Quantitative and qualitative changes in antibiotic resistance genes after passing through treatment processes in municipal wastewater treatment plants

Lee, Jangwoo; Jeon, Jong Hun; Shin, Jingyeong; Jang, Hyun Min; Kim, Sungpyo; Song, Myoung Seok; Kim, Young Mo

doi:10.1016/j.scitotenv.2017.06.250

Cited by 145 publications

(52 citation statements)

References 32 publications

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“…The use of cultureindependent methods to assess bacterial inactivation by UV or UV combined with other disinfection methods is reported by different authors (Becerra-Castro et al, 2016;Sousa et al, 2017;Lee et al, 2017;Zhang et al, 2015;Munir et al, 2011;Di Cesare et al, 2016). In a study conducted in three UWTPs with different UV-disinfection processes (oxidative ditch / UV, activated sludge / UV, membrane biological reactor / UV), as in the present study, Munir Lee et al (2017), using a similar UV dose (25.8 mJ/cm 2 and 27 mJ/cm 2 , respectively) to the one we used (29.7 mJ/cm 2 ) also observed limited effects on the relative abundance of ARGs. The apparent disagreement in the current study between culture-dependent assays, where ~2 log-unit reductions were observed, and cultureindependent measurements, suggest that although viable coliforms might be reduced, in general, the microbiota was not strongly affected by disinfection.…”

Section: Discussionmentioning

confidence: 99%

Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

Narciso-da-Rocha

Rocha

Vaz‐Moreira

et al. 2018

Environment International

109

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Urban wastewater treatment plants (UWTPs) are reservoirs of antibiotic resistance. Wastewater treatment changes the bacterial community and inevitably impacts the fate of antibiotic resistant bacteria and antibiotic resistance genes (ARGs). Some bacterial groups are major carriers of ARGs and hence, their elimination during wastewater treatment may contribute to increasing resistance removal efficiency. This study, conducted at a full-scale UWTP, evaluated variations in the bacterial community and ARGs loads and explored possible associations among them. With that aim, the bacterial community composition (16S rRNA gene Illumina sequencing) and ARGs abundance (real-time PCR) were characterized in samples of raw wastewater (RWW), secondary effluent (sTWW), after UV disinfection (tTWW), and after a period of 3 days storage to monitoring possible bacterial regrowth (tTWW-RE). Culturable enterobacteria were also enumerated. Secondary treatment was associated with the most dramatic bacterial community variations and coincided with reductions of ~2 log-units in the ARGs abundance. In contrast, no significant changes in the bacterial community composition and ARGs abundance were observed after UV disinfection of sTWW. Nevertheless, after UV treatment, viability losses were indicated ~2 log-units reductions of culturable enterobacteria. The analysed ARGs (qnrS, bla, bla, bla, bla, sul1, sul2, and intI1) were strongly correlated with taxa more abundant in RWW than in the other types of water, and which associated with humans and animals, such as members of the families Campylobacteraceae, Comamonadaceae, Aeromonadaceae, Moraxellaceae, and Bacteroidaceae. Further knowledge of the dynamics of the bacterial community during wastewater treatment and its relationship with ARGs variations may contribute with information useful for wastewater treatment optimization, aiming at a more effective resistance control.

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Section: Discussionmentioning

confidence: 99%

Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

Narciso-da-Rocha

Rocha

Vaz‐Moreira

et al. 2018

Environment International

109

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“…Based on protein level analysis, the ermB genes detected were responsible for encoding a protein consisting of 107 amino acids, where similar gene translation patterns were identified. In comparison to other studies, Lee et al [15] detected ermB genes in the effluent of two WWTPs, even after UV application as the disinfection method. Moreover, Wang et al [6] showed the ineffectiveness of WWTPs in the removal of antimicrobial resistant genes, which were prevailing in all samples analyzed.…”

