Quantitation of talinolol and other β‐blockers by capillary electrophoresis for in vitro drug absorption studies

Abstract

A capillary zone electrophoresis method is described for the enantioseparation of talinolol using heptakis(2,3-diacetyl-6-sulfo)-beta-cyclodextrin (HDAS-beta-CD) as a chiral selector. After liquid-liquid extraction of talinolol from physiological solution, electrokinetic injection was employed to improve the sensitivity. The use of a coated capillary was necessary to achieve stable and reproducible enantioseparations. A baseline separation of the talinolol enantiomers was achieved in less than 10 min using 100 mM phosphate solution as background electrolyte and pH 3.5, at the presence of 3.0 mM HDAS-beta-CD and at 20 degrees C. In addition, this analytical condition proved to be useful for the enantioseparation of a number of other beta-blocking agents such as alprenolol, atenolol, bisoprolol, celiprolol, metipranolol, oxprenolol, and sotalol. For determining talinolol, the method could be validated in terms of precision, accuracy and linearity, and was found to be suitable in determination of talinolol enantiomers in highly diluted samples obtained from in vitro experiments.

The role of cyclodextrins in chiral capillary electrophoresis

“…25 Pharmaceuticals [14], tolerodine and analogs [128], omeprazole [130], azabicyclo drug Weak basic model compounds [13], deprenyl [62], alkaloids [124], talinolol [137], labetalol [138] The multicharged basic CDs generate anodic migration, because the wall-adsorbed selectors change the direction of the EOF [53].…”

Liquid chromatographic–electrospray tandem mass spectrometric determination of bisoprolol in human plasma

“…Prior to the prevalent use of LC-MS techniques, various analytical methods, such as capillary electrophoresis (Awadallah et al, 2003), gas chromatography (Kim et al, 2001) and HPLC with an ultraviolet (Zhang et al, 2003;Ruiz-Angel et al, 2002;Modamio et al, 1996), fluorescence (Buhring and Garbe, 1986;Eastwood et al, 1990;Suzuki et al, 1993) or diode array detector (Caudron et al, 2004), have been developed to determine bisoprolol in formulation and biological samples.…”

Hydrophobicity‐aided potentiometric detection of catecholamines, beta‐agonists, and beta‐blockers in a mixed‐solvent capillary electrophoresis system

“…In addition, the low acidic background electrolyte significantly decreased the interactions of residual silanol groups on the walls of the fused-silica capillary with the fully protonated drugs [35,41]. The acidic running electrolyte solution with low pH (2.0 -3.5) was frequently applied in non-chiral [36, 42 -44] and enantioselective [38] separation of catecholamines, beta-blockers, and beta-agonists with CE systems employing uncoated [36,42,43], coated [44,45], C18 covalently bonded [46] fused-silica capillaries or using titanium dioxide nanoparticles as additive in BGE [36]. Symbols: tm -migration time (mean l SD; n = 5); Rs -resolution of two subsequent analytes; lact -apparent solute mobility; leff -effective solute mobility; D -diffusion coefficient; pKa -first acidic ionisation constant; log P -n-octanol -water partition coefficient; MW -molecular weight; HBA -number of hydrogen bond acceptors in analyte molecule.…”