Core-shell magnetic nanoparticles have received significant attention recently and are actively investigated owing to their large potential for a variety of applications. Here, the synthesis and characterization of bimetallic nanoparticles containing a magnetic core and a gold shell are discussed. The gold shell facilitates, for example, the conjugation of thiolated biological molecules to the surface of the nanoparticles. The composite nanoparticles were produced by the reduction of a gold salt on the surface of pre-formed cobalt or magnetite nanoparticles. The synthesized nanoparticles were characterized using ultraviolet-visible absorption spectroscopy, transmission electron microscopy, energy dispersion X-ray spectroscopy, X-ray diffraction and super-conducting quantum interference device magnetometry. The spectrographic data revealed the simultaneous presence of cobalt and gold in 5.6±0.8 nm alloy nanoparticles, and demonstrated the presence of distinct magnetite and gold phases in 9.2±1.3 nm core-shell magnetic nanoparticles. The cobalt-gold nanoparticles were of similar size to the cobalt seed, while the magnetite-gold nanoparticles were significantly larger than the magnetic seeds, indicating that different processes are responsible for the addition of the gold shell. The effect on the magnetic properties by adding a layer of gold to the cobalt and magnetite nanoparticles was studied. The functionalization of the magnetic nanoparticles is demonstrated through the conjugation of thiolated DNA to the gold shell.
The ability to detect low concentrations of biomarkers in patient samples is one of the cornerstones of modern healthcare. In general, biosensing approaches are based on measuring signals resulting from the interaction of a large ensemble of molecules with the sensor. Here, we report a biosensor platform using DNA origami featuring a central cavity with a target-specific DNA aptamer coupled with a nanopore read-out to enable individual biomarker detection. We show that the modulation of the ion current through the nanopore upon the DNA origami translocation strongly depends on the presence of the biomarker in the cavity. We exploit this to generate a biosensing platform with a limit of detection of 3 nM and capable of the detection of human C-reactive protein (CRP) in clinically relevant fluids. Future development of this approach may enable multiplexed biomarker detection by using ribbons of DNA origami with integrated barcoding.
We report on the use of standing surface acoustic waves, formed on a single-crystal piezoelectric substrate, to organize micron-scale latex particles into an array comprising a series of lines in an adjacent microfluidic system. The lines of particles are formed parallel to the substrate surface and perpendicular to the surface acoustic wave vector. They extend across the width of the acoustic beam aperture, with a periodicity of one-half the surface acoustic wavelength. The position and spacing of the particle arrays can be altered by adjusting the acoustic wave frequency within the device passband. We discuss the mechanism responsible for the formation of the lines, which could be widely applicable to the alignment of microscopic objects held in suspension.
The two-dimensional concentration and manipulation of micron-scale particles by orthogonal, surface acoustic, standing waves is demonstrated. The particles are organized by liquid pressure waves in a microfluidic system over a piezoelectric substrate and form a uniform two-dimensional array with a spacing governed by the mechanical nodes of the two orthogonal, surface acoustic, standing waves. The nodal spacing can be controlled in each orthogonal direction independently by adjustment of the radio frequency applied to the separate acoustic wave transducers. This technique could be used to enhance the particle concentrations at sensing locations in DNA or protein array detectors.
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