Quantitative analysis combined with chromatographic fingerprint and antioxidant activities for the comprehensive evaluation of Compound Danshen Tablets

Chen, Jiao; Gao, Jiayue; Sun, Guoxiang

doi:10.1002/jssc.201600813

Cited by 21 publications

(10 citation statements)

References 14 publications

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“…Oxidation is an important process for the energy productive of many biological organs [50]. Studies have shown that cancer, inflammation, blood diseases, and other diseases are closely related to oxidative free radicals, and excessive free radicals in the human body will lead to aging [51][52][53].…”

Section: Antioxidant Activities Of 21 Batches Of Saffronmentioning

confidence: 99%

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

You

Zhong

et al. 2021

Journal of Chemistry

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Crocus sativus L. is commonly used as functional food and medicinal herb in traditional Chinese medicine. In this study, the spectrum–effect relationship was established between HPLC fingerprints and in vitro antioxidant activity of saffron to improve the quality evaluation method of saffron. The fingerprints of 21 batches of saffron collected from different regions were assessed, and the data were further analyzed by chemometric methods, including similarity analysis, hierarchical clustering analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The spectrum–effect relationship between fingerprints and antioxidant effect of saffron was analyzed by grey relational analysis and partial least square methods to figure out the antioxidant component of saffron. Thirteen common peaks of 21 batches of saffron were included in the analysis, and peak 3 (picrocrocin), peak 7 (crocin I), and peak 10 (crocin II) were identified as the main active components responsible for antioxidant efficacy. Besides, a multi-index quality control method was developed for simultaneous determination of these three antioxidant components in saffron. Taken together, this study provided new strategies for the quality control and the development of new bioactive products of saffron in the future.

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Section: Antioxidant Activities Of 21 Batches Of Saffronmentioning

confidence: 99%

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

You

Zhong

et al. 2021

Journal of Chemistry

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“…Recently, the chromatographic fingerprint technique has been introduced as a tool to evaluate the quality of herbal samples or their derived products [10][11][12][13]. This technique has been accepted by the WHO and European Medicines Agency (EMEA) as a strategy for the quality assessment of herbal medicines [14] and gained more and more attention, because it emphases on the characterization of the complete sample composition.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic fingerprinting and quantitative analysis for the quality evaluation of Xinfeng capsule

Gao

Fang

Mei

2020

AChrom

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Abstract A simple, accurate and sensitive method of high performance liquid chromatography (HPLC) with diode array detector was established to identify Xinfeng capsules and systematically evaluated its quality, based on chromatographic fingerprint integrated with the similarity analysis, hierarchical cluster analysis and the quantitative analysis of multi-components by single marker (QAMS). In this study, 18 peaks were selected as the common peaks to evaluate the similarities among different batches (S1–S10) of Xinfeng capsules samples, which were manufactured in the First Affiliated Hospital of Anhui University of Chinese Medicine with a three-year span. Compared to control fingerprint, the similarities values for 10 batches of samples were more than 0.90. Moreover, by analyzing the reference of astragalus, the chromatogram of astragalus was developed, and 10 common peaks of astragalus were identified. More importantly, simultaneous quantification of three markers in Xinfeng capsule, including Calycosin-7-glucoside, calycosin and Formononetinaldehyde was performed, the three constituents showed good regression (R > 0.999) within linear ranges, and their recoveries were within the range of 97.6–101.5%. The validation results showed that the developed method was specific, accurate, precise and robust. This study demonstrated that the developed method offers an efficient, reliable and practical approach for systematic quality evaluation of Xinfeng capsule.

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Section: Introductionmentioning

confidence: 99%

“…The chromatographic fingerprinting approach has been accepted by the World Health Organization (WHO) to evaluate and QC of various traditional Chinese medicine (TCM) in recent years [1,2]. When the fingerprint method was adopted by the official pharmacopoeia, it became common practice Article Related Abbreviations: AQbD, analytical quality-by-design; CMA, critical method attribute; CMP, critical method parameter; DAD, diode-array detector; QbD, quality by design; TCM, traditional Chinese medicine to use a developed fingerprint method with fixed conditions.…”

Section: Introductionmentioning

confidence: 99%

Establishing the chromatographic fingerprint of traditional Chinese medicine standard decoction based on quality by design approach: A case study of Licorice

Zhang

Wang

Chen

et al. 2019

J of Separation Science

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A novel analytical quality by design approach for developing a chromatographic fingerprint was established for analyzing complex traditional Chinese medicine, using a licorice standard decoction as an example. Considering the characteristics of integrity and ambiguity, the resolution of eight common peaks, total peak number, capacity factor distributions, and peak purity were selected as potential critical method attributes for assessing the quality of the chromatographic fingerprint. A central composite design was used to evaluate the relationship between critical method attributes and critical method parameters, including column temperature, wavelength, flow rate, formic‐acid concentration, and gradient parameters. A standard probability method was employed to calculate the design space of the fingerprint analysis parameters and evaluate the robustness of the methodology. The optimized high‐performance liquid chromatography fingerprint conditions were acetonitrile and 0.1% formic acid water gradient elution (0‐5 min, 5–19% A; 5–10 min, 19% A; 10–50 min, 19–42% A; 50–54 min, 42–100% A; 54–60 min, 100% A), column temperature 25±5°C, detection wavelength 265 nm. The design space of fingerprint analytical method based on the analytical quality by design approach not only met the requirements of the fingerprint analysis, but also improved the robustness and applicability of the fingerprint method.

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Quantitative analysis combined with chromatographic fingerprint and antioxidant activities for the comprehensive evaluation of Compound Danshen Tablets

Cited by 21 publications

References 14 publications

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

Multivariate Statistical Analysis Uncovers Spectrum–Effect Relationship between HPLC Fingerprints and Antioxidant Activity of Saffron

Chromatographic fingerprinting and quantitative analysis for the quality evaluation of Xinfeng capsule

Establishing the chromatographic fingerprint of traditional Chinese medicine standard decoction based on quality by design approach: A case study of Licorice

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