Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS

Gao, Xin; Liu, Wenru; Mei, Jun; Xie, Jing

doi:10.3390/molecules24244609

Cited by 16 publications

(14 citation statements)

References 80 publications

(76 reference statements)

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“…5 c–f), thereby conferring tolerance to abiotic stress by facilitating the membrane fluidity [ 21 ]. These data implied the GPAT2 overexpression facilitated the lipid remodeling via the reduction of glycolipid, which could be used for the phospholipid generation, which is in accordance with the previous report [ 22 ]. While at the same time, glycolipids content was found to be decreased in the transgenic cells and no such reduction in glycolipid was observed in WT (Additional file 1 : Fig.…”

Section: Resultssupporting

confidence: 92%

TAG pathway engineering via GPAT2 concurrently potentiates abiotic stress tolerance and oleaginicity in Phaeodactylum tricornutum

Wang¹,

Liu²,

Li³

et al. 2020

Biotechnol Biofuels

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Background Despite the great potential of marine diatoms in biofuel sector, commercially viable biofuel production from native diatom strain is impractical. Targeted engineering of TAG pathway represents a promising approach; however, recruitment of potential candidate has been regarded as critical. Here, we identified a glycerol-3-phosphate acyltransferase 2 (GPAT2) isoform and overexpressed in Phaeodactylum tricornutum. Results GPAT2 overexpression did not impair growth and photosynthesis. GPAT2 overexpression reduced carbohydrates and protein content, however, lipid content were significantly increased. Specifically, TAG content was notably increased by 2.9-fold than phospho- and glyco-lipids. GPAT2 overexpression elicited the push-and-pull strategy by increasing the abundance of substrates for the subsequent metabolic enzymes, thereby increased the expression of LPAAT and DGAT. Besides, GPAT2-mediated lipid overproduction coordinated the expression of NADPH biosynthetic genes. GPAT2 altered the fatty acid profile in TAGs with C16:0 as the predominant fatty acid moieties. We further investigated the impact of GPAT2 on conferring abiotic stress, which exhibited enhanced tolerance to hyposaline (70%) and chilling (10 ºC) conditions via altered fatty acid saturation level. Conclusions Collectively, our results exemplified the critical role of GPAT2 in hyperaccumulating TAGs with altered fatty acid profile, which in turn uphold resistance to abiotic stress conditions.

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Section: Resultssupporting

confidence: 92%

TAG pathway engineering via GPAT2 concurrently potentiates abiotic stress tolerance and oleaginicity in Phaeodactylum tricornutum

Wang¹,

Liu²,

Li³

et al. 2020

Biotechnol Biofuels

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“…More recently, the study of a slow-growing marine bacterium, Vibrio splendidus, in presence of exogenous FAs has also shown a change in the acyl chain profile as a function of the growth phase [8]. This observation is not surprising since bacteria are known to adapt very quickly to their environment, and for example to change their membrane properties by adjusting their lipid composition, i.e., both their FAs and headgroup profiles, depending on the growth conditions [11][12][13][14][15][16][17][18]. In humans as well, alteration 1 B. subtilis: Bacillus subtilis 168; BCFAs: branched chain fatty acids; CDCl 3 : deuterochloroform; CL: cardiolipin; DCM-MeOH: dichloromethane-methanol; DPC: dodecylphosphocholine; E. coli: Escherichia coli BL21; EDTA: ethylenediaminetetraacetic free acid; Egg SM: chicken egg sphingomyelin (mostly N-palmitoyl-D-erythro-sphingosylphosphorylcholine); FAs: fatty acids; FAME mix: fatty acid methyl ester mix C4-C24; GCMS: gas chromatography combined with mass spectrometry; LCMS: liquid chromatography combined with mass spectrometry; LPG: lysyl-phosphatidylglycerol; M 2 : second spectral moment of 2 H SS-NMR spectra; MAS: magic-angle spinning; OA: oleic acid or octadecenoic acid or C18:1; OD 600 : optical density at 600 nm; PA: palmitic acid or hexadecanoic acid or C16:0; PA-d 31 : perdeuterated palmitic acid; PE: phosphatidylethanolamine; PG: phosphatidylglycerol; PhA: phosphatidic acid; POPA: 1palmitoyl-2-oleoyl-sn-glycero-3-phosphate; POPC: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine; POPE : 1-palmitoyl-2-oleoyl-snglycero-3-phosphoethanolamine; POPG : 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol); POPS: 1-palmitoyl-2-oleoyl-snglycero-3-phospho-L-serine; PS: phosphatidylserine; SS-NMR: solid-state Nuclear Magnetic Resonance; TMCL: 1',3'-bis[1,2dimyristoyl-sn-glycero-3-phospho]-glycerol; TMP: trimethyl phosphate; Tween® 20: polyoxyethylene sorbitan monolaurate; UFAs: unsaturated fatty acids; SFAs: non-branched saturated fatty acids; S/U: saturated/unsaturated.…”

