Quantitative Analysis, InfraredUpdate based on the original article by Frederic Cadet,<i>Encyclopedia of Analytical Chemistry</i>, © 2000, John Wiley &amp; Sons, Ltd.

Cadet, Frédéric; Garrigues, Salvador; Guárdia, Miguel de la

doi:10.1002/9780470027318.a5610.pub2

Cited by 7 publications

(5 citation statements)

References 212 publications

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“…The role of the nπ* state in nucleobase relaxation is crucial to understanding DNA damage as this state has been implicated in the formation of unwanted photoproducts and as an intermediate in the formation of the 3 ππ* state . For example, one of the most prominent pathways of DNA damage, thymine dimerization, occurs when covalent bonds are formed between adjacent thymine nucleobases in DNA strands, most commonly as cyclobutane pyrimidine dimers or 6–4 photoproducts which are both likely formed through a long-lived intermediate state. , Because these long-lived excited states are not observed here in the relaxation of single subunits of T, Thd, or TMP, this excited state trapping process may likely be the result of or enhanced by interaction between bases.…”

Section: Discussionmentioning

confidence: 99%

Relaxation Dynamics of Hydrated Thymine, Thymidine, and Thymidine Monophosphate Probed by Liquid Jet Time-Resolved Photoelectron Spectroscopy

et al. 2019

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Probed by Liquid Jet Time-Resolved Photoelectron Spectroscopy. ChemRxiv. Preprint. The relaxation dynamics of thymine and its derivatives thymidine and thymidine monophosphate were studied using time-resolved photoelectron spectroscopy applied to a water microjet. Two absorption bands were studied, the first is a bright ππ* state which was populated using tunable-ultraviolet light in the range of 4.74-5.17 eV and probed using a 6.20 eV probe pulse. By reversing the order of these pulses, a band containing multiple ππ* states was populated by the 6.20 eV pulse and the lower energy pulse served as the probe. The lower lying ππ* state was found to decay in ~400 fs in both thymine and thymidine independent of pump photon energy while thymidine monophosphate decays varied from 670-840 fs with some pump energy dependence. The application of a computational QM/MM scheme at the XMS-CASPT2//CASSCF/AMBER level of theory suggests that conformational differences existing between thymidine and thymidine monophosphate in solution accounts for this difference. The higher lying ππ* band was found to decay in ~600 fs in all three cases, but was only able to be characterized when using the 5.17 eV probe pulse. Notably, no long-lived signal from an np* state could be identified in either experiment on any of the three molecules. File list (3) download file view on ChemRxiv Thymine_to_submit.docx (1.52 MiB) download file view on ChemRxiv SI_to_submit.docx (894.21 KiB) download file view on ChemRxiv Thymine_to_submit.pdf (1.40 MiB)

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Section: Discussionmentioning

confidence: 99%

Relaxation Dynamics of Hydrated Thymine, Thymidine, and Thymidine Monophosphate Probed by Liquid Jet Time-Resolved Photoelectron Spectroscopy

et al. 2019

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“…As referenced in practically all compendium pharmacopoeias, the major use of FTIR in the transmission mode is for the identification of API in raw materials and finished products. Regardless of the many advantages described in the last two decades 28 , FTIR equipment in the pharmaceutical field for qualitative issues is widely available but still undervalued for quantitative purposes. We believe that FTIR instruments in conjunction with DS are ideal tools for pharmaceutical quality control.…”

Section: Discussionmentioning

confidence: 99%

“…For the past two decades, the transmission FTIR spectroscopy has undergone significant application for quantitative purposes, several in the pharmaceutical analysis field 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 . As suggested above, a FTIR spectrum can be used for verifying the identity and purity of API and for detecting any spectral interference originating from the excipients.…”

Section: Introductionmentioning

confidence: 99%

Transmission FTIR derivative spectroscopy for estimation of furosemide in raw material and tablet dosage form

Gallignani

Rondon

Ovalles

et al. 2014

Acta Pharmaceutica Sinica B

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A Fourier transform infrared derivative spectroscopy (FTIR-DS) method has been developed for determining furosemide (FUR) in pharmaceutical solid dosage form. The method involves the extraction of FUR from tablets with N,N-dimethylformamide by sonication and direct measurement in liquid phase mode using a reduced path length cell. In general, the spectra were measured in transmission mode and the equipment was configured to collect a spectrum at 4 cm−1 resolution and a 13 s collection time (10 scans co-added). The spectra were collected between 1400 cm−1 and 450 cm−1. Derivative spectroscopy was used for data processing and quantitative measurement using the peak area of the second order spectrum of the major spectral band found at 1165 cm−1 (SO2 stretching of FUR) with baseline correction. The method fulfilled most validation requirements in the 2 mg/mL and 20 mg/mL range, with a 0.9998 coefficient of determination obtained by simple calibration model, and a general coefficient of variation <2%. The mean recovery for the proposed assay method resulted within the (100±3)% over the 80%–120% range of the target concentration. The results agree with a pharmacopoeial method and, therefore, could be considered interchangeable.

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“…During the last few decades, there has been an exponential growth in the quantitative applications of IR spectroscopy 15 .…”

Section: Introductionmentioning

confidence: 99%

First derivative ATR-FTIR spectroscopic method as a green tool for the quantitative determination of diclofenac sodium tablets

et al. 2020

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Background: Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy is a rapid quantitative method which has been applied for pharmaceutical analysis. This work describes the utility of first derivative ATR-FTIR spectroscopy in the quantitative determination of diclofenac sodium tablets. Methods: This analytical quantitative technique depends on a first derivative measurement of the area of infrared bands corresponding to the CO stretching range of 1550-1605 cm-1. The specificity, linearity, detection limits, precision and accuracy of the calibration curve, the infrared analysis and data manipulation were determined in order to validate the method. The statistical results were compared with other methods for the quantification of diclofenac sodium. Results: The excipients in the commercial tablet preparation did not interfere with the assay. Excellent linearity was found for the drug concentrations in the range 0.2 – 1.5 w/w %. (r2= 0.9994). Precision of the method was assessed by the repeated analysis of diclofenac sodium tablets; the results obtained showed small standard deviation and relative standard deviation values, which indicates that the method is quite precise. The high percentage of recovery of diclofenac sodium tablets (99.81, 101.54 and 99.41%) demonstrate the compliance of the obtained recoveries with the pharmacopeial percent recovery. The small limit of detection and limit of quantification values (0.0528 and 0.1599 w/w %, respectively) obtained by this method indicate the high sensitivity of the method. Conclusions: First derivative ATR-FTIR spectroscopy showed high accuracy and precision, is considered as nondestructive, green, low cost and rapid, and can be applied easily for the pharmaceutical quantitative determination of diclofenac sodium tablet formulations.

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Quantitative Analysis, InfraredUpdate based on the original article by Frederic Cadet,Encyclopedia of Analytical Chemistry, © 2000, John Wiley & Sons, Ltd.

Cited by 7 publications

References 212 publications

Relaxation Dynamics of Hydrated Thymine, Thymidine, and Thymidine Monophosphate Probed by Liquid Jet Time-Resolved Photoelectron Spectroscopy

Relaxation Dynamics of Hydrated Thymine, Thymidine, and Thymidine Monophosphate Probed by Liquid Jet Time-Resolved Photoelectron Spectroscopy

Transmission FTIR derivative spectroscopy for estimation of furosemide in raw material and tablet dosage form

First derivative ATR-FTIR spectroscopic method as a green tool for the quantitative determination of diclofenac sodium tablets

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