Quantitative analysis of subretinal injections in the rat

Abstract

The retinal surface affected by a single subretinal injection increases with the injected volume, but this increase is not proportional. Higher volumes lead to a loss of injected solution, either in the vitreous body or through the sclerotomy. In 2-month-old rats, a 3-microl subretinal injection appears to have the best reproducibility, with 20-30% of retinal surface covered by the injected dye.

Gene delivery to the rat retina by non-viral vectors based on chloroquine-containing cationic niosomes

“…It is a less invasive route, more easily to perform, and higher doses can be delivered [46]. Consequently, large retinal surfaces can be transfected by this route of administration [47]. When we administered 4 μl of DPP80-CQ nioplexes by intravitreal injection, the inner layers of the retina (GCL and INL) were mainly transfected as observed in Fig.…”

In vivo retinotopic mapping of superior colliculus using manganese-enhanced magnetic resonance imaging

“…Recent reports using Mn-chelates such as the FDA-approved Mn-DPDP have provided promising results as a less toxic alternative to MnCl 2 for MEMRI studies of optic nerve tract tracing (Olsen et al, 2008), retinal functions (Tofts et al, 2010) and neonatal hypoxic-ischemic cerebral injury (Yang and Wu, 2009), and are potentially applicable to study brain topological organization and diagnosis in the future. Conceptually, we may also improve the precision of in vivo retinotopic mapping by producing more localized retinal lesions with a laser ophthalmoscope Cowey, 1983, 1987) or by more focal injection of contrast agents into the retina (Dureau et al, 2000;Plas et al, 2005). For repeated mapping of different retinotopic subregions in the same subjects, future experiments may also employ activity-induced manganese-dependent MRI (AIM-MRI) (Aoki et al, 2002;Yu et al, 2005) by visual stimulations to different local visual fields without lesioning the visual pathway.…”

BDNF-treated retinal progenitor sheets transplanted to degenerate rats: Improved restoration of visual function

“…A maximum of 8 ng BDNF was administered into the subretinal space and around 2000-fold less BDNF was measured 24 h after an in vitro incubation test. The maximum volume that can be injected reliably into the subretinal space is about 3 ml (Dureau et al, 2000); thus, the total volume of the subretinal space is likely only about 5 ml. However, the microspheres and the transplant were confined to a small area (about 1 mm 2 ) so the local concentration of BDNF could be much higher.…”