Quantitation of vitamin B6 in biological samples by isotope dilution mass spectrometry

Hachey, David L.; Coburn, Stephen P.; Brown, Laura T.; Erbelding, W.F.; DeMark, Bruce R.; Klein, Peter

doi:10.1016/0003-2697(85)90066-1

Cited by 18 publications

(6 citation statements)

References 12 publications

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“…The only assay reported in the literature dates back to 1985, when Hachey et al [39] used deuterated analogs of pyridoxine, pyridoxal, pyridoxamine, and pyridoxic acid to quantify these compounds and their phosphates in guinea pig liver, human urine, feces, and goat milk. In the last case, pyridoxal phosphate was found to be the main vitamer contributing almost 70% to total pyridoxine.…”

Section: Vitamin Bmentioning

confidence: 99%

Stable Isotope Dilution Assays in Vitamin Analysis–A Review of Principles and Applications

Rychlik¹

2011

Fortified Foods With Vitamins

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Section: Vitamin Bmentioning

confidence: 99%

Stable Isotope Dilution Assays in Vitamin Analysis–A Review of Principles and Applications

Rychlik¹

2011

Fortified Foods With Vitamins

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“…Addition of an isotopic standard to the sample at the early stages of the analytical method freezes the concentration information as an isotopic ratio that generally is immune to analyte losses during subsequent isolation and derivatization steps (Hachey et al, 1985).…”

Section: Pantothenic Acidmentioning

confidence: 99%

Quantification of Pantothenic Acid and Folates by Stable Isotope Dilution Assays

Rychlik

Freisleben

2002

Journal of Food Composition and Analysis

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“…Trichloroacetic acid extraction has been applied to clinical studies (Coburn and Mahuren 1983, Hachey et al 1985, Shephard et al1987, Shephard et al 1989, Mahuren and Coburn 1997, and to human milk (Hamaker et al 1985) and food and feed analysis (van Schoonhoven et al 1994) as well as measuring the sugar-derivatives of pyridoxine (Ink et al 1986, Sierra andVidal-Valverde 1997). As the phosphate ester and glucosidic bond were not hydrolyzed by TCA the extraction in lowered or ambient temperature, this extraction enabled the determination of free, phosphorylated and glycosylated compounds.…”

Section: Trichloroacetic Acid (Tca)mentioning

confidence: 99%

“…The few published papers are mainly limited to the use of UV detection after an ion-exchange purification of extracts (Wong 1978), in the fraction collection step prior to mass spectrometry (Hachey et al 1985) or to the determination of vitamin B 6 in the vitamin preparations or enriched foods (Agostini and Godoy 1997). The wavelength of a conventional UV detector was set at 254 nm for multivitamin capsule preparations (Amin andReusch 1987, Maeda et al 1989) and 280-290 nm for the commercial multivitamin preparations (Callmer and Davies 1974, Lam et al 1984, Bühnert 1988, Hauenstein 1990, Iwase 1992).…”

Section: Detectionmentioning

confidence: 99%

“…Rigorous pretreatment is also needed to remove nonvolatile compounds and water from the extract. Derivatization procedures include formation of acetyl (Hachey et al 1985) or trifluoroacetyl (Lim et al 1982) derivatives. The use of an electron-capture detector enabled a low detection limit (10pg) for all three free vitamer (Lim et al 1982).…”

Section: Gas Chromatography and Massmentioning

confidence: 99%

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HPLC analysis of vitamin B6 in foods

Ollilainen¹

1999

AFSci

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The objective of this work was to evaluate the methods for determination of vitamin B6 in foods. To achieve this, the literature review focused on sample treatment and liquid chromatographic analysis of vitamin B6 related compounds. In the experimental part, the chosen sample pretreatment and the high-performance liquid chromatographic (HPLC) method were validated, and used to produce vitamin B6 data on various food items commonly consumed in Finland. The main emphasis of the sample treatment was on the extraction efficiency and the maintenance of the original concentration profile of the vitamers. Several acid extraction procedures were tested for this purpose. Perchloric acid was chosen as the extraction agent. Routine food analysis was then performed using dilute ice-cold perchloric acid extraction followed by an internally standardized ion-paired reversed-phase liquid chromatography. Food samples were hydrolyzed with takadiastase and alkaline phosphatase enzymes, phosphorylated and glycosylated vitamers were quantitated before and after the enzymatic digestion. This procedure enabled the extraction of vitamin B6 compounds in their intact forms, and the measurement of free, phosphorylated and glycosylated forms. The maintenance of the concentration profile of the vitamers was verified by using 14C -labeled pyridoxal 5'-phosphate in the examination of the extraction procedure. The extraction efficiency and laboratory performance were confirmed by interlaboratory studies. Up-to-date data on vitamin B6 content of about fifty common food items was produced. The data includes the results from meat and poultry, fish and fish product, dairy product, cereal and vegetable, and ready-to-eat food samples. Free and phosphorylated vitamin B6 compounds were measured in all food groups, and the glycosylated vitamer fraction was analyzed in all plant-derived foods. The results obtained in this work showed that vitamin B6 content of nearly all foods of plant origin was mainly comprised of glycosidically bound pyridoxine derivatives. These bound analytes are normally not taken into account in traditional analytical methods, and food composition tables lack the data of glycosylated pyridoxine. The role of the glycosylated pyridoxines need to be clarified in terms of their analytical and physiological nature. If, as it is currently assumed, the availability of the bound forms is limited for humans, the role of vegetables, cereals and other foods of plant origin as a source of vitamin B6, as well as the analytical methods should be reassessed.;

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Quantitation of vitamin B6 in biological samples by isotope dilution mass spectrometry

Cited by 18 publications

References 12 publications

Stable Isotope Dilution Assays in Vitamin Analysis–A Review of Principles and Applications

Stable Isotope Dilution Assays in Vitamin Analysis–A Review of Principles and Applications

Quantification of Pantothenic Acid and Folates by Stable Isotope Dilution Assays

HPLC analysis of vitamin B6 in foods

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