Quantitative Activity Measurements of Brown Adipose Tissue at 7 T Magnetic Resonance Imaging After Application of Triglyceride-Rich Lipoprotein 59Fe-Superparamagnetic Iron Oxide Nanoparticle

Jung, Caroline; Heine, Markus; Freund, Barbara; Reimer, Rudolph; Koziolek, Eva Jolanthe; Kaul, Michael; Kording, Fabian; Schumacher, Udo; Weller, Horst; Nielsen, Peter; Adam, Gerhard; Heeren, J.; Ittrich, Harald

doi:10.1097/rli.0000000000000235

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…According to our knowledge, it has not been researched before. In previous research, a noninvasive method to image BAT with superparamagnetic iron oxide nanoparticle (SPIO) has been used [ 18 ]. However, the applicability is limited without BAT-specific probes.…”

Section: Discussionmentioning

confidence: 99%

Targeted Molecular Magnetic Resonance Imaging Detects Brown Adipose Tissue with Ultrasmall Superparamagnetic Iron Oxide

Xiangxun

Liu

et al. 2018

BioMed Research International

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The peptide (CKGGRAKDC-NH2) specifically targets the brown adipose tissue (BAT). Here we applied this peptide coupled with polyethylene glycol (PEG)-coated ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to detect BAT in vivo by magnetic resonance imaging (MRI). The peptide was conjugated with PEG-coated USPIO nanoparticles to obtain targeted USPIO nanoprobes. Then the nanoprobes for BAT were evaluated in mice. T2⁎-weighted images were performed, precontrast and postcontrast USPIO nanoparticles. Finally, histological analyses proved the specific targeting. The specificity of targeted USPIO nanoprobes was observed in mice. The T2⁎ relaxation time of BAT in the targeted group decreased obviously compared to the controls (P<0.001). Prussian blue staining and transmission electron microscope confirmed the specific presence of iron oxide. This study demonstrated that peptide (CKGGRAKDC-NH2) coupled with PEG-coated USPIO nanoparticles could identify BAT noninvasively in vivo with MRI.

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Section: Discussionmentioning

confidence: 99%

Targeted Molecular Magnetic Resonance Imaging Detects Brown Adipose Tissue with Ultrasmall Superparamagnetic Iron Oxide

Xiangxun

Liu

et al. 2018

BioMed Research International

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“…Radioactively labeled superparamagnetic iron oxide nanoparticles [Triglyceride-Rich Lipoprotein (TRL)- 59 FE-SPIOs] have been embedded into a lipoprotein layer to study the uptake of the nanoparticles by different organs, such as the liver, blood, muscle, and BAT after either intravenous or intraperitoneal injection ( 159 ). Cold-exposed mice showed a significant decrease of T 2 * in BAT while this was not observed in the control group.…”

Section: Characterizing Bat Functionmentioning

confidence: 99%

Magnetic Resonance Imaging Techniques for Brown Adipose Tissue Detection

Junker

Branca

et al. 2020

Front. Endocrinol.

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Magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) methods can non-invasively assess brown adipose tissue (BAT) structure and function. Recently, MRI and MRS have been proposed as a means to differentiate BAT from white adipose tissue (WAT) and to extract morphological and functional information on BAT inaccessible by other means. Specifically, proton MR (1 H) techniques, such as proton density fat fraction mapping, diffusion imaging, and intermolecular multiple quantum coherence imaging, have been employed to access BAT microstructure; MR thermometry, relaxometry, and MRI and MRS with 31 P, 2 H, 13 C, and 129 Xe have shown to provide complementary information on BAT function. The purpose of the present review is to provide a comprehensive overview of MR imaging and spectroscopy techniques used to detect BAT in rodents and in humans. The present work discusses common challenges of current methods and provides an outlook on possible future directions of using MRI and MRS in BAT studies.

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“…Therefore, especially in the long term, all these methods are hampered by overlapping endogenous and exogenous iron. As of today, there is no such specific option for iron imaging and validation, except for the use of radioactive 59 Fe-labeled nanoparticles, in conjunction with single-photon-emission computed tomography, which is associated with the drawback of radiation exposure and decay of the radioactive isotope 59 Fe, as well as minor spatial resolution. ,− , 57 Fe, in contrast, is a stable isotope and free from temporal constraints for long-term detectability. By resolving the local distribution of both 57 Fe and 56 Fe within the tissue of interest in a quantitative isotope-specific manner, the combination of 57 Fe-ION MRI and mass spectrometry imaging expands the toolbox of MRI and provides a remedy for the major shortcoming of MRI in molecular imaging: the lack of specificity.…”

Section: Fe-ion For Combined Mri/la-icp-ms Cell Trackingmentioning

confidence: 99%

Introducing Specificity to Iron Oxide Nanoparticle Imaging by Combining ⁵⁷Fe-Based MRI and Mass Spectrometry

et al. 2019

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Iron oxide nanoparticles (ION) are highly sensitive probes for magnetic resonance imaging (MRI) that have previously been used for in vivo cell tracking and have enabled implementation of several diagnostic tools to detect and monitor disease. However, the in vivo MRI signal of ION can overlap with the signal from endogenous iron, resulting in a lack of detection specificity. Therefore, the long-term fate of administered ION remains largely unknown, and possible tissue deposition of iron cannot be assessed with established methods. Herein, we combine nonradioactive 57Fe-ION MRI with ex vivo laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) imaging, enabling unambiguous differentiation between endogenous iron (56Fe) and iron originating from applied ION in mice. We establish 57Fe-ION as an in vivo MRI sensor for cell tracking in a mouse model of subcutaneous inflammation and for assessing the long-term fate of 57Fe-ION. Our approach resolves the lack of detection specificity in ION imaging by unambiguously recording a 57Fe signature.

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Quantitative Activity Measurements of Brown Adipose Tissue at 7 T Magnetic Resonance Imaging After Application of Triglyceride-Rich Lipoprotein 59Fe-Superparamagnetic Iron Oxide Nanoparticle

Cited by 13 publications

References 27 publications

Targeted Molecular Magnetic Resonance Imaging Detects Brown Adipose Tissue with Ultrasmall Superparamagnetic Iron Oxide

Targeted Molecular Magnetic Resonance Imaging Detects Brown Adipose Tissue with Ultrasmall Superparamagnetic Iron Oxide

Magnetic Resonance Imaging Techniques for Brown Adipose Tissue Detection

Introducing Specificity to Iron Oxide Nanoparticle Imaging by Combining ⁵⁷Fe-Based MRI and Mass Spectrometry

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Quantitative Activity Measurements of Brown Adipose Tissue at 7 T Magnetic Resonance Imaging After Application of Triglyceride-Rich Lipoprotein 59Fe-Superparamagnetic Iron Oxide Nanoparticle

Cited by 13 publications

References 27 publications

Targeted Molecular Magnetic Resonance Imaging Detects Brown Adipose Tissue with Ultrasmall Superparamagnetic Iron Oxide

Targeted Molecular Magnetic Resonance Imaging Detects Brown Adipose Tissue with Ultrasmall Superparamagnetic Iron Oxide

Magnetic Resonance Imaging Techniques for Brown Adipose Tissue Detection

Introducing Specificity to Iron Oxide Nanoparticle Imaging by Combining 57Fe-Based MRI and Mass Spectrometry

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Product

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Introducing Specificity to Iron Oxide Nanoparticle Imaging by Combining ⁵⁷Fe-Based MRI and Mass Spectrometry