Quantitation of fumonisin B 1 and B 2 in feed using FMOC pre-column derivatization with HPLC and fluorescence detection

Smith, Lori; Francis, Kyle A.; Johnson, Joseph Taber; Gaskill, Cynthia L.

doi:10.1016/j.foodchem.2017.04.142

Cited by 22 publications

(16 citation statements)

References 14 publications

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“…When this detector is employed, some mycotoxins need derivatization in order to improve the response [31]. This is the case of AFB1 and AFG1 [5] and of fumonisin B1 (FB1) and fumonisin B2 (FB2) [32][33][34]. The FLD has been used in the determination of mycotoxins in several raw materials, such as cereals [35][36][37], even in detection of multiple mycotoxins [38].…”

Section: Discussionmentioning

confidence: 99%

“…A search of the literature in PubMed, including the search terms "Mycotoxin AND feed AND fluorescence," limited to the last 10 years, revealed a total of 20 studies on the analysis of mycotoxins in feed (no raw materials) using LC-FLD [4,5,9,16,17,30,[32][33][34][39][40][41][42][43][44][45][46][47][48][49]. AFs are the most widely studied mycotoxins [4,5,9,16,17,30,[40][41][42][43], particularly AFB1, as a single mycotoxin [44,[47][48][49].…”

Section: Discussionmentioning

confidence: 99%

“…AFs are the most widely studied mycotoxins [4,5,9,16,17,30,[40][41][42][43], particularly AFB1, as a single mycotoxin [44,[47][48][49]. Other mycotoxins studied are OTA [4,9,17,[44][45][46], ZEA [17,33,42,44,47], FB1 and FB2 [32][33][34], and T-2 toxin [33]. Only one study achieves multi-mycotoxin quantification (including mycotoxins from different chemical groups) of AFB1, AFB2, AFG1, AFG2, OTA, and ZEA using immunoaffinity columns (IAC) [39].…”

Section: Discussionmentioning

confidence: 99%

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Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix

Muñoz-Solano

González-Peñas

2020

Toxins

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Mycotoxins are toxic compounds for humans and animals that are produced by fungi. Mycotoxin contamination in feed is a global safety concern and effective control of these compounds in this matrix is needed. This study proposes a simple, cost-effective analytical method based on liquid chromatography coupled with a fluorescence detector, which is suitable for the routine monitoring of some of the most important mycotoxins in feed: aflatoxins (G2, G1, B2, and B1), zearalenone, and ochratoxins A and B. Mycotoxin extraction, chromatographic separation and quantification are carried out simultaneously for all mycotoxins. The extraction procedure is performed using acetonitrile, water and orthophosphoric acid (80:19:1). Purification of the extract is carried out using an OASIS PRIME HLB solid-phase extraction cartridge followed by a dispersive liquid–liquid microextraction procedure. Aflatoxins G1 and B1 are derivatized post-column (photochemical reactor at 254 nm) to increase their signal. The method has been validated in feed for pigs, cows, sheep, and poultry with very satisfactory results. The detection limits are 2 μg/kg for aflatoxins B1 and G1, 0.64 μg/kg for aflatoxins B2 and G2, 42 μg/kg for zearalenone, and 5 μg/kg for ochratoxins A and B. These values are low enough to allow for monitoring of these mycotoxins in feed. Global recovery values were between 73.6% and 88.0% for all toxins with a relative standard deviation (RSD) % < 7%. This methodology will facilitate laboratory control and analysis of mycotoxins in feed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix

Muñoz-Solano

González-Peñas

2020

Toxins

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“…For many years, liquid chromatography with fluorescence or UV detection were the “gold standard” in mycotoxin control. Most of the published and normalized methods were based on immunoaffinity clean‐up and allowed specific, single‐analyte analysis with sufficient performance.…”

Section: Introductionmentioning

confidence: 99%

“…Article Related Abbreviations: AFB 1 , aflatoxin B 1 ; AFLB1-IS, aflatoxin B1 13 C 17 ; DON, deoxynivalenol; FB 1 , fumonisin B 1 ; FB1-IS, fumonisin B1 13 C 34 ; FB 2 , fumonisin B 2 ; HT-2, HT-2 toxin; HT-2-IS, toxin HT-2 13 C 22 ; IAC, immunoaffinity chromatography; MIX8 VL, working standard solution for analytes; MIX8 VL IS, working standard solution for internal standards; OTA, ochratoxin A; PBS, phosphate buffered saline; T-2, T-2 toxins; T-2-IS, toxin T-2 13 For many years, liquid chromatography with fluorescence or UV detection were the "gold standard" in mycotoxin control. Most of the published and normalized methods were based on immunoaffinity clean-up [6,7] and allowed specific, singleanalyte analysis with sufficient performance.…”

Section: Introductionmentioning

confidence: 99%

Multiple mycotoxins analysis in animal feed with LC‐MS/MS: Comparison of extract dilution and immunoaffinity clean‐up

Jedziniak

Panasiuk

Pietruszka

et al. 2019

J of Separation Science

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The aim of this study was a performance comparison of two clean‐up procedures (dilutions versus immunoaffinity columns) in the simultaneous determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1 & B2, ochratoxin A, toxin T‐2 & HT‐2 and zearalenone) in the animal feed. After extraction the analytes were separated on a Kinetex Biphenyl column with a gradient elution using methanol/0.01 M ammonium acetate as a mobile phase and analyzed with the LC‐MS/MS technique. Both of the procedures were validated by analysis of a series of spiked feed samples (n = 6) at three different concentration levels. Better signal to noise ratios were observed for immunoaffinity clean‐up. The recoveries of analyses were in the range 88–110% for the dilution procedure and 78–120% for the immunoaffinity clean‐up. The dilution procedure was more precise (coefficient of variation of the within‐laboratory reproducibility for it was 7.8–22.4% in comparison to 12–35.5% for the immunoaffinity clean‐up. The results show that both procedures fulfilled the requirements for mycotoxin analysis and can be used successfully in multi‐analyte determination. Although the dilution procedure shows better precision and trueness, the immunoaffinity clean‐up procedure can have advantages in more complex feed samples thanks to lower matrix effect and limits of detections.

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Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B₁ in Wheat Samples

et al. 2022

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The development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2‐GLuc and A2‐NLuc) is detailed. The assay showing the best performance, A2‐NLuc, with a limit of detection of 0.61 ng mL−1 and a dynamic range from 1.9 to 95 ng mL−1, was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109 %, with relative standard deviations lower than 14 %. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.

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Quantitation of fumonisin B 1 and B 2 in feed using FMOC pre-column derivatization with HPLC and fluorescence detection

Cited by 22 publications

References 14 publications

Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix

Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix

Multiple mycotoxins analysis in animal feed with LC‐MS/MS: Comparison of extract dilution and immunoaffinity clean‐up

Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B₁ in Wheat Samples

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Quantitation of fumonisin B 1 and B 2 in feed using FMOC pre-column derivatization with HPLC and fluorescence detection

Cited by 22 publications

References 14 publications

Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix

Mycotoxin Determination in Animal Feed: An LC-FLD Method for Simultaneous Quantification of Aflatoxins, Ochratoxins and Zearelanone in This Matrix

Multiple mycotoxins analysis in animal feed with LC‐MS/MS: Comparison of extract dilution and immunoaffinity clean‐up

Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples

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Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B₁ in Wheat Samples