Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis tinctoria Nutt.) by HPLC-DAD and UPLC-ESI-QTOF-MS

Abstract

A simple, accurate and reliable high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD) method was developed and then successfully applied for simultaneous quantitative analysis of eight compounds, including chlorogenic acid (1), (R/S)-flavanomarein (2), butin-7-O-β-D-glucopyranoside (3), isookanin (4), taxifolin (5), 5,7,3 ,5 -tetrahydroxyflavanone-7-O-β-D-glucopyranoside (6), marein (7) and okanin (8), in 23 batches of snow chrysanthemum of different seed provenance and from various habitats. The results showed total contents of the eight compounds in the samples with seed provenance from Keliyang (Xinjiang, China), are higher than in samples from the other five provenances by 52.47%, 15.53%, 19.78%, 21.17% and 5.06%, respectively, which demonstrated that provenance has a great influence on the constituents in snow chrysanthemum. Meanwhile, an ultra performance liquid chromatography coupled with electrospray ionization and quadrupole time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS) was also employed to rapidly separate and identify flavonoids and phenolic acids in snow chrysanthemum from Keliyang. As a result, a total of 30 constituents, including 26 flavonoids and four phenolic acids, were identified or tentatively identified based on the exact mass information, the fragmentation characteristics, and retention times of eight reference standards. This work may provide an efficient approach to comprehensively evaluate the quality of snow chrysanthemum.

Edible flowers as sources of phenolic compounds with bioactive potential

“…Peaks 18d, 20d, and 21d showed similar spectra shapes as the previous ones with λ max around 370-380 nm, and a common MS 2 product ion at m/z at 271 that matched the chalcone butein. The pseudomolecular ion ([M-H] − at m/z 433) of peak 18d was coherent with butein hexosides, being the first of them (the majority one) assigned as coreopsin (butein-4′-glucoside), described as a main flavonoid in other species of the Asteraceae family, such as Coreopsis tinctoria (Chen et al, 2016a;Yang et al, 2016). Peak 20d, possessing a molecular weight 42 u higher that peak 18d, was tentatively assigned as acetylcoreopsin, also reported in Coreopsis tinctoria (Yang et al, 2016).…”

“…The pseudomolecular ion ([M-H] − at m/z 433) of peak 18d was coherent with butein hexosides, being the first of them (the majority one) assigned as coreopsin (butein-4′-glucoside), described as a main flavonoid in other species of the Asteraceae family, such as Coreopsis tinctoria (Chen et al, 2016a;Yang et al, 2016). Peak 20d, possessing a molecular weight 42 u higher that peak 18d, was tentatively assigned as acetylcoreopsin, also reported in Coreopsis tinctoria (Yang et al, 2016). Peak 23d should correspond to a coreopsin derivative, although no definite identity could be assigned to it.…”

Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinase

“…The main active components of the extracts include phenolic acids (chlorogenic acid, caffeic acid) and different flavonoids (flavanomarein, marein, and okanin aurone) 18 . Flavanomarein and marein contents are generally the highest in Coreopsis tinctoria Nutt extracts, and may play a key role in quality assessment 19 . Previous experiments by our group showed that FM was absorbed into the blood as a prototype 20 .…”

UPLC/FT-ICR MS-based high-resolution platform for determining the geographical origins of raw propolis samples

“…Most flavonoids or phenols showed an absorption maximum at around 240-290 or 300-350 nm. However, those phenolic compounds were mostly been detected at a UV wavelength of 280 nm (Seal 2016;Yang et al 2016), so the UPLC-based detection of the phenolic components in EEP samples was done at a wavelength of 280 nm in this study. As shown in Fig.…”