Quantitation of genetically modified organisms in food

Detection and quantification of Roundup Ready® soybean residues in sausage samples by conventional and real-time PCR

“…The DNA-based methods make use of the polymerase chain reaction (PCR) technique and can be applied both to products in natura and to highly processed foods. However, some products cause many inconveniences for extracting DNA with satisfactory amplificability (VAITILINGOM et al, 1999;LIPP et al, 2001;HÜBNER;STUDER;LÜTHY, 1999;VOLLENHOFER et al, 1999;CARDARELLI et al, 2005).…”

Detection of Genetically Modified Organisms in Foods by DNA Amplification Techniques

“…65 It has been suggested that the use of quantitative methods would help to improve the reproducibility between laboratories. 65,99 Several validation studies of quantitative PCR methods have been reported during the last few years, either by QC-PCR 99 …”

“…The remaining variability in the results could be attributed to deficient homogenisation of the samples or to inaccurate quantification of the amplification products. 70 However, most of these problems could probably be overcome by the use of appropriate internal controls. …”

Control of GMO Content in Seed and Feed

“…The sample sizes mentioned in Hübner et al (2001) when testing for compliance with a 1 % labelling threshold give rise to the laboratory sample weights shown in However, for the specific detection of GMOs in feed the expected ISO/DIS standard on sampling methods for the detection of GMOs in food would also seem to be applicable.…”