Gene delivery to the rat retina by non-viral vectors based on chloroquine-containing cationic niosomes

Mashal, Mohamed; Attia, Noha; Martı́nez, Gema; Soto-Sánchez, Cristina; Fernández, Eduardo; Grijalvo, Santiago; Eritja, Ramón; Puras, Gustavo; Pedraz, José Luís

doi:10.1016/j.jconrel.2019.05.010

Cited by 39 publications

(27 citation statements)

References 50 publications

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“…Compared with non-viral vectors, the transfection rate and immunogenicity of the viral vector are both higher, but the vector is more toxic, the capacity of the target gene is smaller, the targeting specificity is worse, the preparation is more complicated, and the cost is higher. Compared with viral vectors, non-viral vectors feature an ease of production, high yield, and low cost, and they can be widely used for drug delivery [174][175][176][177][178]. Chitosan derivative nanoparticles, as non-viral vectors, have excellent solubility, biodegradability, biocompatibility, non-toxicity, and a higher transfection rate than chitosan nanoparticles [73,98,179].…”

Section: Chitosan Derivative Nanoparticles For the Delivery Of Genementioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

503

259

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Chitosan is a product of the deacetylation of chitin, which is widely found in nature. Chitosan is insoluble in water and most organic solvents, which seriously limits both its application scope and applicable fields. However, chitosan contains active functional groups that are liable to chemical reactions; thus, chitosan derivatives can be obtained through the chemical modification of chitosan. The modification of chitosan has been an important aspect of chitosan research, showing a better solubility, pH-sensitive targeting, an increased number of delivery systems, etc. This review summarizes the modification of chitosan by acylation, carboxylation, alkylation, and quaternization in order to improve the water solubility, pH sensitivity, and the targeting of chitosan derivatives. The applications of chitosan derivatives in the antibacterial, sustained slowly release, targeting, and delivery system fields are also described. Chitosan derivatives will have a large impact and show potential in biomedicine for the development of drugs in future.

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Section: Chitosan Derivative Nanoparticles For the Delivery Of Genementioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

503

259

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“…[ 51a ] Additionally, incorporation of endosomolytic agents such as chloroquine into cationic nanoparticles can improve the cytosolic release of nucleic acids. [ 52 ]…”

Section: Cytosolic Delivery Via Endosomal Escapementioning

confidence: 99%

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Xing

Wang

et al. 2020

Adv Funct Materials

103

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Biopharmaceuticals have emerged to play a vital role in disease treatment and have shown promise in the rapidly expanding pharmaceutical market due to their high specificity and potency. However, the delivery of these biologics is hindered by various physiological barriers, owing primarily to the poor cell membrane permeability, low stability, and increased size of biologic agents. Since many biological drugs are intended to function by interacting with intracellular targets, their delivery to intracellular targets is of high relevance. In this review, the authors summarize and discuss the use of nanocarriers for intracellular delivery of biopharmaceuticals via endosomal escape and, especially, the routes of direct cytosolic delivery by means including the caveolae‐mediated pathway, contact release, intermembrane transfer, membrane fusion, direct translocation, and membrane disruption. Strategies with high potential for translation are highlighted. Finally, the authors conclude with the clinical translation of promising carriers and future perspectives.

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“…For that purpose, specific fluorescent endocytic markers such as dextrans, cholera toxin B, or transferrin can be used to stain the most representative endocytosis pathways (macropinocitosis, caveolae, and clathrin-mediated endocytosis, respectively). The colocalization of such dyes with fluorescent labelled niosomes, or preferably, fluorescent plasmids attached on the surface of niosomes can be qualitatively evaluated by confocal microscopy [91], or quantified by different overlay coefficients, such as Mander´s or Pearson´s colocalization coefficients [9,114] ( Figure 5). Additionally, intracellular trafficking studies can be completed with lysosome markers such as lysotrackers [90] or with different uptake inhibitors such as genistein, wortmannin, or chlorpromazine, to inhibit selectively caveolae, macropinocytosis, or clathrin-mediated endocytosis, respectively [115].…”

Section: In Vitro Biological Evaluation Of Niosomes For Gene Deliverymentioning

confidence: 99%

“…Retinal cross sections micrographs obtained by confocal microscopy (A1,B1), confocal fluorescence micrographs of whole mount (A2,B2). Adapted with permission from Mashal et al [114]. Copyright 2019, Elsevier B.V.…”

Section: Niosomes For Gene Delivery To the Retinamentioning

confidence: 99%

“…Consequently, during the last years, research on non-viral vectors has gained "momentum" as a safer alternative to their viral vectors counterparts, and the number of clinical trials has considerably increased since 2010 (www.clinicaltrials.gov). In 2018, another niosome formulation elaborated with a commercially available DOTMA cationic lipid, lycopene as a "helper" lipid and polysorbate 60 as a non-ionic surfactant, exhibited high levels of protein expression in both primary cortical cultures of rat embryos and in in vivo conditions after intracranial injection in the cortex [114]. Such niosomes were characterized in terms of size, superficial charge, polydispersity, or capacity to protect genetic material against enzymatic digestion in an agarose gel electrophoresis assay.…”

Section: Future Perspectivesmentioning

confidence: 99%

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Niosome-Based Approach for In Situ Gene Delivery to Retina and Brain Cortex as Immune-Privileged Tissues

et al. 2020

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Non-viral vectors have emerged as a promising alternative to viral gene delivery systems due to their safer profile. Among non-viral vectors, recently, niosomes have shown favorable properties for gene delivery, including low toxicity, high stability, and easy production. The three main components of niosome formulations include a cationic lipid that is responsible for the electrostatic interactions with the negatively charged genetic material, a non-ionic surfactant that enhances the long-term stability of the niosome, and a helper component that can be added to improve its physicochemical properties and biological performance. This review is aimed at providing recent information about niosome-based non-viral vectors for gene delivery purposes. Specially, we will discuss the composition, preparation methods, physicochemical properties, and biological evaluation of niosomes and corresponding nioplexes that result from the addition of the genetic material onto their cationic surface. Next, we will focus on the in situ application of such niosomes to deliver the genetic material into immune-privileged tissues such as the brain cortex and the retina. Finally, as future perspectives, non-invasive administration routes and different targeting strategies will be discussed.

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Gene delivery to the rat retina by non-viral vectors based on chloroquine-containing cationic niosomes

Cited by 39 publications

References 50 publications

Chitosan Derivatives and Their Application in Biomedicine

Chitosan Derivatives and Their Application in Biomedicine

Nanocarrier‐Mediated Cytosolic Delivery of Biopharmaceuticals

Niosome-Based Approach for In Situ Gene Delivery to Retina and Brain Cortex as Immune-Privileged Tissues

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