Use of the checkerboard DNA–DNA hybridisation technique for in vivo detection of cariogenic microorganisms on metallic brackets, with or without use of an antimicrobial agent

Filho, Paulo Nelson; Olmedo, Lourdes Yanissely Garcia; Andrucioli, Marcela Cristina Damião; Saraiva, Maria da Conceição Pereira; Matsumoto, Mírian Aiko Nakane; Queiroz, Alexandra Mussolino de; Silva, Léa Assed Bezerra da

doi:10.1016/j.jdent.2011.05.001

Cited by 25 publications

(22 citation statements)

References 21 publications

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“…Several studies have attempted to elucidate the bacterial colonization of brackets and its impact on the oral microbiota and clinical outcomes of individuals under orthodontic treatment [23,24]. The amount of microbial species colonizing the formed biofilm, its complexity, and host factors have a relevant impact on the maintenance of oral health and in the development of periodontal disease and bone and dentin reabsorption [25,26].…”

Section: Discussionmentioning

confidence: 99%

Microbial complexes levels in conventional and self-ligating brackets

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Microbial complexes levels in conventional and self-ligating brackets

et al. 2016

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“…Dental biofilm formation is a result of an increase in the number of microorganism that is directly related to dental caries, particularly Streptococcus mutans (S. mutans) (2)(3)(4)(5)(6)(7). Dental biofilm formation on tooth surfaces is 2-3 times higher in orthodontic patients than high plaque-forming adults with no orthodontic treatment (1).…”

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confidence: 99%

Effects of chlorhexidine (gel) application on bacterial levels and orthodontic brackets during orthodontic treatment

et al. 2016

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The objectives of this study were to evaluate the effects of applying 0.50% chlorhexidine (CHX) gel using the dental drug delivery system (3DS) on salivary Streptococcus mutans (S. mutans) and on the surface topography of metal and ceramic orthodontic brackets. The study involved 20 orthodontic patients with high levels of salivary S. mutans. The patients were treated with professional mechanical tooth cleaning followed by application of 0.50% CHX using individual trays (3DS). Salivary S. mutans levels were repeatedly measured 1, 2, 4, and 8 weeks post-treatment. In vitro study utilized forty ceramic and metallic brackets that were immersed in 0.50% CHX gel for 10 min, whereas another untreated forty brackets served as controls. The frictional resistances of stainless steel wires to the brackets before and after CHX treatment were recorded using a universal testing machine. Scanning electron microscopy was used to compare changes in the surface topography of brackets. Statistical analyses were used to determine the effect of CHX on bacterial count and to evaluate the effect of CHX on frictional resistance. According to the results of this study, S. mutans levels were reduced significantly (P < 0.05). There were no significant changes in the frictional resistance and surface topography of brackets before or after application of CHX. (J Oral Sci 58, [35][36][37][38][39][40][41][42] 2016)

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“…There has been great concern in evaluating the impact of orthodontic appliance use on the salivary levels of microorganisms or biofilm accumulation on tooth surfaces (Derks et al, ; Leung et al, ; Rosenbloom & Tinanoff, ; Sudjalim, Woods, Manton, & Reynolds, ) which favors the development of caries lesions (Ahn et al, ; Steinberg & Eyal, ). However, few authors have investigated in situ the presence of bacterial biofilms on the surface of orthodontic appliances' components, being limited to studies on brackets (Andrucioli et al, , Bergamo et al, ; Brêtas et al, , Nelson‐Filho et al, ,b), acrylic baseplates of removable appliances (Lessa et al, ; Peixoto et al, ) and elastomeric ligatures (Magno et al, ) alone. To the best of our knowledge, one in situ study has evaluated the contamination by MS and the efficacy of a clinical disinfection protocol to reduce the formation of MS colonies/biofilms (Maruo et al, ), but no in various components (acrylic baseplate, screw, bands, and wires) of Haas expanders.…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation in Haas palatal expanders with and without use of an antimicrobial agent: an in situ study

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Andrucioli

Ferreira

et al. 2016

Microscopy Res & Technique

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Orthodontic appliances causes specific alterations in oral environment, including reduction of pH, increase of dental biofilm and elevation of salivary microbial levels, causing an increased risk for dental caries. This study evaluated, using microbial culture and scanning electron microscopy (SEM), the in situ contamination by mutans streptococci (MS) of different surfaces of Haas palatal expanders with and without use of chlorhexidine gluconate mouthrinses (CHX). Thirty-four patients were randomly assigned to two groups (n = 17/group), using placebo (Group I) and 0.12% CHX (Group II-Periogard ) mouthrinses twice a week. After 4 months, appliances were submitted to microbiological processing and after fragments were analyzed by SEM. Mann-Whitney U test (α = 5%) was used to assess differences between groups on the appliances' different surfaces and to compare the contamination on the free and nonfree surfaces of these components. There was no difference (p = 0.999) between groups regarding the number of MS colonies/biofilms on the nonfree surfaces, which showed intense contamination. However, free surfaces of Group II presented less contamination (p < 0.001) than those of Group I in all appliances' components. Results of the microbial culture were confirmed by SEM. Use of 0.12% CHX was effective in reducing the formation of MS colonies/biofilms on free surfaces of Haas expanders, in situ.

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Use of the checkerboard DNA–DNA hybridisation technique for in vivo detection of cariogenic microorganisms on metallic brackets, with or without use of an antimicrobial agent

Cited by 25 publications

References 21 publications

Microbial complexes levels in conventional and self-ligating brackets

Microbial complexes levels in conventional and self-ligating brackets

Effects of chlorhexidine (gel) application on bacterial levels and orthodontic brackets during orthodontic treatment

Biofilm formation in Haas palatal expanders with and without use of an antimicrobial agent: an in situ study

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