Quantitative and Reversible Lectin-Induced Association of Gold Nanoparticles Modified with α-Lactosyl-ω-mercapto-poly(ethylene glycol)

Otsuka, Hidenori; Akiyama, Yoshitsugu; Nagasaki, Yukio; Kataoka, Kazunori

doi:10.1021/ja010437m

Cited by 469 publications

(339 citation statements)

References 22 publications

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“…46,47 However, the Au-S linkage is not strong enough under physiological conditions because of the limited oxidative stability of thiolate species, [48][49][50] as well as the exchange reactions with thiolated compounds that occur inside the body. Thiolatemodified surfaces are also damaged by exposure to light, high temperature and oxygen.…”

Section: Synthesis Of End-reactive Peg For Surface Modificationsmentioning

confidence: 99%

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Nagasaki

2011

Polym J

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113

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Non-biofouling surfaces constitute one of the most important subjects in sensitively and selectively detecting biomolecular events. Poly(ethylene glycol) (PEG) chains tethered on substrate surfaces are well known to reduce non-biofouling characteristics. Protein adsorption onto a PEG-chain-tethered surface is strongly influenced by the density of the PEG chain and is almost completely suppressed by the successive treatment of longer PEG chains (5 kDa) followed by the treatment of PEG (2 kDa; mixed-PEG-chain-tethered surface) because of a significant increase in PEG chain density. To modify versatile substrate surface, PEG possessing pentaethylenehexamine at one end (N6-PEG) was prepared via a reductive amination reaction of aldehyde-ended PEG with pentaethylenehexamine. Using N6-PEG, antibody/PEG co-immobilization was conducted on a substrate possessing active ester groups. After the antibody was immobilized on the surface, PEG tethered chains were constructed surrounding the immobilized antibody. It is interesting to note that the PEG-chain-tethered functions not only as a non-fouling agent but also improves immune response. The hybrid surface was also applied to oligo DNA immobilization. The oligo DNA/PEG hybrid surface improved hybridization, retaining its non-fouling ability. Densely packed PEG tethered chains surrounding antibodies and/or oligo DNA improved their orientation on the surface. Thus, this material is promising as a high-performance biointerface for versatile applications. Keywords: antibody; biosensing; DNA; end-functionalized poly(ethylene glycol); enzyme-linked immunosorbent assay; hybrid biointerface; soft interface INTRODUCTION Specific biorecognition on substrate surfaces has long been utilized in many applications such as biosensing, 1 immunodiagnostics, 2 enzyme immunoassay, 3 western blotting 4 and protein microarrays. 5 Important factors for the improvement in specific biorecognition on surfaces are the suppression of nonspecific biofouling and the suitable orientation of immobilized biomolecules. The former decreases nonspecific noise and the background level, and the latter increases the specific recognition level. To improve the non-biofouling character of surfaces, numerous efforts have been undertaken. In general, natural polymers such as albumin, casein and dextran have been used for blocking on substrate surfaces. 6 These treatments work well and suppress protein biofouling extensively. If extremely high performance is required, however, these blocking treatments are not enough. In addition, natural products, especially those derived from animals, may have undesired contents such as bacteria and viruses, which may affect the user. 7 Under these circumstances, synthetic polymers have been recently suggested as suitable surface blocking agents. Numerous types of water-soluble polymers, such as poly(acrylamide), poly(vinyl alcohol), poly(vinyl pyrrolidone) and poly(ethylene glycol) (PEG), have been investigated so far. Recently, new types of synthetic polymers such as poly(methoxy ...

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Section: Synthesis Of End-reactive Peg For Surface Modificationsmentioning

confidence: 99%

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Nagasaki

2011

Polym J

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113

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“…For example, a poly(ethylene) glycol linker has been developed with aldehyde distal to the nanoparticle surface. The aldehyde was functionalized with lactose to create a reversible and specific aggregation sensor sensitive to lectins [59]. The advent of plasmon resonant nanoparticles tunable to the NIR has enabled detection in optically turbid media.…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

2006

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The strong optical absorption and scattering of noble metal nanoparticles is due to an effect called localized surface plasmon resonance, which enables the development of novel biomedical applications. The resonant extinction, which can be tuned to the near-infrared, allows the nanoparticles to act as molecular contrast agents in a spectral region where tissue is relatively transparent. The localized heating due to resonant absorption, also tunable into the near-infared, enables new thermal ablation therapies and drug delivery mechanisms. The sensitivity of these resonances to their environment leads to simple affinity sensors for the detection of low-level molecular analytes. Coupled with their general lack of toxicity, these applications suggest that noble metal nanoparticles are a highly promising class of nanomaterials for new biomedical applications.

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“…Observation of the substrate using a phase-difference optical microscope confirmed the formation of a fine micropattern with features 100 µm in diameter ( figure 4(a)). The PEG-treated region on the patterned substrate repels proteins and, consequently, inhibits cell adhesion [22,23].…”

Section: Resultsmentioning

confidence: 99%

Chondrocyte spheroids on microfabricated PEG hydrogel surface and their noninvasive functional monitoring

Otsuka

Nagamura

Kaneko

et al. 2012

Science and Technology of Advanced Materials

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A two-dimensional microarray of 10 000 (100 × 100) chondrocyte spheroids was constructed with a 100 µm spacing on a micropatterned gold electrode that was coated with poly(ethylene glycol) (PEG) hydrogels. The PEGylated surface as a cytophobic region was regulated by controlling the gel structure through photolithography. In this way, a PEG hydrogel was modulated enough to inhibit outgrowth of chondrocytes from a cell adhering region in the horizontal direction, which is critical for inducing formation of three-dimensional chondrocyte aggregations (spheroids) within 24 h. We further report noninvasive monitoring of the cellular functional change at the cell membrane using a chondrocyte-based field effect transistor. This measurement is based on detection of extracellular potential change induced as a result of the interaction between extracellular matrix protein secreted from spheroid and substrate at the cell membrane. The interface potential change at the cell membrane/gate interface can be monitored during the differentiation of spheroids without any labeling materials. Our measurements of the time evolution of the interface potential provide important information for understanding the uptake kinetics for cellular differentiation.

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Quantitative and Reversible Lectin-Induced Association of Gold Nanoparticles Modified with α-Lactosyl-ω-mercapto-poly(ethylene glycol)

Cited by 469 publications

References 22 publications

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

Chondrocyte spheroids on microfabricated PEG hydrogel surface and their noninvasive functional monitoring

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