Quantitative Analysis of the Marker Compounds in Salvia miltiorrihiza Root and Its Phytomedicinal Preparations

Yuan, Dan; Pan, Yingni; Fu, Wenwei; Makino, Toshiaki; Kano, Yoshihiro

doi:10.1248/cpb.53.508

Cited by 40 publications

(24 citation statements)

References 25 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CM is a serumfree medium with 24 h incubation with phorbol myristate acetate-treated THP-1 cells. Our previous studies and the other reports showed that the CM might contain proinflammatory cytokines such as tumor necrosis factor-a, IL-1, IL-8, and IL-12; anti-inflammatory cytokines such as transforming growth factor-h, IL-4, IL-10, and IL-13; and IL-6 with both proinflammatory and anti-inflammatory properties (3,11,27,39,40). Previous reports have shown that the imbalance between proinflammatory and anti-inflammatory cytokines can influence neoplastic outcome (3).…”

Section: Discussionmentioning

confidence: 96%

“…The structures of tanshinones and their yield properties in root of S. miltiorrhiza Bunge are shown in Fig. 1 (27). Dexamethasone was purchased from the Sigma.…”

Section: Methodsmentioning

confidence: 99%

“…C, cryptotanshinone. The contents of tanshinones varied from 0.006% to 0.107% for tanshinone I, from 0.056% to 0.558% for tanshinone IIA, and from 0.016% to 0.38% for cryptotanshinone in root of S. miltiorrhiza Bunge (27). migrating into the wound track was evaluated under a phase-contrast microscope and photographed at Â200 magnification.…”

Section: Cell Linesmentioning

confidence: 99%

See 2 more Smart Citations

Anticancer effects of tanshinone I in human non-small cell lung cancer

Lee¹,

Sher

Chen

et al. 2008

Molecular Cancer Therapeutics

116

View full text Add to dashboard Cite

Tanshinones are the major bioactive compounds of Salvia miltiorrhiza Bunge (Danshen) roots, which are used in many therapeutic remedies in Chinese traditional medicine. We investigated the anticancer effects of tanshinones on the highly invasive human lung adenocarcinoma cell line, CL1-5. Tanshinone I significantly inhibited migration, invasion, and gelatinase activity in macrophage-conditioned medium-stimulated CL1-5 cells in vitro and also reduced the tumorigenesis and metastasis in CL1-5-bearing severe combined immunodeficient mice. Unlike tanshinone IIA, which induces cell apoptosis, tanshinone I did not have direct cytotoxicity. Real-time quantitative PCR, luciferase reporter assay, and electrophoretic mobility shift assay revealed that tanshinone I reduces the transcriptional activity of interleukin-8, the angiogenic factor involved in cancer metastasis, by attenuating the DNA-binding activity of activator protein-1 and nuclear factor-KB in conditioned medium-stimulated CL1-5 cells. Microarray and pathway analysis of tumorrelated genes identified the differentially expressed genes responding to tanshinone I, which may be associated with the Ras-mitogen-activated protein kinase and Rac1 signaling pathways. These results suggest that tanshinone I exhibits anticancer effects both in vitro and in vivo and that these effects are mediated at least partly through the interleukin-8, Ras-mitogen-activated protein kinase, and Rac1 signaling pathways. Although tanshinone I has a remarkable anticancer action, its potential anticoagulant effect should be noted and evaluated.

show abstract

Section: Discussionmentioning

confidence: 96%

“…The structures of tanshinones and their yield properties in root of S. miltiorrhiza Bunge are shown in Fig. 1 (27). Dexamethasone was purchased from the Sigma.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Anticancer effects of tanshinone I in human non-small cell lung cancer

Lee¹,

Sher

Chen

et al. 2008

Molecular Cancer Therapeutics

116

View full text Add to dashboard Cite

show abstract

“…Nowadays, this technique has been accepted by WHO as a methodology for the assessment of TCM, and is also taken as a method for quality control of TCM in Chinese Pharmacopoeia [3]. Moreover, it is widely accepted that multiple constituents are responsible for the therapeutic effects of TCM; therefore, to ensure its quality, it is necessary to quantitatively determine the multiple marker compounds simultaneously [4].…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis and chromatographic fingerprinting for the quality evaluation of Forsythia suspensa extract by HPLC coupled with photodiode array detector

