Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies

Sritularak, Boonchoo; Morinaga, Osamu; Yuan, Chun Su; Shoyama, Yukihiro; Tanaka, Hiroyuki

doi:10.1007/s11418-009-0332-x

Cited by 27 publications

(23 citation statements)

References 16 publications

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“…ELISA combines the advantages of the high specificity of antibodies and the great sensitivity of simple enzyme assays. Tanaka et al 114,208. developed a new immuno-staining ELISA method called Eastern blotting.…”

Section: Analytical Advancesmentioning

confidence: 99%

Isolation and analysis of ginseng: advances and challenges

2011

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Ginseng occupies a prominent position in the list of best-selling natural products in the world. Because of its complex constituents, multidisciplinary techniques are needed to validate the analytical methods that support ginseng's use worldwide. In the past decade, rapid development of technology has advanced many aspects of ginseng research. The aim of this review is to illustrate the recent advances in the isolation and analysis of ginseng, and to highlight their new applications and challenges. Emphasis is placed on recent trends and emerging techniques. The current article reviews

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Section: Analytical Advancesmentioning

confidence: 99%

Isolation and analysis of ginseng: advances and challenges

2011

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“…Furthermore, the GC-KO extract was prepared from LE using an anti-GC mAb-coupled immunoaffinity column. Our laboratory has previously prepared mAbs against natural bioactive compounds such as ginsenosides [5,54,55], berberine [10], crocin [2], sennosides [12], and forskolin [1]. Moreover, the immunoaffinity columns coupled with anti-G-Rb 1 [17], anti-forskolin [15], anti-solasodine glycoside [16], and anti-GC mAbs [18] achieved one-step purification of target compounds from crude extracts.…”

Section: Discussionmentioning

confidence: 99%

Preparation of Knockout Extract by Immunoaffinity Column and Its Application

Uto¹,

Tung²,

Morinaga³

et al. 2012

Antibodies

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Importance of herbal medicines have recently increased owing to rising interest in their health benefits. However, medicinal plant extracts are complex mixtures of phytochemicals that act synergistically or additively on specific and/or multiple molecular and cellular targets. Thus, it is difficult to examine the actual pharmacological roles of active compounds in plant extracts. This review describes a new strategy for isolating target compounds from plant extracts using immunoaffinity columns coupled with monoclonal antibodies (mAbs) against natural compounds. Through one-step purification using mAb-coupled immunoaffinity columns, we succeeded in preparing a knockout (KO) extract, which contains all components except the target compound. Furthermore, we investigated the pharmacological effects of the KO extract to reveal the actual effects of a bioactive compound in the crude extract. This approach may help determine the potential function of target compounds in herbal medicines

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“…The ginseng berry has many unique bioactive constituents compared to the generally used ginseng root. Through various assays [13,[119][120][121][122] …”

Section: Exploring Other Bioactive Constituents In the Ginseng Berry mentioning

confidence: 99%

“…The ginseng berry has many unique bioactive constituents compared to the generally used ginseng root. Through various assays [13,[119][120][121][122], a new triterpenoid saponin, isoginsenoside-Rh3 [123], alkaloid ginsenine [124] and a dammarane-type triterpene ketone, panaxadione [125,126], were isolated. Interestingly, ginsenoside-free molecules promote ethanol metabolism [127].…”

Section: Exploring Other Bioactive Constituents In the Ginseng Berry mentioning

confidence: 99%

Ginseng Berry, a Promising Anti-Aging Strategy: Recent Opinions on the Biological Effects of a Traditional Korean Ingredient

Seo¹

2016

JARB

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Journal of Advanced Research in Biotechnology Open Access Review Article Introduction: Ginseng and the Ginseng berryGinseng (Panax ginseng) is a popular herbal medicine that has been used in Asia for 5,000 years [1]. Ginseng is classified as fresh, white, or red ginseng, depending on the processing method. As a traditional herb, red ginseng is known as an adaptogen that restores and improves normal well-being. The use of this herbal plant has been widespread throughout the world because of its therapeutic effects. The well-known biochemical and pharmacological effects include anti-cancer [2], anti-fatigue [3], and anti-diabetic effects [4], along with promoting the synthesis of DNA, RNA and proteins [5]. The herb is used as a tea, an extract, or raw directly from powdered root [6]. The representative bioactive compounds are widely considered to begin senosides, which are ginseng-specific saponins [7]. Currently, more than 100 naturally occurring saponins of various types and products of enzymatic conversion have been isolated from the roots, stems, leaves, flowers, berries, and seeds of ginseng. The different parts of ginseng contain distinct ginsenoside profiles, and thus, different parts probably possess different pharmacological effects [8].Recently, many health reports have recommended an increase in fruit intake as part of a healthy dietary pattern [9,10]. These reports allow for various forms of fruit, including fresh, frozen, and dried, as well as juices, and recommend fruits such as oranges, apples, bananas, grapes, raisins, and berries. Whereas berries are known as a good source of potassium or fiber, recent studies suggest that berry fruits are a rich source of many phytochemicals that have a broad spectrum of bioactivity and a positive impact on general health. Several berry fruits, including blackberries, blueberries, cranberries, raspberries, and strawberries, have recently received attention as a result of their effects in vitro and their associations with lowered risks for some chronic diseases, which were found in recent observational research [11,12]. As a perennial herb, ginseng develops flowers and fruits that bloom in its third and fourth year. Unlike the widely used ginseng root, the ginseng berry is preserved for planting and has not been used by general populations. A recent study reported that the ginseng leaf and berry have higher levels of ginsenosides than ginseng root, and their pharmacological activities have also been reported [13]. In this review, we will summarize the research on the role of dietary ginseng berries in delaying aging, as well as evidence suggesting positive biological effects to prevent age-related diseases. Ginseng Berry Bioactive Content and CompositionThe bioactive components of ginseng are triterpene glycosides or saponins, which are commonly regarded as ginsenosides. AbstractA recent effort in the development of new medications and immune modulatory agents is to search for candidates among natural products because they have relatively low toxicities in clinica...

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Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies

Cited by 27 publications

References 16 publications

Isolation and analysis of ginseng: advances and challenges

Isolation and analysis of ginseng: advances and challenges

Preparation of Knockout Extract by Immunoaffinity Column and Its Application

Ginseng Berry, a Promising Anti-Aging Strategy: Recent Opinions on the Biological Effects of a Traditional Korean Ingredient

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