Real-Time Quantitative PCR for Detection of<i>Helicobacter pylori</i>

He, Qing; Wang, Jianping; Osato, Michael S.; Lachman, Lawrence B.

doi:10.1128/jcm.40.10.3720-3728.2002

Cited by 106 publications

(83 citation statements)

References 53 publications

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“…pylori density has been found to correlate with the degree of gastric-mucosal inflammation in children (Elitsur et al, 2002). Different authors have used different methods for scoring H. pylori density, including histology, culture, real-time quantitative PCR-based techniques or the urea breath test (Elitsur et al, 2002;Lai et al, 2003;Atherton et al, 1996;Abbas et al, 2001;He et al, 2002). One disadvantage of the invasive tests can be the patchy distribution of H. pylori in gastric mucosa (Vinette et al, 2004); however, H. pylori density has often been evaluated by invasive tests.…”

Section: Resultsmentioning

confidence: 99%

Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

Boyanova

Lazarova²,

Jelev³

et al. 2007

Journal of Medical Microbiology

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The aims of the study were to evaluate the incidence of Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children from 1996 to 2006, to analyse the performance of diagnostic tests, and to look at H. pylori density in specimens by culture. Antral specimens from children with chronic gastritis (n5513), peptic ulcers (n554) and other diseases (n591) were evaluated by direct Gram staining (DGS), in-house rapid urease test (RUT) and culture. The living environment and semi-quantitative H. pylori density were assessed in 188 and 328 children, respectively. H. pylori infection was found in children with ulcers (77.8 %), chronic gastritis (64.5 %) and other diseases (36.3 %). Half (51.4 %) of patients aged 1-5 years and 77.4 % of those aged 16-17 years were H. pylori-positive. Of all children, 328 (49.8 %) showed positive DGS, 184 (28 %) had a positive RUT, and 386 (58.7 %) were culture-positive. Unlike gastric mucus specimens, frozen biopsy specimens provided reliable diagnosis. H. heilmannii was observed in two (0.3 %) children. High H. pylori density (growth into all quadrants of plates) was found in 18 % of 328 children evaluated, involving 31 % of ulcer and 16.7 % of non-ulcer patients. H. pylori infection was more common in rural children with chronic gastritis (91.3 %) than in the remainder (66.7 %). In conclusion, H. pylori infection was common in symptomatic Bulgarian children. The infection prevalence was .77 % in patients aged 16-17 years, in children with a duodenal ulcer, and in rural patients. H. heilmannii infection was uncommon. The performance of the bacterial culture was good. The impact of H. pylori density on the clinical expression and eradication of the infection requires further evaluation. The results highlight the need for routine H. pylori diagnosis in rural children with chronic gastritis. INTRODUCTIONHelicobacter pylori infection is associated with gastroduodenal diseases in humans (Matysiak-Budnik et al., 2006). The incidence of the infection in children ranges from 10 to 80 %, and is high in developing and some other countries (Torres, 2000; Janulaityte-Gunther et al., 2005;Kato et al., 2004). H. pylori infection is most often intrafamilial and spreads by oral-oral or faecal-oral transmission (Megraud, 2003;Mladenova et al., 2006). Helicobacter heilmannii can also infect the human stomach. The incidence (usually ,1 %) of this zoonotic infection in dyspeptic patients is much lower than that caused by H. pylori (Ierardi et al., 2001;Okiyama et al., 2005). There are, however, only a few reports concerning H. heilmannii incidence in children (Coman et al., 1996;Mention et al., 1999; Sykora et al., 2004).The aims of the present study were to assess the incidence and characteristics of H. pylori and H. heilmannii infections in untreated Bulgarian children over a period of 10 years, according to age, sex, disease and living environment; to analyse the performance of diagnostic tests according to the type of antral specimen; and to evaluate H. pylori density in the biopsy spec...

