2000
DOI: 10.1046/j.1523-5378.2000.00001.x
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Quantitation of Helicobacter pylori in the Stomach Using Quantitative Polymerase Chain Reaction Assays

Abstract: The ability to rapidly quantitate H. pylori from gastric mucosa should be useful to investigate the role of H. pylori density and infection outcome, as well as to monitor the effectiveness of antibiotic treatment or vaccines against H. pylori.

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Cited by 7 publications
(9 citation statements)
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“…To isolate H. pylori DNA from fresh or frozen biopsy samples of the stomach, three methods have been used: kits from commercial companies, the proteinase K-phenol method, and a boiling-water method. The best of the three methods for obtaining a high yield or purity of H. pylori DNA has been shown to be the classical proteinase K-phenol method (10,18,19,25,44,51,56), which is supported by our results. Although we showed little difference in DNA degradation between DNA isolated with the commercial kit and that isolated with the revised proteinase K-phenol method, the revised proteinase K-phenol method produced a much higher yield.…”
Section: Discussionsupporting
confidence: 85%
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“…To isolate H. pylori DNA from fresh or frozen biopsy samples of the stomach, three methods have been used: kits from commercial companies, the proteinase K-phenol method, and a boiling-water method. The best of the three methods for obtaining a high yield or purity of H. pylori DNA has been shown to be the classical proteinase K-phenol method (10,18,19,25,44,51,56), which is supported by our results. Although we showed little difference in DNA degradation between DNA isolated with the commercial kit and that isolated with the revised proteinase K-phenol method, the revised proteinase K-phenol method produced a much higher yield.…”
Section: Discussionsupporting
confidence: 85%
“…Although the kinetic methods have several advantages over the coamplification methods (13,43), the few published studies of quantitation of H. pylori in biopsy samples (18,39,44,45) have used the coamplification method. This is the first report of real-time Q-PCR to detect H. pylori.…”
Section: Discussionmentioning
confidence: 99%
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