Quantification of Pantothenic Acid and Folates by Stable Isotope Dilution Assays

Rychlik, Michael; Freisleben, Achim

doi:10.1006/jfca.2002.1081

Cited by 20 publications

(8 citation statements)

References 19 publications

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“…In order to achieve this distinction, chromatographic methods are required. While a number of different detectors such as UV(-DAD), fluorescence, electrochemical and mass spectrometric detectors have been reported in conjunction with high performance liquid chromatography (HPLC) (Pfeiffer et al, 1997; Bagley and Selhub, 2000; Ndaw et al, 2001; Rychlik and Freisleben, 2002; Doherty and Beecher, 2003; Freisleben et al, 2003a; Zhang et al, 2003; Nelson et al, 2004; Fazili et al, 2005; Garratt et al, 2005; Zhang et al, 2005; De Brouwer et al, 2007; Patring and Jastrebova, 2007; Gutzeit et al, 2008), (Ultra-) HPLC coupled to tandem mass spectrometry [(U)HPLC-MS/MS] has become the de facto standard for the sensitive and selective determination of folates (De Brouwer et al, 2010). UHPLC-MS/MS has also been employed for the two-dimensional characterization of folate content in potato tubers (Van Daele et al, 2014).…”

Section: Quantitative Analysis Of Folates In Plant Samples a Prerequmentioning

confidence: 99%

Folates in Plants: Research Advances and Progress in Crop Biofortification

et al. 2017

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Folates, also known as B9 vitamins, serve as donors and acceptors in one-carbon (C1) transfer reactions. The latter are involved in synthesis of many important biomolecules, such as amino acids, nucleic acids and vitamin B5. Folates also play a central role in the methyl cycle that provides one-carbon groups for methylation reactions. The important functions fulfilled by folates make them essential in all living organisms. Plants, being able to synthesize folates de novo, serve as an excellent dietary source of folates for animals that lack the respective biosynthetic pathway. Unfortunately, the most important staple crops such as rice, potato and maize are rather poor sources of folates. Insufficient folate consumption is known to cause severe developmental disorders in humans. Two approaches are employed to fight folate deficiency: pharmacological supplementation in the form of folate pills and biofortification of staple crops. As the former approach is considered rather costly for the major part of the world population, biofortification of staple crops is viewed as a decent alternative in the struggle against folate deficiency. Therefore, strategies, challenges and recent progress of folate enhancement in plants will be addressed in this review. Apart from the ever-growing need for the enhancement of nutritional quality of crops, the world population faces climate change catastrophes or environmental stresses, such as elevated temperatures, drought, salinity that severely affect growth and productivity of crops. Due to immense diversity of their biochemical functions, folates take part in virtually every aspect of plant physiology. Any disturbance to the plant folate metabolism leads to severe growth inhibition and, as a consequence, to a lower productivity. Whereas today's knowledge of folate biochemistry can be considered very profound, evidence on the physiological roles of folates in plants only starts to emerge. In the current review we will discuss the implication of folates in various aspects of plant physiology and development.

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Section: Quantitative Analysis Of Folates In Plant Samples a Prerequmentioning

confidence: 99%

Folates in Plants: Research Advances and Progress in Crop Biofortification

et al. 2017

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“…The folacin complex includes folic acid (pteroylglutamic acid), dihydrofolic acid, tetrahydrofolic acid (H 4 folic acid), 5 -formyl -H 4 folic acid, 5 -methyl -H 4 folic acid, and their derivatives characterized by chains containing 2 -8 glutamic acid residues [1,14] . The determination of the total B 9 content usually requires the release of folates from the food matrix, enzymatic deconjugation (folic acid conjugase) of the polyglutamates to the monoglutamate forms, and a fi nal cleanup step [4,5] .…”

Section: Extraction and Cleanup With Conventional Methods Of Analysismentioning

confidence: 99%

“…In the early 2000s, the ESI source was applied for the fi rst time to pantothenic acid detection [42] and to distinguish the several vitamers of folacin complex in both the positive and negative ionization modes [43,44] . In particular, stable isotope dilution LC -MS/MS assay was devised as a reference procedure for validating LC methods using UV or fl uorescence detection ( [14,45] ) (see also Chapter 1 ). ESI, successfully applied to the analysis of B 12 in some fortifi ed foods [46] , is a promising LC detection mode for the total determination of this vitamin and for the direct characterization of its vitamers naturally occurring in foods.…”

Section: Lc -Ms In Multianalyte Confi Rmation Analysesmentioning

confidence: 99%

Multimethod for Water‐Soluble Vitamins in Foods by Using LC–MS

Gentili

Caretti

2011

Fortified Foods With Vitamins

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“…The benefits of stable isotope dilution methodology have also been reported for other odorants (Rychlik & Bosset, 2001), mycotoxins (Rychlik & Schieberle, 2001), or vitamins (Rychlik & Freisleben, 2002).…”

Section: Introductionmentioning

confidence: 95%