Quantitative and sensitive RNA based detection of Bacillus spores

Osmekhina, Ekaterina; Shvetsova, Antonina; Ruottinen, Maria; Neubauer, Peter

doi:10.3389/fmicb.2014.00092

Cited by 5 publications

(4 citation statements)

References 64 publications

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“…Overall, compared to reported conventional ELISAs for anthrax spore detection [4,22], PHAD has improved sensitivity, robustness and/or substantially reduced run times. Nucleic acid sequence (DNA or RNA), dipicolinic acid (DPA), and surface resonance (Raman spectroscopy)-based methods of anthrax spore detection all require sample processing procedures (often including sample enrichment) and equipment limiting the portability and practicality in the field [6,23,24,25]. …”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Waller¹,

Hew²,

Holdaway³

et al. 2016

Biosensors

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Portable detection and quantitation methods for Bacillus anthracis (anthrax) spores in pure culture or in environmental samples are lacking. Here, an amperometric immunoassay has been developed utilizing immunomagnetic separation to capture the spores and remove potential interferents from test samples followed by amperometric measurement on a field-portable instrument. Antibody-conjugated magnetic beads and antibody-conjugated glucose oxidase were used in a sandwich format for the capture and detection of target spores. Glucose oxidase activity of spore pellets was measured indirectly via amperometry by applying a bias voltage after incubation with glucose, horseradish peroxidase, and the electron mediator 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid). Target capture was mediated by polyclonal antisera, whereas monoclonal antibodies were used for signal generation. This strategy maximized sensitivity (500 target spores, 5000 cfu/mL), while also providing a good specificity for Bacillus anthracis spores. Minimal signal deviation occurs in the presence of environmental interferents including soil and modified pH conditions, demonstrating the strengths of immunomagnetic separation. The simultaneous incubation of capture and detection antibodies and rapid substrate development (5 min) result in short sample-to-signal times (less than an hour). With attributes comparable or exceeding that of ELISA and LFDs, amperometry is a low-cost, low-weight, and practical method for detecting anthrax spores in the field.

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Section: Discussionmentioning

confidence: 99%

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Waller¹,

Hew²,

Holdaway³

et al. 2016

Biosensors

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show abstract

“…The supernatant was loaded into the microfluidic chips with a 500 µl syringe through a single channel, with a flow rate of 300 µm s −1 . After the CNF fibrils entangled behind the pillars to form a solid transparent membrane, the CNF solution was switched to H 2 O. Phasecontrast and fluorescent images were acquired using an Axio Observer Z1 microscope (Carl Zeiss, Jena, Germany) 45 . Images were acquired every 2 min at ×20 magnification during the experiment.…”

Section: Particle Specificitymentioning

confidence: 99%

Capturing colloidal nano- and microplastics with plant-based nanocellulose networks

et al. 2022

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Microplastics accumulate in various aquatic organisms causing serious health issues, and have raised concerns about human health by entering our food chain. The recovery techniques for the most challenging colloidal fraction are limited, even for analytical purposes. Here we show how a hygroscopic nanocellulose network acts as an ideal capturing material even for the tiniest nanoplastic particles. We reveal that the entrapment of particles from aqueous environment is primarily a result of the network’s hygroscopic nature - a feature which is further intensified with the high surface area of nanocellulose. We broaden the understanding of the mechanism for particle capture by investigating the influence of pH and ionic strength on the adsorption behaviour. We determine the nanoplastic binding mechanisms using surface sensitive methods, and interpret the results with the random sequential adsorption (RSA) model. These findings hold potential for the explicit quantification of the colloidal nano- and microplastics from different aqueous environments, and eventually, provide solutions to collect them directly on-site where they are produced.

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“…After the CNF fibrils entangled behind the pillars to form a solid transparent membrane, the CNF solution was switched to H2O. Phase-contrast and fluorescent images were acquired using an Axio Observer Z1 microscope (Carl Zeiss, Jena, Germany) 40 . Images were acquired every two minutes at 20x magnification during the experiment.…”

Section: Microfluidistic Set-up and Fluorescence Microscopymentioning

confidence: 99%

Capturing the colloidal microplastics with plant-based nanocellulose networks

Leppänen

Lappalainen

Lohtander

et al. 2021

Preprint

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Microplastics accumulate to various aquatic organisms causing serious health issues, and they have raised concerns about human health by entering our food chain. The recovery techniques for the most challenging colloidal fraction even for the analytical purposes are limited. Here we show how hygroscopic nanocellulose network acts as an ideal capturing material even for the tiniest nanoplastic particles. We reveal that the entrapment of particles from the aqueous environment is a result of the network’s hygroscopic nature - a feature which is further intensified with the high surface area. We determine the nanoplastic binding mechanisms using surface sensitive methods, and interpret the results with the random sequential adsorption (RSA) model. The microplastic uptake does not rely on any specific interfacial interaction but rather on the water transport behavior of nanocellulose. These findings hold potential for the explicit quantification of the microplastics from different environments, and eventually, provide solutions to collect those directly on-site where they are produced.

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Quantitative and sensitive RNA based detection of Bacillus spores

Cited by 5 publications

References 64 publications

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Capturing colloidal nano- and microplastics with plant-based nanocellulose networks

Capturing the colloidal microplastics with plant-based nanocellulose networks

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