Xia

Yang

Wang

et al. 2009

J of Separation Science

View full text Add to dashboard Cite

A simple and reproducible HPLC-photodiode array detector method has been described for evaluating and controlling quality of Forsythia suspensa extract (FSE). First, by analysis of chromatographic fingerprints, the similarities of chromatograms of FSE samples from the same pharmaceutical company exceeded 0.999, 0.997 and 0.960, respectively, although they were much lower from different pharmaceutical companies. Second, by further comparing many batches of extract chromatograph charts with the corresponding reference herb materials, the ''common peaks'' 3, 5, 7 and 10 were defined as ''marker peaks'', which were identified as (1)-pinoresinol-b-D-glucoside, forsythiaside, phillyrin and phillygenin, respectively. Third, four ''marker peaks'' were simultaneously determined based on fingerprint chromatogram for further controlling the quality of FSE quantitatively. Namely, the newly developed method was successfully applied to analyze 38 batches of FSE samples supplied by three pharmaceutical factories, which showed acceptable linearity, intra-day precision (RSDo2.76%), inter-day precision (RSDo3.43%) and the average recovery rates in the range of (95.3872.96)% to (101.6073.08)%. At last, hierarchical clustering analysis and Bayes discriminant analysis statistical methods were used to classify and differentiate the 38 FSE samples to provide the basis for guiding reasonable use of FSE and controlling its quality better.

show abstract

“…Analytical methods for the quantitative determination of the active tanshinones, tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone, in Salvia miltiorrhiza plants and medicinal preparations have been reported (Hu et al, 2005;Shi et al, 2005;Yuan et al, 2005;Liu et al, 2006;Yang et al, 2006;Zhou et al, 2006;Ma et al, 2007). Cryptotanshinone was metabolized to a major metabolite, tanshinone IIA, and trace amounts of several hydroxyl-and dihydroxyl-cryptotanshinone in rats (Li et al, 2005;Song et al, 2005;Hao et al, 2006a;Sun et al, 2007a,b).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma by liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study of a standardized fraction of Salvia miltiorrhiza, PF2401‐SF

Park

Kim

et al. 2008

Biomedical Chromatography

View full text Add to dashboard Cite

A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone, the active components of Salvia miltiorrhiza in rat plasma, was developed. After liquid-liquid extraction with tariquidar as an internal standard, tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone were eluted from an Atlantis dC18 column within 5 min with a mixture of methanol and ammonium formate (10 mm, pH 6.5; 85:15, v/v). The analytes were detected by an electrospray ionization tandem mass spectrometry in the selected reaction monitoring (SRM) mode. The standard curves were linear (r=0.999) over the concentration range of 0.25-80 ng/mL for tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma. The coefficients of variation and the relative errors of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone for intra- and inter-assay at four quality control (QC) concentrations were 1.1-5.1% and -4.0-6.0%, respectively. The lower limit of quantification for tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone was 0.25 ng/mL from 100 microL of plasma. This method was successfully applied to the pharmacokinetic study of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone after oral administration of PF2401-SF, the standardized fraction of Salvia miltiorrhiza enriched with tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone to male Sprague-Dawley rats.

show abstract

Quantitative Analysis of the Marker Compounds in Salvia miltiorrihiza Root and Its Phytomedicinal Preparations

Cited by 40 publications

References 25 publications

Anticancer effects of tanshinone I in human non-small cell lung cancer

Anticancer effects of tanshinone I in human non-small cell lung cancer

Quantitative analysis and chromatographic fingerprinting for the quality evaluation of Forsythia suspensa extract by HPLC coupled with photodiode array detector

Simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma by liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study of a standardized fraction of Salvia miltiorrhiza, PF2401‐SF

Contact Info

Product

Resources

About