Chromatographic fingerprinting and quantitative analysis for the quality evaluation of Xinfeng capsule

Abstract

<jats:title>Abstract</jats:title> <jats:p>A simple, accurate and sensitive method of high performance liquid chromatography (HPLC) with diode array detector was established to identify Xinfeng capsules and systematically evaluated its quality, based on chromatographic fingerprint integrated with the similarity analysis, hierarchical cluster analysis and the quantitative analysis of multi-components by single marker (QAMS). In this study, 18 peaks were selected as the common peaks to evaluate the similarities among different batches (S1–S10) of Xinfeng capsules samples, which were manufactured in the First Affiliated Hospital of Anhui University of Chinese Medicine with a three-year span. Compared to control fingerprint, the similarities values for 10 batches of samples were more than 0.90. Moreover, by analyzing the reference of astragalus, the chromatogram of astragalus was developed, and 10 common peaks of astragalus were identified. More importantly, simultaneous quantification of three markers in Xinfeng capsule, including Calycosin-7-glucoside, calycosin and Formononetinaldehyde was performed, the three constituents showed good regression (<jats:italic>R</jats:italic> &gt; 0.999) within linear ranges, and their recoveries were within the range of 97.6–101.5%. The validation results showed that the developed method was specific, accurate, precise and robust. This study demonstrated that the developed method offers an efficient, reliable and practical approach for systematic quality evaluation of Xinfeng capsule.</jats:p>

Jianpi Qingre Tongluo Decoction exerted an anti-inflammatory effect on AS by inhibiting the NONHSAT227927.1/JAK2/STAT3 axis

“…JQP has been used for the treatment of damp-heat syndrome of AS for more than 20 years, showing remarkable therapeutic effects . The HPLC-generated fingerprint has verified JQP's safety, stability, and efficacy in medical treatments [ 16 , 17 ]. JQP contributes to reducing AS inflammation and redox reactions [ 18 ], improving bone metabolism, relieving extra-articular symptoms, inhibiting platelet activation [ 19 ], and improving cardiac function [ 20 ].…”

Chinese herbal compound Xinfeng capsule inhibits cartilage extracellular matrix degradation and inflammation in osteoarthritis by modulating circ_0032131 and the miR-502-5p/TRAF2 axis

“…and Centipede(Chinese name Wugong; Scolopendra subspinipes mutilans L. Koch). Previous research has demonstrated that ngerprint graphs generated by the High E ciency Liquid Phosphorography (HPLC) can attest to XFC's employing reliable production procedures and stringent quality controls [13] . Several research has clari ed that XFC may alleviate OA patients' sensations of joint pain and the aberrant in ammatory immune response [14] .…”

Xinfeng capsule inhibits cartilage extracellular matrix degradation and inflammation in osteoarthritis by modulating circ_0032131 and miR-502-5p/TRAF2 axis

“…and Centipede(Chinese name Wugong; Scolopendra subspinipes mutilans L. Koch) at the ratio of 20:10:20:1. Previous research has demonstrated that ngerprint graphs generated by the High E ciency Liquid Phosphorography (HPLC) can attest to XFC's employing reliable production procedures and stringent quality controls 12 . Several research has clari ed that XFC may alleviate OA patients' sensations of joint pain and the aberrant in ammatory immune response 13 .…”