2002
DOI: 10.1128/jcm.40.10.3720-3728.2002
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Real-Time Quantitative PCR for Detection ofHelicobacter pylori

Abstract: Helicobacter pylori is one of the most common chronic infections in humans, in whom it is a key etiological factor in peptic ulcer disease, gastric mucosa-associated lymphoid tissue lymphoma, and gastric adenocarcinoma.

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Cited by 106 publications
(83 citation statements)
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“…pylori density has been found to correlate with the degree of gastric-mucosal inflammation in children (Elitsur et al, 2002). Different authors have used different methods for scoring H. pylori density, including histology, culture, real-time quantitative PCR-based techniques or the urea breath test (Elitsur et al, 2002;Lai et al, 2003;Atherton et al, 1996;Abbas et al, 2001;He et al, 2002). One disadvantage of the invasive tests can be the patchy distribution of H. pylori in gastric mucosa (Vinette et al, 2004); however, H. pylori density has often been evaluated by invasive tests.…”
Section: Resultsmentioning
confidence: 99%
“…pylori density has been found to correlate with the degree of gastric-mucosal inflammation in children (Elitsur et al, 2002). Different authors have used different methods for scoring H. pylori density, including histology, culture, real-time quantitative PCR-based techniques or the urea breath test (Elitsur et al, 2002;Lai et al, 2003;Atherton et al, 1996;Abbas et al, 2001;He et al, 2002). One disadvantage of the invasive tests can be the patchy distribution of H. pylori in gastric mucosa (Vinette et al, 2004); however, H. pylori density has often been evaluated by invasive tests.…”
Section: Resultsmentioning
confidence: 99%
“…1,8,[19][20][21][22] Although culture from biopsy specimens has the potential of leading to a high sensitivity (>90%) under optimal conditions, 1,5,12 the literature reports sensitivity values of culture varying from 50% to 70% in experienced laboratories. 1,22 Our sensitivity (87.4%) seems to be comparable or better than the average of published values, 1 but still renders 12.6% falsely negative results, only reflecting the fastidious character of the bacterium and the inevitable suboptimal accuracy of the method in detecting active H. pylori infection in this type of unselected patient material.…”
Section: Discussionmentioning
confidence: 99%
“…1 Genetic methods can also be used to detect H. pylori in gastric biopsies and are recently shown to increase sensitivity of H. pylori detection compared to culture. 7,8 Besides accurately detecting H. pylori encompassing also non-culturable coccoid forms, 9 polymerase chain reaction (PCR) can be employed for strain characterization by detecting genes encoding pathogenic factors and identifying certain resistance traits by detecting microbial gene mutations. 10 PCR entails no special requirements in processing and transport, enabling a rapid and probably a cost-effective diagnosing of H. pylori, overcoming the delay associated with conventional culture methods.…”
Section: Introductionmentioning
confidence: 99%
“…These methods allow rapid and accurate quantification of H. pylori and have increased in importance because they can facilitate the monitoring of Genetics and Molecular Biology, 30, 2, 431-434 (2007) Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br therapy and even measure the effectiveness of vaccines against H. pylori (He et al, 2002).…”
mentioning
confidence: 99%
“…Recently, real-time PCR (RT-PCR) quantitative assays have been developed to quantify H. pylori in gastric and stool samples (He et al, 2002;Kobayashi et al, 2002;Lascols et al, 2003;Mikula et al, 2003;SchabereiterGurtner et al, 2004). These methods allow rapid and accurate quantification of H. pylori and have increased in importance because they can facilitate the monitoring of Genetics and Molecular Biology, 30, 2, 431-434 (2007) Copyright by the Brazilian Society of Genetics.…”
mentioning
confidence: 99%