Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by high-performance liquid chromatography with photo-diode array and mass spectrometric detection

Justesen, Ulla; Knuthsen, Pia; Leth, Torben

doi:10.1016/s0021-9673(97)01061-3

Cited by 493 publications

(326 citation statements)

References 15 publications

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“…This has been reported by other authors, who have used the same method of extraction and analysis of major Flavonoid aglycones [1, 7,8]. Generally, reversed phase HPLC with C18 columns is the most popular technique for the analysis of polyphenols in different foods, despite the fact that the separation of procyanidins is not satisfactory with these phases [9]. Spherisorb C18 stationary phase, which was used in this study to separate phenolic acids and flavonoids in aromatic plants, produce quite good results.…”

Section: Resultssupporting

confidence: 52%

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

Proestos¹,

Varzakas²

2017

Preprint

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Abstract:The antioxidant properties and polyphenol content of some selected aromatic plants grown in Greece were studied. Plants were refluxed with 60% methanol after acid hydrolysis. The phenolic substances were quantified by HPLC-DAD. The antioxidant capacity of the extracts was determined with the Rancimat test using sunflower oil as substrate. Free radical scavenging activity was measured using the stable free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Results were compared with standard BHT and ascorbic acid. Total phenol concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard.

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Section: Resultssupporting

confidence: 52%

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

Proestos¹,

Varzakas²

2017

Preprint

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“…It was reported that EA shows different pharmacological effects, including anti-inflammatory, antioxidant and chemoprevention inhibition of tumorigenesis [17][18][19][20] . Hesperidin (HES), a natural flavonoid, exists in fruits and vegetables 21 . HES also have wide pharmacological effects such as anti-inflammatory, antioxidant, anticancer and antiallergic effects [22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

A comparative study on the antioxidant effects of hesperidin and ellagic acid against skeletal muscle ischemia/reperfusion injury

Akdemir

Gülçın

Karagöz

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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The antioxidant effects of ellagic acid (EA) and hesperidin (HES) against skeletal muscle ischemia/reperfusion injury (I/R) were performed. Hindlimb ischemia has been induced by tourniquet occlusion for 2 h on left hindlimb. At the end of ischemia, the tourniquate has been removed and initiated reperfusion for 2 h. EA (100 mg/kg) has been applied orally before ischemia/reperfusion in the EA + I/R group. HES (100 mg/kg) has been given orally in the HES + I/R group. The left gastrocnemius muscle has been harvested and stored immediately at À80 C until assessed for the levels of MDA and antioxidant enzymes activities. MDA level has statistically increased in I/R group (p50.05) compared to other groups. The muscle tissue antioxidant enzymes activities were lower than the other groups in the I/R group (p50.05). EA and HES treatments significantly reversed the damage level in I/R, also activity of tissue SOD increased in the EA + I/R and HES + I/R groups.

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“…Picos correspondentes à miricetina e à quercetina não costumam ser encontrados em suas formas agliconas nos vegetais. 24 Eles podem ter sido produzidos por degradação de polifenóis glicosilados durante os processos de fracionamento das amostras, conforme sugerido por Chirinos e colaboradores. 11 As concentrações dos demais compostos identificados na Fração II (Tabela 2) foram maiores que as encontradas na literatura em extrato bruto de folhas de I. edulis, 4 à exceção da epicatequina.…”

Section: Resultsunclassified

Enriquecimento de compostos fenólicos de folhas de Inga edulis por extração em fase sólida: quantificação de seus compostos majoritários e avaliação da capacidade antioxidante

Dias¹,

Souza²,

Rogez³

2010

Quím. Nova

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Recebido em 31/10/08; aceito em 2/7/09; publicado na web em 8/12/09 PURIFICATION OF PHENOLIC COMPOUNDS FROM Inga edulis LEAVES USING SOLID-PHASE EXTRACTION: MAJOR COMPOUNDS QUANTIFICATION AND ANTIOXIDANT CAPACITY EVALUATION. A phenolic fraction was obtained from of the acetone-water-acetic acid extract of Inga edulis leaves, by liquid-liquid partition and SPE-C 18 cartridges. This method provided an increase of 108, 66, 51, 50 and 36% of flavonols, proanthocyanidins, total polyphenols, gallotannins and flavanols, respectively. The major phenolics in purified fraction were procyanidin B2, catechin and myricetin-3-O-a-L-rhamnopyranoside, which achieved increases of 111, 47 and 45%, respectively, after SPE. Acid hydrolysis confirmed the presence of procyanidins, prodelphinidins and glycosylated flavonoids.

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Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by high-performance liquid chromatography with photo-diode array and mass spectrometric detection

Cited by 493 publications

References 15 publications

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

Aromatic Plants: Antioxidant Capacity and Polyphenol Characterisation

A comparative study on the antioxidant effects of hesperidin and ellagic acid against skeletal muscle ischemia/reperfusion injury

Enriquecimento de compostos fenólicos de folhas de Inga edulis por extração em fase sólida: quantificação de seus compostos majoritários e avaliação da capacidade antioxidante

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