Feulgen hydrolysis procedure and integrating microdensitometry, the acid-labile nuclear DNA in exfoliated cervical epithelial cells was quantified in a range of histologically confirmed cervical intraepithelial neoplasia (CIN), invasive cancer, and normal controls. The mean relative optical densities obtained for each sample group showed an increase from normal epithelium, through CIN grades, to invasive cancer. Although there was some overlap between groups, the difference in the overall mean values between the adjacent groups was statistically significant. The sensitivity of the test was 87.1% with a specificity of 99.2% and a predictive value of 99.5%, with no false negatives in the severe dysplasia and cancer groups. Quantitative data allows the threshold value to be altered to vary the sensitivity and specificity according to prevailing requirements. This suggests the possibility of using quantitative acid-labile DNA measurements to improve existing screening for cervical precancer. Cancer 67:3104-3109,1991. HE TRADITIONAL PAPANICOLAOU method of Cyto

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