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Section: Resultsmentioning

confidence: 99%

Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

Boyanova

Lazarova²,

Jelev³

et al. 2007

Journal of Medical Microbiology

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“…1,8,[19][20][21][22] Although culture from biopsy specimens has the potential of leading to a high sensitivity (>90%) under optimal conditions, 1,5,12 the literature reports sensitivity values of culture varying from 50% to 70% in experienced laboratories. 1,22 Our sensitivity (87.4%) seems to be comparable or better than the average of published values, 1 but still renders 12.6% falsely negative results, only reflecting the fastidious character of the bacterium and the inevitable suboptimal accuracy of the method in detecting active H. pylori infection in this type of unselected patient material.…”

Section: Discussionmentioning

confidence: 99%

“…1 Genetic methods can also be used to detect H. pylori in gastric biopsies and are recently shown to increase sensitivity of H. pylori detection compared to culture. 7,8 Besides accurately detecting H. pylori encompassing also non-culturable coccoid forms, 9 polymerase chain reaction (PCR) can be employed for strain characterization by detecting genes encoding pathogenic factors and identifying certain resistance traits by detecting microbial gene mutations. 10 PCR entails no special requirements in processing and transport, enabling a rapid and probably a cost-effective diagnosing of H. pylori, overcoming the delay associated with conventional culture methods.…”

Section: Introductionmentioning

confidence: 99%

Polymerase chain reaction versus culture in the diagnosis of Helicobacter pylori infection

Johannessen

Bergh

Jianu

et al. 2013

Gastroenterol Insights

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In the management of Helicobacter pyloriinduced gastroduodenal disease, a pilot study at our hospital (St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway) revealed that culture often seemed to fail compared to the polymerase chain reaction (PCR). A more thorough evaluation was therefore undertaken. We included 201 patients referred to upper gastrointestinal endoscopy in the period [2002][2003][2004]. Serology, biopsy rapid urease test, culture and PCR were performed. Conventional PCR was performed using the ureC, vacA and cagA genes, and real-time PCR for ureC. A diagnostic standard was defined on the basis of all four tests, and all four tests were then compared to this standard. One hundred eleven patients were deemed H. pylori-positive by the defined diagnostic standard, and 90 were labeled negative. Compared to this standard, culture showed a sensitivity of 87.4%, which was significantly lower than PCR at 99.1% (P<0.001). Culture showed a perfect specificity of 100%, which was significantly better than PCR at 97.8%. ureC was the gene with the best sensitivity (94.6% in conventional PCR, 97.3% in real-time PCR). vacA sensitivity was 87.4%, which is significantly lower than ureC (P<0.001). cagA was present in 37.8% of our H. pylori-positive patients. By real-time PCR a significantly lower cycle threshold was observed in antral biopsies than in corpal biopsies, indicating a higher H. pylori DNA template concentration in antral biopsies. PCR-testing for H. pylori is faster and significantly more sensitive than culture. Culture on the other hand was significantly more specific than PCR in our hand.

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“…These methods allow rapid and accurate quantification of H. pylori and have increased in importance because they can facilitate the monitoring of Genetics and Molecular Biology, 30, 2, 431-434 (2007) Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br therapy and even measure the effectiveness of vaccines against H. pylori (He et al, 2002).…”

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confidence: 99%

“…Recently, real-time PCR (RT-PCR) quantitative assays have been developed to quantify H. pylori in gastric and stool samples (He et al, 2002;Kobayashi et al, 2002;Lascols et al, 2003;Mikula et al, 2003;SchabereiterGurtner et al, 2004). These methods allow rapid and accurate quantification of H. pylori and have increased in importance because they can facilitate the monitoring of Genetics and Molecular Biology, 30, 2, 431-434 (2007) Copyright by the Brazilian Society of Genetics.…”

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confidence: 99%

Quantitative real-time PCR for the clinical detection of Helicobacter pylori

et al. 2007

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Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according to the references methods, 16 were H. pylori-positive by PCR, 10 of which were patients who had received H. pylori eradication therapy and 6 were untreated patients. Based on these findings we recommend that RT-PCR should be use in addition to standard methods in clinical studies to monitor the results of H. pylori eradication therapy.

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Real-Time Quantitative PCR for Detection ofHelicobacter pylori

Abstract: Helicobacter pylori is one of the most common chronic infections in humans, in whom it is a key etiological factor in peptic ulcer disease, gastric mucosa-associated lymphoid tissue lymphoma, and gastric adenocarcinoma.

Cited by 106 publications

References 53 publications

Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

Polymerase chain reaction versus culture in the diagnosis of Helicobacter pylori infection

Quantitative real-time PCR for the clinical detection of Helicobacter pylori

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