Quantitation of meiosis activating sterols in human follicular fluid using HPLC and photodiode array detection

Baltsen, M; Byskov, Anne Grete

doi:10.1002/(sici)1099-0801(199910)13:6<382::aid-bmc896>3.0.co;2-d

Cited by 28 publications

(19 citation statements)

References 19 publications

(17 reference statements)

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“…Cleansed ovaries were dried briefly on a paper tissue, placed in a glass vial, weighed, and freeze-dried. After drying, the ovaries were weighed again and subjected to organic extraction according to a previously reported method [15]. Dry weight:wet weight ratios were fairly constant (Ϯ5%) for similarly treated ovaries.…”

Section: Ovarian Stimulation and Tissue Preparation For In Vivo Studiesmentioning

confidence: 99%

“…However, this rather operational level may remain largely dependent on the protocol for introduction of the extremely lipophilic molecule into the culture medium, but to our knowledge, no data regarding actual concentrations in the resulting media have yet been reported. The physiological concentration of FF-MAS plus T-MAS in human follicular fluid is approximately 1 g/ml [15], but so far, no data are available regarding mouse ovaries or mouse follicular fluid.…”

Section: Introductionmentioning

confidence: 99%

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Gonadotropin-Induced Accumulation of 4,4-Dimethylsterols in Mouse Ovaries and Its Temporal Relation to Meiosis1

Baltsen

2001

Biology of Reproduction

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The resumption of oocyte meiosis is triggered by a number of 4,4-dimethylsterols termed meiosis-activating sterols (MAS). The levels of meiosis active (follicular fluid [FF]-MAS and bull testes [T]-MAS) and inactive (lanosterol) 4,4-dimethylsterols, free cholesterol, and progesterone were determined in gonadotropin-primed prepubertal mouse ovaries in vivo by high-performance liquid chromatography. Ovaries responded to an ovulatory stimulation by increasing their content of 4,4-dimethylsterols but not of free cholesterol. The ovarian 4,4-dimethylsterol response was followed with regard to time and dose-response to the gonadotropins and the resumption of meiosis was evaluated using histologic sections. All 4,4-dimethylsterols accumulated in a time-dependent manner in gonadotropin-primed mice after a subsequent stimulation with hCG. The peak of 4,4-dimethylsterol accumulation appeared postmeiotically but coincided roughly with ovulation, and the resumption of meiosis was triggered when the intraovarian level of MAS was <20% of its maximum. The ovarian accumulation of progesterone preceded the 4,4-dimethylsterol accumulation. The FF-MAS accumulation displayed a dose-response maximum with respect to hCG, and a variation of the follicular priming regime revealed that, in contrast to progesterone production, 4,4-dimethylsterol accumulation is dependent on previous follicular growth beyond the gonadotropin-dependent stage. The FF-MAS was not liberated from esterified stores during the accumulatory response and appeared to be synthesized de novo from a precursor (or precursors) metabolically upstream to lanosterol. The data remain inconsistent with a model in which MAS is regarded as the physiological trigger of meiosis. The 4,4-dimethylsterol accumulation is suggested to influence maturation processes by affecting membrane sterol composition.

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Section: Ovarian Stimulation and Tissue Preparation For In Vivo Studiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gonadotropin-Induced Accumulation of 4,4-Dimethylsterols in Mouse Ovaries and Its Temporal Relation to Meiosis1

Baltsen

2001

Biology of Reproduction

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“…Follicular fluid may also act as a strong buffer of free radicals (25,26). Furthermore, follicular fluid contains many proteins and other factors that may be beneficial to human oocytes, such as transport proteins, intercellular signals, and hormones (23,24,(27)(28)(29)(30)(31)(32). Although some or all of the above factors could have positive effects on oocyte quality, we speculate in this work that one of the fundamental differences between follicular fluid and artificial culture media is the pH of the two fluids.…”

Section: Discussionmentioning

confidence: 99%

Naples, Italy

Wilding¹,

Singer²,

Fehr

et al. 2006

J Assist Reprod Genet

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“…Total sterols from liver, brain and testes were extracted as previously described [19,20]. Briefly, frozen tissues were freeze-dried and lipids were extracted in 75% n-heptane-25% isopropanol (v/v).…”

Section: Sterol Extraction and Lc-ms Analysismentioning

confidence: 99%

Adaptation of cholesterol synthesis to fasting and TNF-α: Profiling cholesterol intermediates in the liver, brain, and testis

Tacer

Pompon

Rozman

2010

The Journal of Steroid Biochemistry and Molecular Biology

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Quantitation of meiosis activating sterols in human follicular fluid using HPLC and photodiode array detection

Cited by 28 publications

References 19 publications

Gonadotropin-Induced Accumulation of 4,4-Dimethylsterols in Mouse Ovaries and Its Temporal Relation to Meiosis1

Gonadotropin-Induced Accumulation of 4,4-Dimethylsterols in Mouse Ovaries and Its Temporal Relation to Meiosis1

Naples, Italy

Adaptation of cholesterol synthesis to fasting and TNF-α: Profiling cholesterol intermediates in the liver, brain, and testis

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