Quantitative analysis of adhesion of cariogenic streptococci to orthodontic raw materials

Abstract

Introduction: Knowledge of adhesion patterns of cariogenic streptococci to orthodontic materials can provide valuable information on the cause of enamel demineralization during orthodontic treatment. The purpose of this study was to investigate the adhesion of 2 cariogenic streptococci strains to 7 orthodontic raw materials (3 light-cured orthodontic adhesives, 3 bracket raw materials, and hydroxyapatite) with respect to bacterial species, incubation time, and saliva coating. Methods: Each material was incubated with unstimulated whole saliva or phosphate-buffered saline solution for 2 hours. Binding assays were then performed by incubating tritium-labeled cariogenic streptococci with each raw material for 3 or 6 hours. Results: The degree of adhesion varied by material type. Generally, adhesion of cariogenic streptococci was significantly higher for bonding adhesives than for bracket materials, and adhesion to resin-modified glass ionomer was the highest. A longer incubation time generally increased bacterial adhesion, whereas saliva coating did not significantly influence bacterial adhesion. Conclusions: Bonding adhesives around brackets should be removed carefully during the bonding procedure to avoid enamel decalcification. (Am J Orthod Dentofacial Orthop 2008;133:882-8)

Effect of orthodontic bonding steps on the initial adhesion of mutans streptococci in the presence of saliva

“…6 The disks were divided into four groups according to surface treatment type: no surface treatment control (HA), acid-etched (HE), primed using a conventional primer after acidetching (HP), and primed using a self-etching primer (SEP).…”

“…Transbond XT composite adhesive (3M/Unitek) disks were prepared to the same uniform size using Teflon templates in the adhesive (AD) group as previously described. 6 Unstimulated whole saliva (UWS) was collected from healthy volunteers as previously described. 7 Each saliva sample was centrifuged at 3500g for 10 minutes to remove any cellular debris, and the resulting supernatant was used after filter sterilization through a Stericup & Streitop (Millipore, Billerica, Mass).…”

Use of the checkerboard DNA–DNA hybridisation technique for in vivo detection of cariogenic microorganisms on metallic brackets, with or without use of an antimicrobial agent

“…Amongst these techniques, the checkerboard DNA-DNA hybridisation (CDDH) is a method largely used in dentistry, including Periodontics, 10 Endodontics, 11 Implantology, 12 Paediatric dentistry, 13 Cariology, 14 and Orthodontics. 1 Although the oral hygiene methods in orthodontic patients should be emphasised, 2,8 the manual skills of children, youngsters and elderly can be compromised sometimes and affect the mechanical dental biofilm control in these individuals. According to Smiech-Slomkowska and Jablonska-Zrobek, 15 the regular toothbrushing and use of dental floss alone do not reduce the salivary levels of S. mutans and Lactobacillus in patients wearing orthodontic appliances.…”

Effect of Bisphenol A Glycol Methacrylate on Virulent Properties of <b><i>Streptococcus mutans</i></b> UA159

“…Unstimulated whole saliva was collected by the spitting method from healthy volunteers as previously described [Lim et al, 2008]. Saliva samples were centrifuged at 3,500 g for 10 min to remove any cellular debris, and the resulting supernatant was used after filter sterilization through a Millex-GP (0.22-μm filter; Millipore, Darmstadt, Germany).…”