Quantitative analysis of metronidazole in intravenous admixture with ciprofloxacin by first derivative spectrophotometry

Vega, Elena María; Solá, Nancy Haydée

doi:10.1016/s0731-7085(00)00596-3

Cited by 40 publications

(19 citation statements)

References 13 publications

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“…Various methods such as; spectrophotometry, 5) atomic absorption spectrometry, 6) ion selective electrodes, 7) fluorimetric method, 8) near i n f r a r ed r e f l e c t a n c e s p e c t r o m e t r y, 9 ) a n d chromatography 2) have been used for the determination of RT and MT. However, lack of selectivity has restricted the application of these methods for pharmacokinetic studies in pharmaceutical and biological samples.…”

Section: 2)mentioning

confidence: 99%

Simultaneous Determination of Ranitidine and Metronidazole at Poly(thionine) Modified Anodized Glassy Carbon Electrode

Rahman¹,

Li²,

Jeon³

et al. 2012

Journal of Electrochemical Science and Technology

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:A simple and sensitive electrochemical sensor for simultaneous and quantitative detection of ranitidine (RT) and metronidazole (MT) was developed, based on a poly(thionine)-modified anodized glassy carbon electrode (PTH/GCE). The modified electrode showed the excellent electrocatalytic activity towards the reduction of both RT and MT in 0.1 M phosphate buffer solution (PBS, pH 7.0). The peak-to-peak separations (∆E p ) for the simultaneous detection of RT and MT between the two reduction waves in CV and DPV were increased significantly from ca. 100 mV at anodized GCE, to ca. 550 mV at the PTH/GCE. The reduction peak currents of RT and MT were linear over the range from 35 to 500 µM in the presence of 200 and 150 µM of RT and MT, respectively. The sensor showed the sensitivity of 0.58 and 0.78 µA/cm 2 /µM with the detection limits (S/N = 3) of 1.5 and 0.96 µM, respectively for RT and MT.

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Section: 2)mentioning

confidence: 99%

Simultaneous Determination of Ranitidine and Metronidazole at Poly(thionine) Modified Anodized Glassy Carbon Electrode

Rahman¹,

Li²,

Jeon³

et al. 2012

Journal of Electrochemical Science and Technology

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“…Due to the critical roles of ranitidine and metronidazole in the pharmaceutical industry, their determination is important in biological fluids and drug formulations. Different methods such as; spectrophotometry [8,9], atomic absorption spectrometry [10], ion selective electrodes [11], fluorimetric method [12], near infrared reflectance spectrometry [13] have been used for the determination of ranitidine and metronidazole. These methods, however, are not adaptable for use in pharmacokinetic studies because of their lack of selectivity, due to different interference exist in pharmaceutical samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Ranitidine and Metronidazole at Glassy Carbon Electrode Modified with Single Wall Carbon Nanotubes

et al. 2007

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Single-walled carbon nanotubes(SWCNTs) were dispersed into DMSO, and a SWCNTs-film coated glassy carbon electrode was achieved via evaporating the solvent. The results indicated that CNT modified glassy carbon electrode exhibited efficiently electrocatalytic reduction for ranitidine and metronidazole with relatively high sensitivity, stability and life time. Under conditions of cyclic voltammetry, the potential for reduction of selected analytes is lowered by approximately 150 mV and current is enhanced significantly (7 times) in comparison to the bare glassy carbon electrode. The electrocatalytic behavior is further exploited as a sensitive detection scheme for these analytes determinations by hydrodynamic amperometry. Under optimized condition in amperometric method the concentration calibration range, detection limit and sensitivity were about, 0.1 -200 mM, detection limit (S/N ¼ 3) 6.3 Â 10 À8 mol L À1 and sensitivity 40 nA/mM for metronidazole and 0.3 -270 mM 7.73 Â 10 À8 mol L À1 and 25 nA/mM for ranitidine. In addition, the ability of the modified electrode for simultaneous determination of ranitidine and metronidazole was evaluated. The proposed method was successfully applied to ranitidine and metronidazole determination in tablets. The analytical performance of this sensor has been evaluated for detection of these analytes in serum as a real sample.

