Serum lipidome analysis of healthy beagle dogs receiving different diets

Abstract

IntroductionFood and dietary ingredients have significant effects on metabolism and health.ObjectiveTo evaluate whether and how different diets affected the serum lipidomic profile of dogs.MethodsSixteen healthy beagles were fed a commercial dry diet for 3 months (control diet). After an overnight fasting period, a blood sample was taken for serum lipidomic profile analysis, and each dog was then randomly assigned to one of two groups. Group 1 was fed a commercial diet (Diet 1) and group 2 was fed a self-made, balanced diet supplemented with linseed oil and salmon oil (Diet 2) for 3 months. After an overnight fasting period, a blood sample was taken from each dog. Serum cholesterol and triacylglycerol analyses were performed and the serum lipidomic profiles were analyzed using targeted liquid chromatography–mass spectrometry.ResultsDogs fed the supplemented self-made diet (Diet 2) had significantly higher omega-3 fatty acid-containing lipids species and significantly lower saturated and mono- and di-unsaturated lipid species. Concentrations of sphingosine 1-phosphate species S1P d16:1 and S1P d17:1 were significantly increased after feeding Diet 2.ConclusionThis study found that different diets had significant effects on the dog’s serum lipidomic profile. Therefore, in studies that include lipidomic analyses, diet should be included as a confounding factor.Electronic supplementary materialThe online version of this article (10.1007/s11306-019-1621-3) contains supplementary material, which is available to authorized users.

Metabolomics shows the Australian dingo has a unique plasma profile

“…For instance, in several domestic dog breeds, the difference in plasma lipidome is influenced by diet under both controlled and uncontrolled dietary experiments (Lloyd et al, 2017, Boretti et al, 2020. In this study, we detected significant metabolite differences between dingoes and domestic dog breeds using a non-targeted plasma metabolome technique.…”

“…Blood metabolite profile between individuals and species can be shaped by genetic and by environmental factors including dietary intake, physical condition and gut microflora (Nicholson et al, 2011, Suhre and Gieger, 2012, Kettunen et al, 2012, Fujisaka et al, 2018). For instance, in several domestic dog breeds, the difference in plasma lipidome is influenced by diet under both controlled and uncontrolled dietary experiments (Lloyd et al, 2017, Boretti et al, 2020). In this study, we detected significant metabolite differences between dingoes and domestic dog breeds using a non-targeted plasma metabolome technique.…”

The Erythrocyte Membrane Lipidome of Healthy Dogs: Creating a Benchmark of Fatty Acid Distribution and Interval Values

“…The overall fatty acid distribution reported in Table 1 and Figures 2 , 3 highlights that in the dog erythrocyte membrane lipidome the omega-6 PUFA content is prevalent followed by SFA and MUFA. It is worth underlining that omega-3 fatty acids are present in minimal concentrations, marking the difference with human lipidome ( 11 , 18 , 24 , 30 – 32 ). Moreover, in healthy dogs the SFAs, stearic and palmitic acids, and the omega-6 PUFAs, arachidonic, and linoleic acids, sum up to nearly the 94% of the total membrane RBC fatty acids, as already observed in other mammalian species ( 24 ).…”

“…By these premises, we considered whether analogous data are reported in domestic animals, finding only a few studies on plasma or other tissues (brain, sperm) and on correlations between dietary uptake and lipidomic profiles of dogs (30)(31)(32)(33)(34). Limited studies presented a small number of samples (35,36), focusing for example on PUFA (omega-6 and omega-3), and not deepening the different fatty acid families, which are instead important for molecular contribution to membrane homeostasis (37).…”

Safety of Dietary Camelina Oil Supplementation in Healthy, Adult Dogs

“…Current data in dogs is contradictory as to whether high-fat diets are associated with an increase or decrease in serum cholesterol concentrations, though fat type, rather than total crude fat inclusion, may play a larger role in serum cholesterol concentrations [26][27][28][29]. In the present study, there was a significant change in cholesterol concentrations for treatment and week interaction, where OLA was significantly higher at week 10 than pre-study values and FLX was significantly lower at week 10 than pre-study values, though no significance was seen among treatments or weeks and all values remained within the reference range (Animal Health Laboratory, Guelph, Canada).…”