Quantitative Analysis of Testicular Histology in Patients with Vas Deferens Obstruction Caused by Childhood Inguinal Herniorrhaphy: Comparison to Vasectomized Men

Matsuda, Tadashi; Hiura, Yoshihito; Muguruma, Koei; Okuno, Hiroshi; Horii, Y; Yoshida, Osamu

doi:10.1016/s0022-5347(01)66450-4

Cited by 28 publications

(9 citation statements)

References 16 publications

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“…The values in maturation arrest group were intermediate lying between the high values of the normal and hypospermatogenesis groups at one side and the low values of SCO group on the other side. These findings proved the intimate association between the morphological parameters of seminiferous tubules and the testicular histopathology similar to other previous studies (Matsuda et al, 1996;Luetjens et al, 2002). This also may reflect the impact of the histopathological insult on morphology of the affected seminiferous tubules.…”

Section: Discussionsupporting

confidence: 91%

Immunohistochemical expression of cyclin A in testicular biopsies of fertile and infertile men: correlation with the morphometry of seminiferous tubules

et al. 2010

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Section: Discussionsupporting

confidence: 91%

Immunohistochemical expression of cyclin A in testicular biopsies of fertile and infertile men: correlation with the morphometry of seminiferous tubules

et al. 2010

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“…52 In cases with vasectomy or herniorraphy it was described to be 12 and 13 Sertoli cells per tubule, respectively. 53 Despite this variation the current paradigm states that the number of Sertoli cells is stable in adult human males but is highly variable between individuals. [24][25][26] The loss of Sertoli cells with aging 27 or possibly after vasectomy 28 might be explained by a disturbed Sertoli-cell proliferation during fetal to peripubertal development.…”

Section: Quantification Of Sertoli and Germ Cell Numbersmentioning

confidence: 99%

Reduced numbers of Sertoli, germ, and spermatogonial stem cells in impaired spermatogenesis

et al. 2011

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A key step in the investigation of male infertility is the appropriate classification of impaired spermatogenesis. In this study, we precisely identified Sertoli and distinct germ-cell types in the rat, the mouse, and in the human testis. As a proof of principle, we studied testis biopsy samples from azoospermic patients with defined spermatogenic defects. Remarkably, we found that already the numbers of Sertoli cells, spermatogonia and a subset of spermatogonia including stem cells are significantly reduced in patients with maturation arrest at the level of primary spermatocytes (n=33) compared with patients with histologically normal spermatogenesis (n=33). In patients with hypospermatogenesis (n=44) a significant reduction of spermatogonial cell numbers was observed. The numbers of primary and diplotene spermatocytes were reduced by 84%. However, the strongest reduction (96%) was revealed in the numbers of spermatids in patients with maturation arrest. In contrast, patients with hypospermatogenesis showed only modestly reduced numbers of spermatocytes and spermatids compared with normal spermatogenesis. No correlation was found with age or obstruction. For a detailed analysis of the patients, we distinguished between 'pool of founder cells'-related deficiencies (reduced numbers of Sertoli cells, spermatogonia, and spermatogonial stem cells) and 'meiotic' deficiencies (reduced numbers of spermatocytes, meiotic divisions, and spermatids). Interestingly, patients with maturation arrest showed meiotic deficiencies (36%), while the majority additionally demonstrated deficiencies in the founder pool (58%). In contrast, patients with normal spermatogenesis most often had no deficiencies at all (45%) or founder pool-related deficiencies (33%) but an apparently normal meiosis. This is the first report showing that many infertile patients face besides meiotic defects the problem of reduced numbers of Sertoli cells, spermatogonia, and spermatogonial stem cells.

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“…Treatment of iatrogenic injuries is usually a challenging problem. Compared with vasectomy reversal, procedures performed after IH repair are more difficult, with lower success rate . Obstruction occurs commonly in inguinal lesion or in the retroperitoneal cavity.…”

Section: Discussionmentioning

confidence: 99%

Laparoscopy‐assisted vasovasostomy for post‐herniorrhaphy vas deferens obstruction

et al. 2020

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Introduction Repair of obstructive azoospermia caused by childhood herniorrhaphy may be difficult. Therefore, intracytoplasmic sperm injection using testicular sperm is performed. However, vasovasostomy combined with laparoscopic surgery is challenging. Case presentation A 42‐year‐old man underwent inguinal hernia repair at age 3. He had normal testicular size, azoospermia, normal hormone levels (follicle‐stimulating hormone, luteinizing hormone, and testosterone), absence of Y chromosome micro deletion, and karyotype:46XY, t(1:21)(p34.1:q22.3). He was diagnosed with obstructive azoospermia. Repeated intracytoplasmic sperm injections using testicular sperm resulted in miscarriages. Vasovasostomy combined with laparoscopic surgery was subsequently performed. Postoperative semen analysis result was almost normal. After intracytoplasmic sperm injection of ejaculated sperm, his wife got pregnant. Conclusion Even if patients have chromosomal abnormalities, performing microsurgical re‐anastomosis first is recommended. To our knowledge, this is the first case of a laparoscopy‐assisted vasovasostomy for post‐herniorrhaphy vas deferens obstruction in Japan.

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Quantitative Analysis of Testicular Histology in Patients with Vas Deferens Obstruction Caused by Childhood Inguinal Herniorrhaphy: Comparison to Vasectomized Men

Abstract: Patients with vas deferens obstruction caused by childhood inguinal herniorrhaphy have decreased spermatogenesis compared to post-vasectomy patients, probably due to the longer obstruction period.

Cited by 28 publications

References 16 publications

Immunohistochemical expression of cyclin A in testicular biopsies of fertile and infertile men: correlation with the morphometry of seminiferous tubules

Immunohistochemical expression of cyclin A in testicular biopsies of fertile and infertile men: correlation with the morphometry of seminiferous tubules

Reduced numbers of Sertoli, germ, and spermatogonial stem cells in impaired spermatogenesis

Laparoscopy‐assisted vasovasostomy for post‐herniorrhaphy vas deferens obstruction

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