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“…MET has been determined by several methods involving spectrophotometry [23][24][25][26] and HPLC. [27][28][29][30][31][32] MET in intravenous admixture with CIP was determined by first-derivative spectrophotometry 33) and LC. 34) Literature reports only one article for the simultaneous determination of CIP and MET in tablet form by the same authors of the present work.…”

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confidence: 99%

Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Mahrouse

Elkady

2011

CHEMICAL & PHARMACEUTICAL BULLETIN

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Regular ArticleCiprofloxacin (CIP, Fig. 1a) [1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(piperazinyl)-quinolone-3-carboxylic acid] belongs to the second generation of fluoroquinolone antibacterial agents. These quinolones are effective against Grampositive and Gram-negative bacteria through inhibiting bacterial DNA gyrase (topoisomerase), the enzyme responsible for maintaining the superhelical twists in the bacterial DNA, leading to inhibition of bacterial DNA synthesis.1) Similar to other fluoroquinolones, CIP has shown clinical efficacy in the treatment of a wide range of systemic infections such as urinary tract, respiratory, gastrointestinal and cutaneous infections. Because of its low toxicity, very wide spectrum of antibacterial effect and low ability to cause bacterial resistance, CIP has been widely used in clinical practice.2) Metronidazole (MET, Fig. 1b) is used as an antiprotozoal, antiamebic and antibacterial drug.3) It is chemically designated as 2-methyl-5-nitroimidazole-1-ethanol. Since CIP has a reduced activity against anaerobic pathogens, CIP and MET are coformulated together in pharmaceutical dosage forms for the treatment of mixed aerobic/anaerobic infections. 4)A survey of literature has revealed several analytical methods for the determination of CIP in pharmaceutical dosage form and biological fluids, including spectrophotometry, [5][6][7][8] spectrofluorimetry, 9-11) HPLC, 12-16) capillary electrophoresis 17,18) and HPTLC. 19) CIP in admixtures with MET and ampicillin has been determined by NMR. 20) Recently, HPLC methods either with fluorescence detection or coupled with mass spectrometry (LC/MS) for determination of ciprofloxacin in human plasma 21,22) have also been published. MET has been determined by several methods involving spectrophotometry [23][24][25][26] and HPLC. [27][28][29][30][31][32] MET in intravenous admixture with CIP was determined by first-derivative spectrophotometry 33) and LC. 34) Literature reports only one article for the simultaneous determination of CIP and MET in tablet form by the same authors of the present work. In this article, a reversed phase ion-pair HPLC and TLC densitometry have been adopted for the simultaneous determination of both drugs in tablets.35) However, due to lack of such equipments in many resources-limited countries and the high costs of HPLC grade solvents and columns, alternative methods are needed to facilitate and increase the speed of analysis, with relatively few costs. To the best of the authors' knowledge, no previous article concerning simultaneous spectrophotometric determination of the two drugs was published. Spectrophotometry continues to be very popular, because of its simplicity, versatility and low cost. Therefore, the aim of the present work was to develop and validate different UV spectrophotometric and chemometric techniques for the simultaneous determination of CIP and MET in tablets without previous separation. Both derivative and derivative ratio spectrophotometry are useful technique for resolving two overlapping...

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Quantitative analysis of metronidazole in intravenous admixture with ciprofloxacin by first derivative spectrophotometry

Cited by 40 publications

References 13 publications

Simultaneous Determination of Ranitidine and Metronidazole at Poly(thionine) Modified Anodized Glassy Carbon Electrode

Simultaneous Determination of Ranitidine and Metronidazole at Poly(thionine) Modified Anodized Glassy Carbon Electrode

Simultaneous Determination of Ranitidine and Metronidazole at Glassy Carbon Electrode Modified with Single Wall Carbon Nanotubes

Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

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