Section: Resultsmentioning

confidence: 73%

Antibiotic Resistance Genes Dynamics at the Different Stages of the Biological Process in a Full-Scale Wastewater Treatment Plant

et al. 2018

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Wastewater treatment plants (WWTPs) highly contribute to the transmission of antibiotic resistance genes (ARGs) in the environment. In this work, the diversity of ermF, ermB, sul1 and int1-enconding genes was examined in the influent, the mixed liquor and the effluent of a full-scale WWTP. Based on the clones analyzed, similar genotypes were recorded at all process stages. However, distinct genotypes of int1 were responsible for the expression of sul1 and ermF genes in Gammaproteobacteria and Bacteroidetes, respectively. Due to the detection of similar ARGs profiles throughout the biological process, it is concluded that additional treatment is needed for their retention.

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“…The removal rates of ARGs and 16S rDNA in WWTP with coag–floc and UV disinfection units also displayed correlation ( p > 0.05). Lee et al () also reported up to 4 log reductions of ARGs for a similarly configurated WWTP. Studies on UV disinfection show that UV treatment alone removes ARGs around 0 log reduction (Lee et al, ; Narciso‐da‐Rocha et al, ).…”

Section: Resultsmentioning

confidence: 87%

“…Lee et al () also reported up to 4 log reductions of ARGs for a similarly configurated WWTP. Studies on UV disinfection show that UV treatment alone removes ARGs around 0 log reduction (Lee et al, ; Narciso‐da‐Rocha et al, ). The removal of ARGs by UV alone demonstrate a positive relationship with the dose of the UV applied (Sharma, Johnson, Cizmas, McDonald, & Kim, ).…”

Section: Resultsmentioning

confidence: 87%

Removal of antibiotic resistance genes in various water resources recovery facilities

Kucukunsal

İçgen

2020

Water Environment Research

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Water resources recovery facilities (WWTPs) are hotspots for antibiotic resistance genes (ARGs) and pose a significant threat to environments. Therefore, ARG removal efficiencies of WWTPs are of great importance. In this study, conventional activated sludge (CAS), biological nutrient removal (BNR), sequencing batch reactor (SBR), membrane bioreactor (MBR), package MBR, and WWTP with coagulation-flocculation and UV disinfection units were investigated in terms of their removal efficiencies on overall bacterial genes with 16S rDNA and seven ARGs including the genes aadA, bla CTX-M , cmlA, ermB, sul1, tetA, and qnrS corresponding to commonly used antibiotics aminoglycosides, β-lactams, amphenicols, macrolides-lincosamides-streptogramins, sulfonamides-trimethoprim, tetracyclines, and quinolones, respectively. Seasonal abundance of overall genes and ARGs in influents and effluents of each WWTPs was determined by quantitative polymerase chain reaction. Membrane bioreactor and package MBR systems showed the highest removal efficiency up to 5-log reductions. Seasonal changes affected ARG removal efficiencies of BNR and CAS significantly (p < 0.05). The lowest log reductions were determined in summer for BNR and in both autumn and winter for CAS. The abundance of the genes increased in sludge treatment of CAS. In all WWTPs tested, ARG removal rates were correlated with the 16S rDNA gene removal (p > 0.05). The results elucidated that the removal mechanism was not ARG-specific. © 2019 Water Environment Federation • Practitioner points• Removal of genes in WWTPs was found to be not ARG-specific. • MBR and package MBR systems had the highest gene removal efficiencies. • Effluents of the MBR systems still retained up to 10 4 gene copy number/ml. • Removal of genes in CAS and BNR systems showed seasonal variations. • The copy numbers of the genes increased in the sludge of CAS.•

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Quantitative and qualitative changes in antibiotic resistance genes after passing through treatment processes in municipal wastewater treatment plants

Cited by 145 publications

References 32 publications

Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

Bacterial lineages putatively associated with the dissemination of antibiotic resistance genes in a full-scale urban wastewater treatment plant

Antibiotic Resistance Genes Dynamics at the Different Stages of the Biological Process in a Full-Scale Wastewater Treatment Plant

Removal of antibiotic resistance genes in various water resources recovery facilities

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