Quantitative analysis of DNA orientation in stationary AC electric fields using fluorescence anisotropy

Suzuki, Seiichi; Yamanashi, T.; Tazawa, S.; Kurosawa, Osamu; Washizu, Masao

doi:10.1109/28.658723

Cited by 65 publications

(68 citation statements)

References 13 publications

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“…This is partly owing to the complex local conditions that exist during application of the electric field. Orientation as a function of frequency, electric field, pH, and cation concentration has been studied using fluorescence anisotropy 23 and intensity measurements 17 to quantify the accumulation of DNA around the electrodes and the orientation of the DNA relative to the electric field lines. However, most DNA dielectrophoresis experiments reported in the literature were performed with fluorescently labeled DNA molecules free in solution which are attracted to the electrodes as a result of the imposed dielectrophoretic force.…”

Section: Introductionmentioning

confidence: 99%

Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA

Germishuizen

Tosch

Middelberg

et al. 2004

Journal of Applied Physics

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Articles you may be interested inThe authors investigate the elongation and orientation of different-sized deoxyribose nucleic acid (DNA) molecules, tethered onto gold electrodes via a terminal thiol, under the influence of high frequency ac electric fields. The DNA molecules are elongated from a random coil into an extended conformation and orientated along the electric field lines as a result of the forces acting on the molecules during the application of the ac electric fields. Elongation was observed in the frequency range 100 kHz-1 MHz, with field strengths of 0.06-1.0 MV/ m. Maximum elongation for all DNA fragments tested, irrespective of size, was found for frequencies between 200 and 300 kHz. The torque acting on the induced dipole in the DNA molecules, complemented by a directional bias force, opposite in direction to the dielectrophoretic force, provides the main contribution to the elongation process. The length of elongation is limited to either half the distance between opposing electrodes or to the contour length of the DNA, whichever is shorter. Further, the authors show that the normalized length of the elongated DNA molecules is independent of the contour length of the DNA.

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Section: Introductionmentioning

confidence: 99%

Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA

Germishuizen

Tosch

Middelberg

et al. 2004

Journal of Applied Physics

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show abstract

“…In contrast to many dielectrophoresis experiments in which the DNA is free in solution [4][5][6][9][10][11][12][13], here the DNA is tethered to a surface by strong thiol-gold bonds. The average maximum length of the elongated DNA depends on the frequency and the magnitude of the ac field [4].…”

Section: Dielectrophoretic Stretching Of Surface-bound Dnamentioning

confidence: 99%

“…DNA is a polyelectrolyte, and in solution the phosphate groups on the sugar-phosphate backbone dissociate to form a negatively charged molecule surrounded radially by a cloud of positively charged counterions [4][5][6]. This makes the DNA highly polarizable, and in an electric field a dipole is induced in the molecule.…”

Section: Introductionmentioning

confidence: 99%

“…In a non-uniform electric field, DNA molecules will experience a dielectrophoretic force and an orientational torque as a result of the interaction between the induced DNA dipole and the electric field [5,7,8]. The torque and the dielectrophoretic force are a function of the magnitude and frequency of the electric field, the dielectric properties of the medium and the DNA, and the size of the DNA [5,7]. Dielectrophoresis is a non-contact manipulation technique enabling positioning and orientation of DNA molecules in nanotechnological devices with a high spatial resolution, and is becoming increasingly popular.…”

Section: Introductionmentioning

confidence: 99%

“…This makes the DNA highly polarizable, and in an electric field a dipole is induced in the molecule. In a non-uniform electric field, DNA molecules will experience a dielectrophoretic force and an orientational torque as a result of the interaction between the induced DNA dipole and the electric field [5,7,8]. The torque and the dielectrophoretic force are a function of the magnitude and frequency of the electric field, the dielectric properties of the medium and the DNA, and the size of the DNA [5,7].…”

Section: Introductionmentioning

confidence: 99%

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Selective dielectrophoretic manipulation of surface-immobilized DNA molecules

et al. 2003

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The fabrication of nanoscale molecular devices is becoming increasingly important and research into their fabrication has intensified over the last few years. In particular, the attachment of molecular objects onto various surfaces has attracted considerable attention. Here, we report a multistep surface immobilization procedure, which allows the specific and controlled attachment of very long DNA molecules onto gold electrodes. Further, we report the effect of dielectrophoresis on these surface-bound DNA molecules with respect to amplitude and frequency, and we show that selected surface-immobilized DNA molecules can be manipulated by dielectrophoresis. Finally, we investigated the use of dielectrophoresis in conjunction with the multistep surface immobilization of fluorescently labelled, surface-bound λ-DNA in a basic data-storage device.

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Orientation and Positioning of DNA Molecules with an Electric Field Technique

2002

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A controlled handling of single molecules is essential for the fabrication and the investigation of devices based on molecules. We present here the implementation of an electric field based method used to manipulate DNA molecules by means of lithographically patterned metallic electrodes. We optimized the geometry of the lithographic structures to favor a precise positioning of the molecules via dielectrophoresis. This process is combined with an orientation of the molecules parallel to the electric field lines due to their induced dipole moment. The relatively high polarizability of the DNA molecules in solution is essential to achieve these manipulations. We expect this method to be softer than the stretching of molecules using a receding meniscus. The visualization of the molecules was achieved using fluorescence microscopy.

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Quantitative analysis of DNA orientation in stationary AC electric fields using fluorescence anisotropy

Cited by 65 publications

References 13 publications

Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA

Influence of alternating current electrokinetic forces and torque on the elongation of immobilized DNA

Selective dielectrophoretic manipulation of surface-immobilized DNA molecules

Orientation and Positioning of DNA Molecules with an Electric Field Technique

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