Quantitative analysis of Caenorhabditis elegans chemotaxis using a microfluidic device

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

“…Currently, several dilution micro-devices have been proposed for generating logarithmic, liner, and parabolic chemical concentration gradients [28][29][30][31][32][33][34][35][36]. Compared to traditional methods, microfluidic devices could generate linear chemical concentration gradient which shown great potential in studying chemotaxis behavior [22,[37][38][39], screening of chemotaxis-defective mutants [23,40] and drug screening in C. elegans [20,37,41]. Although these "Christmas tree" mixers had been well established and applied to create concentration gradients, they are not suitable for generating wide-range concentration gradient, such as 1000 fold dilution.…”

“…In recent years, microfluidic device has been recognized as a new platform for fundamental studies in C. elegans because of microchannel's comparable dimension to the tiny worms and the versatile use of micro-flow. "Worm-chip" have successfully used in phenotyping and stage screening, nerve system imaging, behavioral dynamics, microsurgery and microinjection [18][19][20][21][22][23][24][25][26][27]. Currently, several dilution micro-devices have been proposed for generating logarithmic, liner, and parabolic chemical concentration gradients [28][29][30][31][32][33][34][35][36].…”

Single-cell Analysis with Microfluidic Devices

“…Most recently, a microfluidic device has emerged as a powerful instrument for neurology and behavior investigations of small model organisms, such as C. elegans, zebrafish and Drosophila. In view of this, our group have reported a series of studies about the C. elegans on microfludic chips, [77][78][79][80] one of these studies is shown in Furthermore, the mechanism of intercellular calcium signaling was investigated by analyzing the ICW propagation. Since C. elegans is a well-established model animal for studying the biological processes in organisms, Bosari et al 81 performed a microfluidic platform capable of acquiring high-throughput imaging of subcellular features in C. elegans with lifelong high-resolution.…”

Microfluidic Approaches for Manipulating, Imaging, and Screening C. elegans

“…While the above setup enables single animal-based studies, chemotaxis assays are performed typically in batches consisting of hundreds of animals. A microfluidic device, developed by Hu et al [ 60 ], simplifies such population-based experiments ( Figure 6 B). Repetitive flow splitting and mixing microchannels arranged in a tree-like design were used to generate linear gradients of sodium chloride (NaCl) across the device.…”