Quantitative analysis of psilocybin and psilocin and psilocybe baecystis (singer and smith) by high-performance liquid chromatography and by thin-layer chromatography

Beug, Michael W.; Bigwood, Jeremy

doi:10.1016/s0021-9673(00)88741-5

Cited by 46 publications

(33 citation statements)

References 7 publications

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“…UV Stamets et al, 1980, Beug and Bigwood, 1981 Methanol with ammonium nitrate, mixing twice for 30 min.…”

Section: Uv Fldmentioning

confidence: 99%

“…** DMAB = 4-(dimethylamino)-benzaldehyde, FID = flame ionization detection, MS = mass spectrometry, UV = ultra violet spectrophotometric detection, VIS = visible spectrophotometry, FLD = fluorescence spectrophotometric detection, ED = electrochemical detection. Beug and Bigwood (1981) obtained quantitative extraction of psilocybin and psilocin from powdered freeze-dried mushrooms by magnetic stirring for 12 hours in methanol. Recoveries were tested by adding known amounts of psilocin and psilocybin to Psathyrella foenisecii and Psathyrella baeocystis.…”

Section: Msmentioning

confidence: 99%

“…Other solvent systems that have been used for these purposes are n-butanol-acetic acid-water (12:3:5) and n-butanol-acetic acid-water-isopropanol (8:2:5:3) (Michaelis, 1977). Heim et al, 1966b;Høiland , 1978;Hatfield and Valdes, 1978;White, 1979;Beug and Bigwood, 1981;Gartz, 1985c n-Butanol-acetic acid-water (2:1:1) Stamets et al, 1980;Bigwood, 1981, 1982;Stijve et al, 1984, Marcano et al, 1994 n-Butanol-acetic acid-water (24:10:10) SG 0.19 0.50-0.54 n.r. Picker and Rickards, 1970;Wurst et al, 1984;Semerdžieva et al, 1986;Wurst et al, 1992. n-Butanol-acetic acid-isopropanol-water 0.22 Leslie, 1977a, 1977b;Repke et al, 1977, Hatfield andValdes, 1978;Koike et al, 1981 n-Propanol-6% ammonia n.r.…”

Section: Qualitative Analysis Of Psilocybin and Psilocin In Mushroom mentioning

confidence: 99%

“…Picker and Rickards, 1970;Wurst et al, 1984;Semerdžieva et al, 1986;Wurst et al, 1992. n-Butanol-acetic acid-isopropanol-water 0.22 Leslie, 1977a, 1977b;Repke et al, 1977, Hatfield andValdes, 1978;Koike et al, 1981 n-Propanol-6% ammonia n.r. Beug and Bigwood, 1981 The most commonly used reagent to detect indole alkaloides on paper or thin-layer chromatograms is 4-(dimethylamino)-benzaldehyde (DMAB). It is usually applied in a mixture with strong hydrochloric acid (Ehrlich´s reagent) or followed by exposition to hydrogen chloride fumes.…”

Section: Qualitative Analysis Of Psilocybin and Psilocin In Mushroom mentioning

confidence: 99%

“…Methanolic extracts were stable for over a year at -5 o C, but lost all psilocin and some psilocybin within six months when stored at room temperature. Some dried herbarium material had lost all psilocybin and psilocin after 1 year (Beug and Bigwood, 1981).…”

Section: Influence Of Cultivation Storage and Processingmentioning

confidence: 99%

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Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Andersson¹,

Gry²,

Kristinsson³

2009

TemaNord

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“…UV Stamets et al, 1980, Beug and Bigwood, 1981 Methanol with ammonium nitrate, mixing twice for 30 min.…”

Section: Uv Fldmentioning

confidence: 99%

Section: Msmentioning

confidence: 99%

Section: Qualitative Analysis Of Psilocybin and Psilocin In Mushroom mentioning

confidence: 99%

Section: Qualitative Analysis Of Psilocybin and Psilocin In Mushroom mentioning

confidence: 99%

Section: Influence Of Cultivation Storage and Processingmentioning

confidence: 99%

See 3 more Smart Citations

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Andersson¹,

Gry²,

Kristinsson³

2009

TemaNord

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Psilocybin and Hallucinogenic Mushrooms

Barceloux

2012

Medical Toxicology of Drug Abuse

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Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis

Gotvaldová

Hájková

Borovička

et al. 2020

Drug Testing and Analysis

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Psilocybin, psilocin, baeocystin, norbaeocystin, and aeruginascin are tryptamines structurally similar to the neurotransmitter serotonin. Psilocybin and its pharmacologically active metabolite psilocin in particular are known for their psychoactive effects. These substances typically occur in most species of the genus Psilocybe (Fungi, Strophariaceae). Even the sclerotia of some of these fungi known as “magic truffles” are of growing interest in microdosing due to them improving cognitive function studies. In addition to microdosing studies, psilocybin has also been applied in clinical studies, but only its pure form has been administrated so far. Moreover, the determination of tryptamine alkaloids is used in forensic analysis. In this study, freshly cultivated fruit bodies of Psilocybe cubensis were used for monitoring stability (including storage and processing conditions of fruiting bodies). Furthermore, mycelium and the individual parts of the fruiting bodies (caps, stipes, and basidiospores) were also examined. The concentration of tryptamines in final extracts was analyzed using ultra‐high‐performance liquid chromatography coupled with mass spectrometry. No tryptamines were detected in the basidiospores, and only psilocin was present at 0.47 wt.% in the mycelium. The stipes contained approximately half the amount of tryptamine alkaloids (0.52 wt.%) than the caps (1.03 wt.%); however, these results were not statistically significant, as the concentration of tryptamines in individual fruiting bodies is highly variable. The storage conditions showed that the highest degradation of tryptamines was seen in fresh mushrooms stored at −80°C, and the lowest decay was seen in dried biomass stored in the dark at room temperature.

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Quantitative analysis of psilocybin and psilocin and psilocybe baecystis (singer and smith) by high-performance liquid chromatography and by thin-layer chromatography

Cited by 46 publications

References 7 publications

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Psilocybin and Hallucinogenic Mushrooms

Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis

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