Quantitative analysis of different respiratory specimens on two automated test systems for detection of SARS-CoV-2 RNA

Lübke, Nadine; Repges, Katharina; Menne, Christopher; Walker, Andreas; Jensen, Björn‐Erik Ole; Freise, Noemi F.; Gliga, Smaranda; Eickhoff, Simon B.; Bosse, Hans Martin; Adams, Ortwin; Timm, Jörg

doi:10.1016/j.diagmicrobio.2022.115800

Cited by 1 publication

(2 citation statements)

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“…Many saliva tests utilize a quantitative reverse transcription-polymerase chain reaction (RT-qPCR) to detect viral RNA in subject saliva samples [133]. The demand for SARS-CoV-2 diagnostic testing catalyzed the development of different sampling technologies that test for viral RNA using RT-qPCR, including nasal and pharyngeal swabs [134]. RT-qPCR testing is particularly beneficial due to its relatively low cost and high sensitivity [135].…”

Section: Accurate Alternatives To Saliva-based Diagnosticsmentioning

confidence: 99%

“…RT-qPCR testing is particularly beneficial due to its relatively low cost and high sensitivity [135]. While some have reported as much as 20% higher sensitivity with diagnostic assays utilizing nasopharyngeal swabs when compared to saliva samples, saliva samples provide broader testing accessibility and allow for more individuals to be tested in a more comfortable and minimally invasive manner [134]. Of note, self-administered nasopharyngeal and oral swabs, including those used in at-home testing kits, have been utilized in multiplex RT-qPCR analysis assays with an accuracy comparable to tests administered by healthcare professionals [40,41].…”

Section: Accurate Alternatives To Saliva-based Diagnosticsmentioning

confidence: 99%

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Herpesviruses and SARS-CoV-2: Viral Association with Oral Inflammatory Diseases

Banks,

Capistrano,

Brandini

et al. 2024

Pathogens

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The oral cavity is a niche for diverse microbes, including viruses. Members of the Herpesviridae family, comprised of dsDNA viruses, as well as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an ssRNA virus, are among the most prevalent viruses infecting the oral cavity, and they exhibit clinical manifestations unique to oral tissues. Viral infection of oral mucosal epithelia triggers an immune response that results in prolonged inflammation. The clinical and systemic disease manifestations of HHV have been researched extensively, and several recent studies have illuminated the relationship between HHV and oral inflammatory diseases. Burgeoning evidence suggests the oral manifestation of SARS-CoV-2 infection includes xerostomia, dysgeusia, periodontal disease, mucositis, and opportunistic viral and bacterial infections, collectively described as oral post-acute sequelae of COVID-19 (PASC). These diverse sequelae could be a result of intensified immune responses initially due to the copious production of proinflammatory cytokines: the so-called “cytokine storm syndrome”, facilitating widespread oral and non-oral tissue damage. This review explores the interplay between HHV, SARS-CoV-2, and oral inflammatory diseases such as periodontitis, endodontic disease, and peri-implantitis. Additionally, the review discusses proper diagnostic techniques for identifying viral infection and how viral diagnostics can lead to improved overall patient health.

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Section: Accurate Alternatives To Saliva-based Diagnosticsmentioning

confidence: 99%