Liquid chromatographic–electrospray tandem mass spectrometric determination of bisoprolol in human plasma

Abstract

An analytical method for the determination of bisoprolol in human plasma has been developed based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte and internal standard (IS) diphenhydramine were cleaned up by protein precipitation with acetonitrile, reconstituted in mobile phase and separated by reversed-phase high-performance liquid chromatography (HPLC) using methanol:10 mm ammonium acetate:formic acid (70:30:0.1 v/v/v) as mobile phase. Detection was carried out by multiple reaction monitoring (MRM) on an LC-MS/MS system and was completed within 2.5 min. The assay was linear over the range 0.5-100 ng/mL with a limit of quantitation (LOQ) of 0.5 ng/mL. The intra- and inter-day precision levels were within 5.54 and 9.95%, respectively, while the accuracy was in the range 89.4-113%. This method has been utilized in a pharmacokinetic study, where healthy volunteers were treated with an oral dose of 5 mg bisoprolol.

Simultaneous determination of bisoprolol and hydrochlorothiazide in human plasma by HPLC coupled with tandem mass spectrometry

“…Several chromatographic methods have been reported for the analysis of either BISO or HCTZ alone in human plasma; such methods have included HPLC coupled with UV [5][6][7] or fluorescence [8], diode array [9], LC-MS [10][11][12] or with tandem LC-MS/MS detection [13][14][15][16][17][18][19].…”

A New Liquid Chromatography‐Tandem Mass Spectrometry Method for Determination of Bisoprolol in Human Plasma Samples

“…Also, Liu et al [7] reported an accuracy ranged from 89.4%–113%, employing a precipitation with acetonitrile procedure for plasma sample preparations.…”

Preparation of Biological Samples Containing Metoprolol and Bisoprolol for Applying Methods for Quantitative Analysis

“…Bisoprolol was also separated from the proteins in human plasma by protein precipitation with acetonitrile, then analyzed by liquid chromatography coupled with mass spectrometry using diphenhydramine as internal standard. This technique applied for biological sample preparation provided a good recovery of the analyte, with values of 92.7±8.1, 94.8±4.9 and 95.9±3% at concentration of 1, 10 and 80 ng/mL [12]. Protein precipitation technique was applied for the separation of bisoprolol in human plasma, with a recovery rate of about 94.03, 100.3 and 96.29% for 1.5, 40 and 80 ng/mL of bisoprolol, respectively.…”

“…These methods include liquid chromatography coupled with mass spectrometry (LC-MS/MS) [7][8][9][10][11][12][13][14][15][16][17], liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS) [18,19], gas chromatography coupled with mass spectrometry (GC-MS) [3,[20][21][22], high performance liquid chromatography (HPLC) [23,24], high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) [25,26], high performance liquid chromatography with fluorescence detection (HPLC-FLD) [2,27,28], high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) [29], reversed-phase ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS) [30].…”