Section: Introductionmentioning

confidence: 99%

Growth-phase dependence of bacterial membrane lipid profile and labeling for in-cell solid-state NMR applications

Laydevant

Mahabadi

Llido

et al. 2022

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Cell labeling is a preliminary step in multiple biophysical approaches, including the solid-state nuclear magnetic resonance (NMR) study of bacteria in vivo. Deuterium solid-state NMR has been used in the past years to probe bacterial membranes and their interactions with antimicrobial peptides, following a standard labeling protocol. Recent results from our laboratory on a slow-growing bacterium has shown the need to optimize this protocol, especially the bacterial growth time before harvest and the concentration of exogenous labeled fatty acids to be used for both Escherichia coli and Bacillus subtilis. It is also essential for the protocol to remain harmless to cells while providing optimal labeling.We have therefore developed a fast and facile approach to monitor the lipid composition of bacterial membranes under various growth conditions, combining solution 31 P NMR and GCMS. Using this approach, the optimized labeling conditions of Escherichia coli and Bacillus subtilis with deuterated palmitic acid were determined. Our results show a modification of B. subtilis phospholipid profile as a function of the growth stage, as opposed to E. coli. Our protocol recommends low concentrations of exogenous palmitic acid in the growth medium, and bacteria harvest after the exponential phase.

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“…Ectotherms, such as fish, acclimatize to varying temperatures by modulation of the proportion of unsaturated fatty acids in membranes to maintain membrane order (Ernst et al, 2016). This adaptive response, known as homeoviscous adaptation (HVA; Hazel & Williams, 1990; Sinensky, 1974), has been widely observed in ectotherm, such as fishes (Tiku et al, 1996), phototrophic sponges (Bennett et al, 2018), micro‐organisms (Gao et al, 2019; Řezanka et al, 2016), and bivalves (Pernet et al, 2007). However, due to the tremendous number of structurally and functionally diverse lipids in organisms, roles of lipids in response to thermal stimuli have not yet been fully described or understood.…”

Section: Introductionmentioning

confidence: 99%

Remodeling of Arctic char (Salvelinus alpinus) lipidome under a stimulated scenario of Arctic warming

Wang

Gong

Deng

et al. 2021

Global Change Biology

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Arctic warming associated with global climate change poses a significant threat to populations of wildlife in the Arctic. Since lipids play a vital role in adaptation of organisms to variations in temperature, high‐resolution mass‐spectrometry‐based lipidomics can provide insights into adaptive responses of organisms to a warmer environment in the Arctic and help to illustrate potential novel roles of lipids in the process of thermal adaption. In this study, we studied an ecologically and economically important species—Arctic char (Salvelinus alpinus)—with a detailed multi‐tissue analysis of the lipidome in response to chronic shifts in temperature using a validated lipidomics workflow. In addition, dynamic alterations in the hepatic lipidome during the time course of shifts in temperature were also characterized. Our results showed that early life stages of Arctic char were more susceptible to variations in temperature. One‐year‐old Arctic char responded to chronic increases in temperature with coordinated regulation of lipids, including headgroup‐specific remodeling of acyl chains in glycerophospholipids (GP) and extensive alterations in composition of lipids in membranes, such as less lyso‐GPs, and more ether‐GPs and sphingomyelin. Glycerolipids (e.g., triacylglycerol, TG) also participated in adaptive responses of the lipidome of Arctic char. Eight‐week‐old Arctic char exhibited rapid adaptive alterations of the hepatic lipidome to stepwise decreases in temperature while showing blunted responses to gradual increases in temperature, implying an inability to adapt rapidly to warmer environments. Three common phosphatidylethanolamines (PEs) (PE 36:6|PE 16:1_20:5, PE 38:7|PE 16:1_22:6, and PE 40:7|PE 18:1_22:6) were finally identified as candidate lipid biomarkers for temperature shifts via machine learning approach. Overall, this work provides additional information to a better understanding of underlying regulatory mechanisms of the lipidome of Arctic organisms in the face of near‐future warming.

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Quantitative Analysis of Cold Stress Inducing Lipidomic Changes in Shewanella putrefaciens Using UHPLC-ESI-MS/MS

Cited by 16 publications

References 80 publications

TAG pathway engineering via GPAT2 concurrently potentiates abiotic stress tolerance and oleaginicity in Phaeodactylum tricornutum

TAG pathway engineering via GPAT2 concurrently potentiates abiotic stress tolerance and oleaginicity in Phaeodactylum tricornutum

Growth-phase dependence of bacterial membrane lipid profile and labeling for in-cell solid-state NMR applications

Remodeling of Arctic char (Salvelinus alpinus) lipidome under a stimulated scenario of Arctic warming

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