Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer

Žáček, Petr; Bukowski, Michael R.; Rosenberger, Thad A.; Picklo, Matthew J.

doi:10.1194/jlr.d070656

Cited by 25 publications

(25 citation statements)

References 51 publications

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“…Only the sum compositions (total chain length and the total number of unsaturations) of phospholipids were determined in this study given the employed analytical method (Liebisch et al 2013). These sum compositions comprise lipid species with different FA compositions (Zacek et al 2016). These findings will be discussed in more detail below.…”

Section: Resultsmentioning

confidence: 99%

Serum lipidome analysis of healthy beagle dogs receiving different diets

et al. 2019

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IntroductionFood and dietary ingredients have significant effects on metabolism and health.ObjectiveTo evaluate whether and how different diets affected the serum lipidomic profile of dogs.MethodsSixteen healthy beagles were fed a commercial dry diet for 3 months (control diet). After an overnight fasting period, a blood sample was taken for serum lipidomic profile analysis, and each dog was then randomly assigned to one of two groups. Group 1 was fed a commercial diet (Diet 1) and group 2 was fed a self-made, balanced diet supplemented with linseed oil and salmon oil (Diet 2) for 3 months. After an overnight fasting period, a blood sample was taken from each dog. Serum cholesterol and triacylglycerol analyses were performed and the serum lipidomic profiles were analyzed using targeted liquid chromatography–mass spectrometry.ResultsDogs fed the supplemented self-made diet (Diet 2) had significantly higher omega-3 fatty acid-containing lipids species and significantly lower saturated and mono- and di-unsaturated lipid species. Concentrations of sphingosine 1-phosphate species S1P d16:1 and S1P d17:1 were significantly increased after feeding Diet 2.ConclusionThis study found that different diets had significant effects on the dog’s serum lipidomic profile. Therefore, in studies that include lipidomic analyses, diet should be included as a confounding factor.Electronic supplementary materialThe online version of this article (10.1007/s11306-019-1621-3) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Serum lipidome analysis of healthy beagle dogs receiving different diets

et al. 2019

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“…The fragment ions from the loss of the phosphoethanolamine group (-141) and fatty acyl chain were selected as Q3 ion for PE (LPE and PE) and neutral lipids (DAG and TAG), respectively [20]. For LPC, PC, plasmanyl PC, plasmenyl PC, and SM species, the transition from the protonated molecular ion ([M+H] + ) to the polar head fragment ion ( m/z 184, phosphocholine) was recorded for the quantitation [21]. Regarding DAG and TAG species, the transition from the ammoniated molecular ion ([M+NH 4 ] + ) to the fragment ions from the loss of fatty acyl chain were recorded (S1 Fig).…”

Section: Resultsmentioning

confidence: 99%

Plasma sphingomyelins increase in pre-diabetic Korean men with abdominal obesity

Park

Shon

et al. 2019

PLoS ONE

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Abdominal or visceral obesity is a well-known risk factor for metabolic diseases. However, whether abdominal obesity significantly affects plasma lipid profile during the development of type 2 diabetes has not been fully elucidated. We investigated the differences in plasma lipid concentrations in 63 participants categorized into six groups (middle-aged Korean men); Normal, Pre-diabetes (pre-DM), and Diabetes mellitus (DM) with or without abdominal obesity (AO or lean). The lipidomic profiles were determined by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sphingomyelin (SM) levels in plasma were significantly higher in the pre-DM with AO than in pre-DM with lean ( p = 0.021). SM concentrations correlated positively with waist-to-hip ratio (WHR) ( r = 0.256, p = 0.044), cholesteryl ester (CE) ( r = 0.483, p < 0.0001), ceramide ( r = 0.489, p < 0.0001) and plasmanyl phosphatidylcholine (PC) ( r = 0.446, p < 0.0001). The present study found that pre-diabetic patients with AO were characterized by increased plasma concentrations of SM. Plasma SM levels in individuals with AO may be an early prognostic biomarker to better predict the progression toward type 2 diabetes and metabolic syndrome.

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“…Indeed, a cursory analysis of data acquired from human plasma extracts using our approach reveals an underlying complexity that may not be adequately captured by other approaches. Given that a recent analysis of the NIST Standard Reference Material for human plasma (SRM1950) identified and quantified ~70 PC lipids, 37 many of which were unsaturated, it is reasonable to assume that including double bond regioisomers could expand this by a factor of two or more. Consistent with other data-independent strategies, the datasets could be re-interrogated to test evolving hypotheses of the lipidome without having to re-analyze the samples.…”

Section: Discussionmentioning

confidence: 99%

Online Ozonolysis Combined with Ion Mobility-Mass Spectrometry Provides a New Platform for Lipid Isomer Analyses

et al. 2017

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One of the most significant challenges in contemporary lipidomics lies in the separation and identification of lipid isomers that differ only in site(s) of unsaturation or geometric configuration of the carbon-carbon double bonds. While analytical separation techniques including ion mobility spectrometry (IMS) and liquid chromatography (LC) can separate isomeric lipids under appropriate conditions, conventional tandem mass spectrometry cannot provide unequivocal identification. To address this challenge, we have implemented ozone-induced dissociation (OzID) in-line with LC, IMS and high resolution mass spectrometry. Modification of an IMS-capable quadrupole time-of-flight mass spectrometer was undertaken to allow the introduction of ozone into the high-pressure trapping ion funnel region preceding the IMS cell. This enabled the novel LC-OzID-IMS-MS configuration where ozonolysis of ionized lipids occurred rapidly (10 ms) without prior mass-selection. LC-elution time alignment combined with accurate mass and arrival time extraction of ozonolysis products facilitated correlation of precursor and product ions without mass-selection (and associated reductions in duty cycle). Unsaturated lipids across 11 classes were examined using this workflow in both positive and negative ion modalities and in all cases the positions of carbon-carbon double bonds were unequivocally assigned based on predictable OzID transitions. Under these conditions geometric isomers exhibited different IMS arrival time distributions and distinct OzID product ion ratios providing a means for discrimination of cis/trans double bonds in complex lipids. The combination of OzID with multidimensional separations shows significant promise for facile profiling of unsaturation patterns within complex lipidomes including human plasma.

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Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer

Cited by 25 publications

References 51 publications

Serum lipidome analysis of healthy beagle dogs receiving different diets

Serum lipidome analysis of healthy beagle dogs receiving different diets

Plasma sphingomyelins increase in pre-diabetic Korean men with abdominal obesity

Online Ozonolysis Combined with Ion Mobility-Mass Spectrometry Provides a New Platform for Lipid Isomer Analyses

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