Quantitation of vancomycin and its crystalline degradation product (CDP-1) in human serum by high performance liquid chromatography

Backes, D.; Aboleneen, Hoda; Simpson, J.

doi:10.1016/s0731-7085(97)00140-4

Cited by 54 publications

(44 citation statements)

References 24 publications

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“…Solid-phase extraction, as used by Greene et al (1987), was replaced by a less timeconsuming method of sample preparation. The LLOQ found in plasma conforms to other LLOQs reported for vancomycin in bioanalytical assays using HPLC with UV detection (Farin et al, 1998;Furuta et al, 2000) or was even lower (Li et al, 1995;Greene et al, 1987;Backes et al, 1998;Demotes-Mainard et al, 1994;Hu et al, 1990;Luksa and Marusic, 1995). More sensitive methods have been reported using tandem mass spectrometry for detection (Cass et al, 2001;Shibata et al, 2003).…”

Section: Resultssupporting

confidence: 82%

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Plock¹,

Buerger²,

Kloft³

2005

Biomed. Chromatogr.

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Vancomycin is a glycopeptide antibiotic approved for the treatment of serious infections or patients allergic to beta-lactams. A rapid HPLC assay using UV detection for the determination in microdialysate and human plasma was developed. After sample preparation, using methanol and trichloroacetic acid for plasma and water for microdialysate, 20 microL were injected and separated on a RP(18) column. Overall, the assay exhibited good precision and accuracy. The diffusion properties of vancomycin investigated in in vitro microdialysis experiments revealed an unfavourable concentration dependence avertable by keeping a constant pH using phosphate buffer as perfusate. The mean relative recoveries were 27.8% [coefficient of variation (CV) 11.1%] and 33.2% (CV 8.3%) for retrodialysis and recovery experiments, respectively. Following characterization of vancomycin in in vitro microdialysis, the developed setting is suitable for application in (pre-)clinical studies.

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Section: Resultssupporting

confidence: 82%

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Plock¹,

Buerger²,

Kloft³

2005

Biomed. Chromatogr.

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“…The method was validated with regard to its specificity, linearity, accuracy and sensitivity (within and between days) [20][21][22][23][24][25]. for research purpose of VCM formulation in systemic use.…”

Section: Methods Validationmentioning

confidence: 99%

A Simple and Modified Method Development of Vancomycin Using High Performance Liquid Chromatography

Chauhan¹,

Bhatt²

2015

J Chromatogr Sep Tech

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“…The validated analytic HPLC method used in this study was adapted from the published methods of Backes et al [3]. The system consisted of a Varian Prostar 240 Solvent Delivery Module, Varian Prostar 430 Autosampler, and Varian Prostar 335 Photodiode Array Detector (Agilent Technologies, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

The In Vitro Elution Characteristics of Vancomycin from Tendons

Grayson

Grant

Dukie

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Infection after ACL reconstruction is uncommon but catastrophic. Prophylactic graft saturation in vancomycin reportedly reduces infection rates. Questions/purposes We characterized vancomycin elution from soaked tendons. Specifically, the effect of rinsing was studied. We also determined how vancomycin concentration in the soak solution and tendon dimension influenced this elution rate, and examined whether the vancomycin amount released was lower than osteoblast and chondroblast toxic concentrations. Methods Bovine tendons were wrapped in sterile gauze swabs presoaked with 5-, 2.5-, or 1.25-mg/mL vancomycin solutions. After 10 minutes, rinsed and unrinsed tendons were placed in 100 mL agitated 37°C phosphate-buffered saline (PBS). One-milliliter samples taken at 10 minutes and 1, 6, 12, 24, and 72 hours were analyzed by highperformance liquid chromatography. ResultsThe maximum elution rate occurred between 10 minutes and 1 hour, with no lag between experiment initiation and drug appearance in the solution. Rinsing affected the initial amount in solution but had little influence on drug release after 10 minutes. Vancomycin diffusion rates were dependent on soak solution concentration at all sampling intervals. The vancomycin amount released or eluted did not increase after the 1-hour interval. At 24 hours, concentrations were 45 ± 12, 16 ± 1, and 9 ± 3 lg/mL for the 5-, 2.5-, and 1.25-mg/mL solutions, respectively. Higher elution rates were observed in largervolume tendons. Conclusions Soaked tendon grafts can act as reservoirs for vancomycin, with the amount released and elution profile dependent on rinsing, tendon volume, and soak solution concentration. Vancomycin elution was lower than previously reported osteoblast and chondroblast toxicity concentrations and above the minimum inhibitory concentration for Staphylococcus. Clinical Relevance Presoaking ACL reconstruction autografts with vancomycin may reduce the risk of ACL reconstruction infection without the risk of local or general toxicity.

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Quantitation of vancomycin and its crystalline degradation product (CDP-1) in human serum by high performance liquid chromatography

Cited by 54 publications

References 24 publications

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

A Simple and Modified Method Development of Vancomycin Using High Performance Liquid Chromatography

The In Vitro Elution Characteristics of Vancomycin from Tendons

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